Search
Fanglian He Bio-protocol
205 protocols

Reviewer
Cheng Zhang
  • University of Pittsburgh
Research focus
  • Biophysics
2 Protocols reviewed
[3H]-Spiperone Saturation Binding to Dopamine D2, D3 and D4 Receptors
Authors:  Jan-Peter van Wieringen and Martin C. Michel, date: 10/20/2013, view: 5763, Q&A: 0
This protocol is intended for use in 96 well plates (1,200 μl wells) but it can similarly be applied to standard test tubes (Levant, 2007). D2, D3, and D4 dopamine receptors are members of the D2-like ...
More >
[3H]-Spiperone Competition Binding to Dopamine D2, D3 and D4 Receptors
Authors:  Jan-Peter van Wieringen and Martin C. Michel, date: 10/20/2013, view: 4767, Q&A: 0
This protocol is intended for use in 96 well plates (1,200 μl wells) but it can similarly be applied to standard test tubes (Levant, 2007). D2, D3, and D4 dopamine receptors are members of the D2-like ...
More >
5 Protocols edited
Adenosine A2A Receptor Ligand Binding Experiments by Using Real-time Single-cell FRET
Authors:  Víctor Fernández-Dueñas, Kenneth A. Jacobson and Francisco Ciruela, date: 03/20/2014, view: 5892, Q&A: 0
We designed a fluorescence resonance energy transfer (FRET)-based approach to study the ligand binding constants of the adenosine A2A receptor (A2AR). Our assay is based in the interaction of a fluorescent A2AR ...
More >
Synthesis of the Adenosine A2A Receptor Fluorescent Agonist MRS5424
Authors:  Kenneth A. Jacobson and Francisco Ciruela, date: 03/20/2014, view: 4658, Q&A: 0
MRS5424 is a functional fluorescent agonist for the adenosine A2A receptor (A2AR) in which the fluorescent dye Alexa Fluor 532 is covalently attached to the A2AR agonist ...
More >