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David Camerini
1 protocol
Hugh Fisher
1 protocol
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Alvaro E. Galvis
Department of Molecular Biology & Biochemistry, University of California, USA, USA,
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1 Protocol published
NP-40 Fractionation and Nucleic Acid Extraction in Mammalian Cells
Authors:
Alvaro E. Galvis
,
Hugh E. Fisher
and
David Camerini
,
date:
10/20/2017,
view:
1995,
Q&A:
1
This technique allows for efficient, highly purified cytoplasmic and nuclear-associated compartment fractionation utilizing NP-40 detergent in mammalian cells. The nuclear membrane is not disturbed during the fractionation thus leaving all nuclear ...
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Julia Guenther
Medical University of Innsbruck
Nice protocol! I will try it out in the coming weeks. I noticed, however, that in Procedure A - Steps 11 & 12 suggest the use of 500 ml. I am assuming you mean 500 µl. It that correct?
11/3/2017 5:51:56 AM
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Alvaro E. Galvis
Department of Molecular Biology & Biochemistry, University of California, USA, USA,
Yes, that's correct
2018-03-04 21:19:50
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