Hi, I tried this protocol hoping to extract nuclei, thus I only completed the NP-40 fractionation part using 293T cells, after which I did western blot with Anti-Actin antibody produced in mouse and Anti-Lamin B antibody produced in rabbit. But the reseult shows that the nuclei part still had cytoplasm component. I was confused and would you like to help me figure out why?
11/4/2018 5:17:30 PM Reply