Steven Boeynaems —BIO-PROTOCOL

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Ludo Van Den Bosch Department of Neuroscience, Department of Neuroscience, Belgium
2 protocols

Peter Tompa VIB, Center for Structural Biology (CSB), Vrije Universiteit Brussel (VUB), Belgium
1 protocol

Mathias De Decker KU Leuven–University of Leuven, Department of Neurosciences, Experimental Neurology and Leuven Research Institute for Neuroscience and Disease (LIND), Belgium
1 protocol

Alessandro Didonna University of California, San Francisco
48 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
48 protocols

Amberley Stephens Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge
3 protocols

Brahma Mulugu Brahma Mulugu
4 protocols

Editor

Steven Boeynaems

Post-Doc, Stanford University

Research focus

Molecular biology
neurodegeneration - protein phase transition - tandem repeats

1 Author merit
Reviewer merit
Editor merit

Lab Protocol Hub

Gitler Lab

Education

PhD, University of Leuven, 2017

Lab information

http://www.stevenboeynaems.com

Publications

https://scholar.google.com/citations?user=ZIrl7WgAAAAJ&hl=en

1 Protocol published

Arginine-rich Peptides Can Actively Mediate Liquid-liquid Phase Separation

Authors: Steven Boeynaems, Mathias De Decker, Peter Tompa and Ludo Van Den Bosch, date: 09/05/2017, view: 10291, Q&A: 0

Studying liquid-liquid phase separation (LLPS) of proteins provides key insights into the biogenesis of membraneless organelles and pathological protein aggregation in disease. We have established a protocol for inducing the phase separation of ...

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4 Protocols reviewed

Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus

Authors: Gabriele Stankeviciute and Eric A. Klein, date: 11/05/2019, view: 2703, Q&A: 0

The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape. Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the ...

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Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices

Authors: Panayiotis Tsokas, Bruno Rivard, Changchi Hsieh, James E. Cottrell, André Antonio Fenton and Todd Charlton Sacktor, date: 10/05/2019, view: 2301, Q&A: 0

The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in ...

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5 Protocols edited

A Quantitative Assay to Measure Stress Granule Association of Proteins and Peptides in Semi-permeabilized Human Cells

Authors: Saskia Hutten and Dorothee Dormann, date: 12/20/2020, view: 1723, Q&A: 0

Stress granules (SGs) are membrane-less organelles that form in the cytoplasm through phase separation, in response to diverse stressors. SGs contain translationally stalled mRNAs, proteins involved in translation, and various RNA-binding proteins

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Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS

Authors: Emma Robinson, James Shorter and Lin Guo, date: 08/20/2020, view: 2005, Q&A: 0

The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ...

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