Yangbin Gao —BIO-PROTOCOL

Home About Us For Authors Submit Protocol Archive

Yunde Zhao National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol

Rongchen Wang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol

Tao Zhang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol

Yangbin Gao

Section of Cell and Developmental Biology, University of California, USA

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Production of Guide RNAs in vitro and in vivo for CRISPR Using Ribozymes and RNA Polymerase II Promoters

Authors: Tao Zhang, Yangbin Gao, Rongchen Wang and Yunde Zhao, date: 02/20/2017, view: 8663, Q&A: 0

CRISPR/Cas9-mediated genome editing relies on a guide RNA (gRNA) molecule to generate sequence-specific DNA cleavage, which is a prerequisite for gene editing. Here we establish a method that enables production of gRNAs from any promoters, in any ...

More >