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A peer-reviewed protocol journal. No publication fee; no access fee.
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Yunde Zhao
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol
Rongchen Wang
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol
Tao Zhang
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, China
1 protocol
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Yangbin Gao
Section of Cell and Developmental Biology, University of California, USA
Research focus
Molecular biology
Contributions
1
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1 Protocol published
Production of Guide RNAs
in vitro
and
in vivo
for CRISPR Using Ribozymes and RNA Polymerase II Promoters
Authors:
Tao Zhang
,
Yangbin Gao
,
Rongchen Wang
and
Yunde Zhao
,
date:
02/20/2017,
view:
13297,
Q&A:
0
CRISPR/Cas9-mediated genome editing relies on a guide RNA (gRNA) molecule to generate sequence-specific DNA cleavage, which is a prerequisite for gene editing. Here we establish a method that enables production of gRNAs from any promoters, in any ...
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