Noriyoshi Sakai Genetic Strains Research Center, National Institute of Genetics, and Department of Genetics, School of Life Science, the Graduate University for Advanced Studies (SOKENDAI), Japan
1 protocol

Toshihiro Kawasaki
  • Genetic Strains Research Center, National Institute of Genetics, and Department of Genetics, School of Life Science, the Graduate University for Advanced Studies (SOKENDAI), Japan
Research focus
  • Stem cell
  • 1 Author merit


Ph.D., Kumamoto University, 2006

Current position

Assistant professor in Genetic Strains Research Center, National Institute of Genetics, Mishima, Japan


  1. Kawasaki, T., Siegfried, K. R. and Sakai, N. (2016). Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture. Development 143(4): 566-574.

  2. Hyakutake, K., Kawasaki, T., Zhang, J., Kubota, H., Abe, S. I. and Takamune, K. (2015). Asymmetrical allocation of JAK1 mRNA during spermatogonial stem cell division in Xenopus laevis. Dev Growth Differ.

  3. Nishimura, T., Herpin, A., Kimura, T., Hara, I., Kawasaki, T., Nakamura, S., Yamamoto, Y., Saito, T. L., Yoshimura, J., Morishita, S., Tsukahara, T., Kobayashi, S., Naruse, K., Shigenobu, S., Sakai, N., Schartl, M. and Tanaka, M. (2014). Analysis of a novel gene, Sdgc, reveals sex chromosome-dependent differences of medaka germ cells prior to gonad formation. Development 141(17): 3363-3369.

  4. Shinya, M., Kobayashi, K., Masuda, A., Tokumoto, M., Ozaki, Y., Saito, K., Kawasaki, T., Sado, Y. and Sakai, N. (2013). Properties of gene knockdown system by vector-based siRNA in zebrafish. Dev Growth Differ 55(9): 755-765.

  5. Saito, K., Sakai, C., Kawasaki, T. and Sakai, N. (2014). Telomere distribution pattern and synapsis initiation during spermatogenesis in zebrafish. Dev Dyn 243(11): 1448-1456.

  6. Kawasaki, T., Saito, K., Sakai, C., Shinya, M. and Sakai, N. (2012). Production of zebrafish offspring from cultured spermatogonial stem cells. Genes Cells 17(4): 316-325.

  7. Ozaki, Y., Saito, K., Shinya, M., Kawasaki, T. and Sakai, N. (2011). Evaluation of Sycp3, Plzf and Cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish. Gene Expr Patterns 11(5-6): 309-315.

  8. Kawasaki, T., Saito, K., Shinya, M., Olsen, L. C. and Sakai, N. (2010). Regeneration of spermatogenesis and production of functional sperm by grafting of testicular cell aggregates in Zebrafish (Danio rerio). Biol Reprod 83(4): 533-539.

  9. Kawasaki, T., Saito, K., Mitsui, K., Ikawa, M., Yamashita, M., Taniguchi, Y., Takeda, S., Mitani, K. and Sakai, N. (2009). Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors. Zebrafish 6(3): 253-258.

  10. Eto, K., Eda, K., Hayano, M., Goto, S., Nagao, K., Kawasaki, T., Kashimura, H., Tarui, H., Nishimura, O., Agata, K. and Abe, S. (2009). Reduced expression of an RNA-binding protein by prolactin leads to translational silencing of programmed cell death protein 4 and apoptosis in newt spermatogonia. J Biol Chem 284(35): 23260-23271.

  11. Kawasaki, T., Imura, F., Nakada, A., Kubota, H., Sakamaki, K., Abe, S. and Takamune, K. (2006). Functional demonstration of the ability of a primary spermatogonium as a stem cell by tracing a single cell destiny in Xenopus laevis. Dev Growth Differ 48(8): 525-535.

  12. Takamune, K., Kawasaki, T. and Ukon, S. (2001). The first and the second mitotic phases of spermatogonial stage in Xenopus laevis: Secondary spermatogonia which have differentiated after completion of the first mitotic phase acquire an ability of mitosis to meiosis conversion. Zoological Science. 18. 577-583.

1 Protocol published
Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish
Authors:  Toshihiro Kawasaki and Noriyoshi Sakai, date: 11/05/2016, view: 5672, Q&A: 0
Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These ...
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