Ikuko Hara-Nishimura Department of Botany, Graduate School of Science, Kyoto University, Japan
1 protocol

Tomoo Shimada Department of Botany, Graduate School of Science, Kyoto University, Japan
1 protocol

Takashi Kawase Department of System Science, Graduate School of Informatics, Kyoto University, Japan
1 protocol

Shigeo S. Sugano
  • Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, Japan
Research focus
  • Plant science
  • 1 Author merit


Ph.D., Graduate school of Science, Kyoto University, Japan, 2013

Current position

Project Assistant Professor, Tokushima University, Japan, 2014


  1. Kawase, T., Sugano, S. S., Shimada, T. and Hara-Nishimura, I. (2015). A direction-selective local-thresholding method, DSLT, in combination with a dye-based method for automated three-dimensional segmentation of cells and airspaces in developing leaves. Plant J 81(2): 357-366.
  2. Sugano, S. S., Shirakawa, M., Takagi, J., Matsuda, Y., Shimada, T., Hara-Nishimura, I. and Kohchi, T. (2014). CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L. Plant Cell Physiol 55(3): 475-481.
  3. Tanaka, Y.*, Sugano, S. S.*, Shimada, T. and Hara-Nishimura, I. (2013). Enhancement of leaf photosynthetic capacity through increased stomatal density in Arabidopsis. New Phytol 198(3): 757-764. * equal contribution
  4. Shimada, T., Sugano, S. S. and Hara-Nishimura, I. (2011). Positive and negative peptide signals control stomatal density. Cell Mol Life Sci 68(12): 2081-2088.
  5. Sugano, S. S., Shimada, T., Imai, Y., Okawa, K., Tamai, A., Mori, M. and Hara-Nishimura, I. (2010). Stomagen positively regulates stomatal density in Arabidopsis. Nature 463(7278): 241-244.
1 Protocol published
Differential and Simultaneous Visualization of Cells and Airspaces in Plant Leaves
Authors:  Takashi Kawase, Shigeo S. Sugano, Tomoo Shimada and Ikuko Hara-Nishimura, date: 06/05/2016, view: 6411, Q&A: 0
This protocol is to differentially and simultaneously visualize both cells and airspaces in intact leaves and to create 3D structures of cells and airspaces from confocal images using the software DSLT_Demo (https://github.com/nslab2000/DSLT ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.