Peter E Burby —BIO-PROTOCOL

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Coauthors

Lyle Simmons Department of Molecular, Cellular, and Developmental Biology, Univerisity of Michigan, United States of America
1 protocol

Coreviewers

Rainer Melzer University College Dublin
9 protocols

Renate Weizbauer Carnegie Institution for Science
37 protocols

Reviewer

Peter E Burby

Graduate, Univerisity of Michigan

Research focus

Microbiology
DNA damage response, DNA repair, stress response

1 Author merit
Reviewer merit
Editor merit

Education

Bachelor's of Science, Univerisity of Michigan, 2012

Lab information

Lyle Simmons Lab
https://sites.lsa.umich.edu/simmons-lab/

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=Burby+PE

1 Protocol published

CRISPR/Cas9 Editing of the Bacillus subtilis Genome

Authors: Peter E. Burby and Lyle A. Simmons, date: 04/20/2017, view: 4471, Q&A: 0

A fundamental procedure for most modern biologists is the genetic manipulation of the organism under study. Although many different methods for editing bacterial genomes have been used in laboratories for decades, the adaptation of CRISPR/Cas9 ...

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1 Protocol reviewed

Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein

Authors: Prarthana Mohanraju, John van der Oost, Martin Jinek and Daan C. Swarts, date: 05/05/2018, view: 506, Q&A: 0

This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification ...

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