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Coauthors
Lyle Simmons Department of Molecular, Cellular, and Developmental Biology, Univerisity of Michigan, United States of America
1 protocol
Coreviewers
David Cisneros Umeå University
21 protocols

Juan Facundo Ayala National University of Rosario
6 protocols

Rainer Melzer University College Dublin
11 protocols

Renate Weizbauer Carnegie Institution for Science
40 protocols

Reviewer
Peter E Burby
  • Graduate, Univerisity of Michigan
Research focus
  • Microbiology
  • DNA damage response, DNA repair, stress response
  • 1 Author merit

Education

Bachelor's of Science, Univerisity of Michigan, 2012

Lab information

Lyle Simmons Lab
https://sites.lsa.umich.edu/simmons-lab/

Publications

1 Protocol published
CRISPR/Cas9 Editing of the Bacillus subtilis Genome
Authors:  Peter E. Burby and Lyle A. Simmons, date: 04/20/2017, view: 6139, Q&A: 3
A fundamental procedure for most modern biologists is the genetic manipulation of the organism under study. Although many different methods for editing bacterial genomes have been used in laboratories for decades, the adaptation of CRISPR/Cas9 ...
2 Protocols reviewed
CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum
Authors:  Jiao Liu, Yu Wang, Ping Zheng and Jibin Sun, date: 10/05/2018, view: 105, Q&A: 0
Corynebacterium glutamicum is a versatile workhorse for industrial bioproduction of many kinds of chemicals and fuels, notably amino acids. Development of advanced genetic engineering tools is urgently demanded for systems metabolic ...
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Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
Authors:  Prarthana Mohanraju, John van der Oost, Martin Jinek and Daan C. Swarts, date: 05/05/2018, view: 1670, Q&A: 3
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification ...
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