Lyle Simmons Department of Molecular, Cellular, and Developmental Biology, Univerisity of Michigan, United States of America
1 protocol
David Cisneros Umeå University
23 protocols

Juan Facundo Ayala National University of Rosario
9 protocols

Marisa Rosa Bio-protocol.org
52 protocols

Rainer Melzer University College Dublin
12 protocols

Reviewer
Peter E Burby
  • Graduate, Univerisity of Michigan
Research focus
  • Microbiology
  • DNA damage response, DNA repair, stress response
  • 1 Author merit

Education

Bachelor's of Science, Univerisity of Michigan, 2012

Lab information

Lyle Simmons Lab
https://sites.lsa.umich.edu/simmons-lab/

Publications

1 Protocol published
CRISPR/Cas9 Editing of the Bacillus subtilis Genome
Authors:  Peter E. Burby and Lyle A. Simmons, date: 04/20/2017, view: 9156, Q&A: 4
A fundamental procedure for most modern biologists is the genetic manipulation of the organism under study. Although many different methods for editing bacterial genomes have been used in laboratories for decades, the adaptation of CRISPR/Cas9 ...
3 Protocols reviewed
Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
Authors:  Jarrett Man and Madelaine E. Bartlett, date: 05/05/2019, view: 94, Q&A: 0
CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
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CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum
Authors:  Jiao Liu, Yu Wang, Ping Zheng and Jibin Sun, date: 10/05/2018, view: 1169, Q&A: 0
Corynebacterium glutamicum is a versatile workhorse for industrial bioproduction of many kinds of chemicals and fuels, notably amino acids. Development of advanced genetic engineering tools is urgently demanded for systems metabolic ...
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