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Coauthors
Michela Perego The Wistar Institute
1 protocol

Chiara Castelli Department of Experimental Oncology and Molecular Medicine, Unit of Immunotherapy of Human Tumors, Fondazione IRCCS Istituto Nazionale dei Tumori, Italy
1 protocol

Francesca Rini Department of Experimental Oncology and Molecular Medicine, Unit of Immunotherapy of Human Tumors, Fondazione IRCCS Istituto Nazionale dei Tumori, Italy
1 protocol

Valeria Beretta Department of Experimental Oncology and Molecular Medicine, Unit of Immunotherapy of Human Tumors, Fondazione IRCCS Istituto Nazionale dei Tumori, Italy
1 protocol

Alessandra Tuccitto
  • Department of Experimental Oncology and Molecular Medicine, Unit of Immunotherapy of Human Tumors, Fondazione IRCCS Istituto Nazionale dei Tumori, Italy
  • 1 Author merit

Education

Master Degree in Biology Applied to Biomedical Research, State University of Milan, Italy, 2012

Current position

Fellow at Unit of Immunotherapy of Human Tumors, Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy

Publications

  1. Worsdorfer, P., Bosen, F., Gebhardt, M., Russ, N., Zimmermann, K., Komla Kessie, D., Sekaran, T., Egert, A., Ergun, S., Schorle, H., Pfeifer, A., Edenhofer, F. and Willecke, K. (2017). Abrogation of Gap Junctional Communication in ES Cells Results in a Disruption of Primitive Endoderm Formation in Embryoid Bodies. Stem Cells 35(4): 859-871.
  2. Worsdorfer, P., Mekala, S. R., Bauer, J., Edenhofer, F., Kuerten, S. and Ergun, S. (2017). The vascular adventitia: An endogenous, omnipresent source of stem cells in the body. Pharmacol Ther 171: 13-29.
  3. Scognamiglio, R., Cabezas-Wallscheid, N., Thier, M. C., Altamura, S., Reyes, A., Prendergast, A. M., Baumgartner, D., Carnevalli, L. S., Atzberger, A., Haas, S., von Paleske, L., Boroviak, T., Worsdorfer, P., Essers, M. A., Kloz, U., Eisenman, R. N., Edenhofer, F., Bertone, P., Huber, W., van der Hoeven, F., Smith, A. and Trumpp, A. (2016). Myc Depletion Induces a Pluripotent Dormant State Mimicking Diapause. Cell 164(4): 668-680.
  4. Bung, R., Worsdorfer, P., Thier, M. C., Lemke, K., Gebhardt, M. and Edenhofer, F. (2016). Partial Dedifferentiation of Murine Radial Glia-Type Neural Stem Cells by Brn2 and c-Myc Yields Early Neuroepithelial Progenitors. J Mol Biol 428(7): 1476-1483.
  5. Meyer, S., Worsdorfer, P., Gunther, K., Thier, M. and Edenhofer, F. (2015). Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells. J Vis Exp(101): e52831.
  6. Worsdorfer, P., Thier, M., Kadari, A. and Edenhofer, F. (2013). Roadmap to cellular reprogramming--manipulating transcriptional networks with DNA, RNA, proteins and small molecules. Curr Mol Med 13(5): 868-878.
  7. Hennen, E., Safina, D., Haussmann, U., Worsdorfer, P., Edenhofer, F., Poetsch, A. and Faissner, A. (2013). A LewisX glycoprotein screen identifies the low density lipoprotein receptor-related protein 1 (LRP1) as a modulator of oligodendrogenesis in mice. J Biol Chem 288(23): 16538-16545.
  8. Schmidt, V. J., Jobs, A., von Maltzahn, J., Worsdorfer, P., Willecke, K. and de Wit, C. (2012). Connexin45 is expressed in vascular smooth muscle but its function remains elusive. PLoS One 7(7): e42287.
  9. Thier, M., Worsdorfer, P., Lakes, Y. B., Gorris, R., Herms, S., Opitz, T., Seiferling, D., Quandel, T., Hoffmann, P., Nothen, M. M., Brustle, O. and Edenhofer, F. (2012). Direct conversion of fibroblasts into stably expandable neural stem cells. Cell Stem Cell 10(4): 473-479.
  10. Zlomuzica, A., Reichinnek, S., Maxeiner, S., Both, M., May, E., Worsdorfer, P., Draguhn, A., Willecke, K. and Dere, E. (2010). Deletion of connexin45 in mouse neurons disrupts one-trial object recognition and alters kainate-induced gamma-oscillations in the hippocampus. Physiol Behav 101(2): 245-253.
  11. Dobrowolski, R., Hertig, G., Lechner, H., Worsdorfer, P., Wulf, V., Dicke, N., Eckert, D., Bauer, R., Schorle, H. and Willecke, K. (2009). Loss of connexin43-mediated gap junctional coupling in the mesenchyme of limb buds leads to altered expression of morphogens in mice. Hum Mol Genet 18(15): 2899-2911.
  12. von Maltzahn, J., Kreuzberg, M. M., Matern, G., Euwens, C., Hoher, T., Worsdorfer, P. and Willecke, K. (2009). C-terminal tagging with eGFP yields new insights into expression of connexin45 but prevents rescue of embryonic lethal connexin45-deficient mice. Eur J Cell Biol 88(9): 481-494.
  13. Worsdorfer, P., Maxeiner, S., Markopoulos, C., Kirfel, G., Wulf, V., Auth, T., Urschel, S., von Maltzahn, J. and Willecke, K. (2008). Connexin expression and functional analysis of gap junctional communication in mouse embryonic stem cells. Stem Cells 26(2): 431-439.
  14. Schnichels, M., Worsdorfer, P., Dobrowolski, R., Markopoulos, C., Kretz, M., Schwarz, G., Winterhager, E. and Willecke, K. (2007). The connexin31 F137L mutant mouse as a model for the human skin disease erythrokeratodermia variabilis (EKV). Hum Mol Genet 16(10): 1216-1224.
  15. Urschel, S., Hoher, T., Schubert, T., Alev, C., Sohl, G., Worsdorfer, P., Asahara, T., Dermietzel, R., Weiler, R. and Willecke, K. (2006). Protein kinase A-mediated phosphorylation of connexin36 in mouse retina results in decreased gap junctional communication between AII amacrine cells. J Biol Chem 281(44): 33163-33171.
1 Protocol published
Melanoma Stem Cell Sphere Formation Assay
Authors:  Alessandra Tuccitto, Valeria Beretta, Francesca Rini, Chiara Castelli and Michela Perego, date: 04/20/2017, view: 3535, Q&A: 0
Self-renewal is the ability of cells to replicate themselves at every cell cycle. Throughout self-renewal in normal tissue homeostasis, stem cell number is maintained constant throughout life. Cancer stem cells (CSCs) share this ability with normal ...