Search
Coauthors
James Donahue Department of Surgery, University of Maryland School of Medicine, USA
1 protocol
Coreviewers
HongLok Lung University of Hong Kong
58 protocols

Tomas Aparicio Columbia University
5 protocols

Reviewer
Pornima Phatak
  • University of Maryland School of Medicine
Research focus
  • Cancer biology
  • 1 Author merit
1 Protocol published
Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets
Authors:  Pornima Phatak and James M Donahue, date: 05/05/2017, view: 3998, Q&A: 0
microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.
1 Protocol reviewed
An in vitro Co-culture System for the Activation of CD40 by Membrane-presented CD40 Ligand versus Soluble Agonist
One fundamental property of the TNR receptor (TNFR) family relates to how ‘signal quality’ (the extent of receptor ligation or cross-linking) influences the outcome of receptor ligation, for instance the induction of death in tumour cells. It is ...
More >