Jia-Yi Wang Graduate Institute of Medical Sciences and Department of Physiology, College of Medicine, Taipei Medical University, Taiwan
1 protocol

Ya-Ni Huang
  • Department of Nursing, Hsin Sheng Junior College of Medical Care and Management, Taiwan
Research focus
  • Neuroscience
  • 1 Author merit


Ph.D. in Neuroscience, Graduate Institute of Life Sciences, Nation Defense Medical Center, Taipei, Taiwan, 2009

Current position

Assistant professor, Department of Nursing, Hsin Sheng Junior College of Medical Care and Management, Taoyuan City, Taiwan

Publications (since 2006)

  1. Huang, Y. N., Yang, L. Y., Wang, J. Y., Lai, C. C., Chiu, C. T. and Wang, J. Y. (2016). L-Ascorbate Protects Against Methamphetamine-Induced Neurotoxicity of Cortical Cells via Inhibiting Oxidative Stress, Autophagy, and Apoptosis. Mol Neurobiol. (Epub ahead of print)
  2. Huang, Y. N., Ho, Y. J., Lai, C. C., Chiu, C. T. and Wang, J. Y. (2015). 1,25-Dihydroxyvitamin D3 attenuates endotoxin-induced production of inflammatory mediators by inhibiting MAPK activation in primary cortical neuron-glia cultures. J Neuroinflammation 12: 147.
  3. Chiu, C. T., Wen, L. L., Pao, H. P., Yang, L. Y., Huang, Y. N. and Wang, J. Y. (2015). Reparixin attenuates neuronal injury in experimental Klebsiella pneumoniae meningoencephalitis through dual effects on neuroprotection and neuroinflammation. Neuropathol Appl Neurobiol.
  4. uang, Y. N., Lai, C. C., Chiu, C. T., Lin, J. J. and Wang, J. Y. (2014). L-ascorbate attenuates the endotoxin-induced production of inflammatory mediators by inhibiting MAPK activation and NF-kappaB translocation in cortical neurons/glia Cocultures. PLoS One 9(7): e97276.
  5. Tsai, R. Y., Chou, K. Y., Shen, C. H., Chien, C. C., Tsai, W. Y., Huang, Y. N., Tao, P. L., Lin, Y. S. and Wong, C. S. (2012). Resveratrol regulates N-methyl-D-aspartate receptor expression and suppresses neuroinflammation in morphine-tolerant rats. Anesth Analg 115(4): 944-952.
  6. Huang, Y. N., Wang, J. Y., Lee, C. T., Lin, C. H., Lai, C. C. and Wang, J. Y. (2012). L-ascorbate attenuates methamphetamine neurotoxicity through enhancing the induction of endogenous heme oxygenase-1. Toxicol Appl Pharmacol 265(2): 241-252.
  7. Huang, Y. N., Tsai, R. Y., Lin, S. L., Chien, C. C., Cherng, C. H., Wu, C. T., Yeh, C. C. and Wong, C. S. (2012). Amitriptyline attenuates astrocyte activation and morphine tolerance in rats: role of the PSD-95/NR1/nNOS/PKCgamma signaling pathway. Behav Brain Res 229(2): 401-411.
  8. Xing, H. Y., Gao, H. Z., Tan, X. G., Fan, C. F., Gao, S., Sun, Y. A., Zhang, H., Zhong, X. H., Liu, H. J. and Huang, Y. N. (2009). [Incidence and prognosis of intra-and extracranial large-artery stenosis in the rural community of Beijing]. Zhonghua Liu Xing Bing Xue Za Zhi 30(8): 780-783.
  9. Lin, T. C., Huang, L. T., Huang, Y. N., Chen, G. S. and Wang, J. Y. (2009). Neonatal status epilepticus alters prefrontal-striatal circuitry and enhances methamphetamine-induced behavioral sensitization in adolescence. Epilepsy Behav 14(2): 316-323.
  10. Huang, Y. N., Wu, C. H., Lin, T. C. and Wang, J. Y. (2009). Methamphetamine induces heme oxygenase-1 expression in cortical neurons and glia to prevent its toxicity. Toxicol Appl Pharmacol 240(3): 315-326.
  11. Wen, L. L., Chiu, C. T., Huang, Y. N., Chang, C. F. and Wang, J. Y. (2007). Rapid glia expression and release of proinflammatory cytokines in experimental Klebsiella pneumoniae meningoencephalitis. Exp Neurol 205(1): 270-278.
  12. Wang, J. Y., Wen, L. L., Huang, Y. N., Chen, Y. T. and Ku, M. C. (2006). Dual effects of antioxidants in neurodegeneration: direct neuroprotection against oxidative stress and indirect protection via suppression of glia-mediated inflammation. Curr Pharm Des 12(27): 3521-3533.
  13. Tu, C. F., Su, Y. H., Huang, Y. N., Tsai, M. T., Li, L. T., Chen, Y. L., Cheng, C. J., Dai, D. F. and Yang, R. B. (2006). Localization and characterization of a novel secreted protein SCUBE1 in human platelets. Cardiovasc Res 71(3): 486-495.
1 Protocol published
Authors:  Ya-Ni Huang and Jia-Yi Wang, date: 04/20/2016, view: 8871, Q&A: 0
Primary neuron-glia cultures are commonly used in vitro model for neurobiological studies. Here, we provide a protocol for the isolation and culture of neuron-glial cells from cortical tissues of 1-day-old neonatal Sprague-Dawley pups. The ...
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