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Coreviewers
Agnès Groisillier Sorbonne Universités, University Pierre and Marie Curie
2 protocols

Alessandro Didonna University of California, San Francisco
10 protocols

André Grassmann University of Connecticut Health Center
1 protocol

Angela Corona University of Cagliari
3 protocols

Editor
Yanjie Li
  • Yale University
Research focus
  • Biochemistry
  • 0 Author merit

Education

Ph.D., Department of Chemistry, University of Toronto, Canada, 2010

Current position

Postdoc Associate, Department of Molecular Biophysics and Biochemistry, Yale University, USA

Publications (selected)

  1. Li, X.*, Li, Y.*, Arendt, C. S. and Hochstrasser, M. (2016). Distinct elements in the proteasomal β5 subunit propeptide required for autocatalytic processing and poteasome assembly. J Biol Chem 291(4): 1991-2003. (*Equal contribution)
  2. Li, Y., Tomko, R. J. Jr. and Hochstrasser, M. (2015). Proteasomes: Isolation and activity assays. Curr Protoc Cell Biol 67: 3 43 41-20.
  3. Wang, S. C., Li, Y., Ho, M., Bernal, M. E., Sydor, A. M., Kagzi, W. R. and Zamble, D. B. (2010). The response of Escherichia coli NikR to nickel: a second nickel-binding site. Biochemistry 49(31): 6635-6645.
  4. Wang, S. C., Li, Y., Robinson, C. V. and Zamble, D. B. (2010). Potassium is critical for the Ni(II)-responsive DNA-binding activity of Escherichia coli NikR. J Am Chem Soc 132(5): 1506-1507.
  5. Li, Y. and Zamble, D. B. (2009). Nickel homeostasis and nickel regulation: an overview. Chem Rev 109(10): 4617-4643.
  6. Li, Y. and Zamble, D. B. (2009). pH-responsive DNA-binding activity of Helicobacter pylori NikR. Biochemistry 48(11): 2486-2496.
5 Protocols reviewed
Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue
Authors:  Natalia V. Andreeva, Alexandra A. Dalina and Alexander V. Belyavsky, date: 08/20/2017, view: 1286, Q&A: 0
Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical ...
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Formaldehyde Fixation of Extracellular Matrix Protein Layers for Enhanced Primary Cell Growth
Authors:  Natalia V. Andreeva and Alexander V. Belyavsky, date: 07/05/2017, view: 1472, Q&A: 0
Coating tissue culture vessels with the components of the extracellular matrix such as fibronectin and collagens provides a more natural environment for primary cells in vitro and stimulates their proliferation. However, the effects of such ...
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21 Protocols edited
γ-Secretase Epsilon-cleavage Assay
γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are ...
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A Novel Protocol to Quantitatively Measure the Endocytic Trafficking of Amyloid Precursor Protein (APP) in Polarized Primary Neurons with Sub-cellular Resolution
Authors:  Florent Ubelmann, Tatiana Burrinha and Cláudia Guimas Almeida, date: 12/05/2017, view: 591, Q&A: 0
Alzheimer’s disease’s established primary trigger is β-amyloid (Aβ) (Mucke and Selkoe, 2012). The amyloid precursor protein (APP) endocytosis is required for Aβ generation at early endosomes (Rajendran and Annaert, 2012). APP retention at endosomes ...
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