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Serge Rivest
Neuroscience Laboratory, CHU de Quebec Research Center and department of Molecular Medicine, Faculty of Medicine, Laval University, Canada
2 protocols
Peter Theriault
Neuroscience Laboratory, CHU de Quebec Research Center and department of Molecular Medicine, Faculty of Medicine, Laval University, Canada
1 protocol
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Maude Bordeleau
Neuroscience Laboratory, CHU de Quebec Research Center and department of Molecular Medicine, Faculty of Medicine, Laval University, Canada
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1 Protocol published
Isolation and Purification of Murine Microglial Cells for Flow Cytometry
Authors:
Peter Theriault
,
Maude Bordeleau
and
Serge Rivest
,
date:
01/05/2016,
view:
4562,
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The detailed protocol is used to isolate different cell types from murine brain as glial cells, including microglia, using an enzymatic digestion that minimizes cellular mortality. A Percoll gradient (30% to 80%) separation allows a maximal recovery ...
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