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Coauthors
Serge Rivest Neuroscience Laboratory, CHU de Quebec Research Center and department of Molecular Medicine, Faculty of Medicine, Laval University, Canada
2 protocols

Peter Theriault Neuroscience Laboratory, CHU de Quebec Research Center and department of Molecular Medicine, Faculty of Medicine, Laval University, Canada
1 protocol

Maude Bordeleau
  • Neuroscience Laboratory, CHU de Quebec Research Center and department of Molecular Medicine, Faculty of Medicine, Laval University, Canada
  • 1 Author merit
1 Protocol published
Isolation and Purification of Murine Microglial Cells for Flow Cytometry
Authors:  Peter Theriault, Maude Bordeleau and Serge Rivest, date: 01/05/2016, view: 4160, Q&A: 0
The detailed protocol is used to isolate different cell types from murine brain as glial cells, including microglia, using an enzymatic digestion that minimizes cellular mortality. A Percoll gradient (30% to 80%) separation allows a maximal recovery ...