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Nienke Buddelmeijer
Institut Pasteur, Biology and Genetics of the Bacterial Cell Wall Unit, INSERM Group Avenir, France
2 protocols
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Sébastien Gélis-Jeanvoine
Micalis INRA, Domaine de Vilvert, France
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Microbiology
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1 Protocol published
Substituted Cysteine Accessibility Method for Topology and Activity Studies of Membrane Enzymes Forming Thioester Acyl Intermediates in Bacteria
Authors:
Sébastien Gélis-Jeanvoine
and
Nienke Buddelmeijer
,
date:
11/05/2015,
view:
7550,
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The topology of membrane proteins and enzymes can be determined using various methods including reporter protein fusions and accessibility of cysteine residues to alkylating agents. Here we describe a variation of the substituted cysteine ...
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