Reiko Motohashi Agriculture Department, Shizuoka University, Japan
3 protocols

Daisaku Ohta Graduate School of Agricultural and Biological Science, Osaka Prefecture University, Japan
1 protocol

Akira Oikawa Graduate School of Agricultural and Biological Science, Osaka Prefecture University, Japan
1 protocol

Masakazu Satou Center for Sustainable Resource Science (CSRS), RIKEN, Japan
1 protocol

Fumiyoshi Myouga
  • RIKEN Center for Sustainable Resource Science, Japan
  • 2 Author merit

Education

Ph.D. in Graduate School of Agricultural and Life Sciences, Faculty of Agriculture, The University of Tokyo, 2001

Current position

Research Scientist in Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Japan

Publications (since 2006)

  1. Nakahara, J., Takechi, K., Myouga, F., Moriyama, Y., Sato, H., Takio, S. and Takano, H. (2015). Bending of protonema cells in a plastid glycolate/glycerate transporter knockout line of Physcomitrella patens. PLoS One 10(3): e0118804.
  2. Satou, M., Enoki, H., Oikawa, A., Ohta, D., Saito, K., Hachiya, T., Sakakibara, H., Kusano, M., Fukushima, A., Saito, K., Kobayashi, M., Nagata, N., Myouga, F., Shinozaki, K. and Motohashi, R. (2014). Integrated analysis of transcriptome and metabolome of Arabidopsis albino or pale green mutants with disrupted nuclear-encoded chloroplast proteins. Plant Mol Biol 85(4-5): 411-428.
  3. Myouga, F., Akiyama, K., Tomonaga, Y., Kato, A., Sato, Y., Kobayashi, M., Nagata, N., Sakurai, T. and Shinozaki, K. (2013). The Chloroplast Function Database II: a comprehensive collection of homozygous mutants and their phenotypic/genotypic traits for nuclear-encoded chloroplast proteins. Plant Cell Physiol 54(2): e2.
  4. Yamaguchi, M., Takechi, K., Myouga, F., Imura, S., Sato, H., Takio, S., Shinozaki, K. and Takano, H. (2012). Loss of the plastid envelope protein AtLrgB causes spontaneous chlorotic cell death in Arabidopsis thaliana. Plant Cell Physiol 53(1): 125-134.
  5. Peng, L., Fukao, Y., Myouga, F., Motohashi, R., Shinozaki, K. and Shikanai, T. (2011). A chaperonin subunit with unique structures is essential for folding of a specific substrate. PLoS Biol 9(4): e1001040.
  6. Bryant, N., Lloyd, J., Sweeney, C., Myouga, F. and Meinke, D. (2011). Identification of nuclear genes encoding chloroplast-localized proteins required for embryo development in Arabidopsis. Plant Physiol 155(4): 1678-1689..
  7. Tanaka, R., Rothbart, M., Oka, S., Takabayashi, A., Takahashi, K., Shibata, M., Myouga, F., Motohashi, R., Shinozaki, K., Grimm, B. and Tanaka, A. (2010). LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis. Proc Natl Acad Sci U S A 107(38): 16721-16725.
  8. Myouga, F., Akiyama, K., Motohashi, R., Kuromori, T., Ito, T., Iizumi, H., Ryusui, R., Sakurai, T. and Shinozaki, K. (2010). The Chloroplast Function Database: a large-scale collection of Arabidopsis Ds/Spm- or T-DNA-tagged homozygous lines for nuclear-encoded chloroplast proteins, and their systematic phenotype analysis. Plant J 61(3): 529-542.
  9. Hanada, K., Kuromori, T., Myouga, F., Toyoda, T. and Shinozaki, K. (2009). Increased expression and protein divergence in duplicate genes is associated with morphological diversification. PLoS Genet 5(12): e1000781.
  10. Hanada, K., Kuromori, T., Myouga, F., Toyoda, T., Li, W. H. and Shinozaki, K. (2009). Evolutionary persistence of functional compensation by duplicate genes in Arabidopsis. Genome Biol Evol 1: 409-414.
  11. Okuda, K., Hammani, K., Tanz, S. K., Peng, L., Fukao, Y., Myouga, F., Motohashi, R., Shinozaki, K., Small, I. and Shikanai, T. (2010). The pentatricopeptide repeat protein OTP82 is required for RNA editing of plastid ndhB and ndhG transcripts. Plant J 61(2): 339-349.
  12. Umezawa, T., Sugiyama, N., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Hirayama, T. and Shinozaki, K. (2009). Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. Proc Natl Acad Sci U S A 106(41): 17588-17593.
  13. Okuda, K., Chateigner-Boutin, A. L., Nakamura, T., Delannoy, E., Sugita, M., Myouga, F., Motohashi, R., Shinozaki, K., Small, I. and Shikanai, T. (2009). Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. Plant Cell 21(1): 146-156.
  14. Myouga, F., Hosoda, C., Umezawa, T., Iizumi, H., Kuromori, T., Motohashi, R., Shono, Y., Nagata, N., Ikeuchi, M. and Shinozaki, K. (2008). A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis. Plant Cell 20(11): 3148-3162.
  15. Shimizu, H., Peng, L., Myouga, F., Motohashi, R., Shinozaki, K. and Shikanai, T. (2008). CRR23/NdhL is a subunit of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. Plant Cell Physiol 49(5): 835-842.
  16. Garcia, M., Myouga, F., Takechi, K., Sato, H., Nabeshima, K., Nagata, N., Takio, S., Shinozaki, K. and Takano, H. (2008). An Arabidopsis homolog of the bacterial peptidoglycan synthesis enzyme MurE has an essential role in chloroplast development. Plant J 53(6): 924-934.
  17. Okuda, K., Myouga, F., Motohashi, R., Shinozaki, K. and Shikanai, T. (2007). Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNA editing. Proc Natl Acad Sci U S A 104(19): 8178-8183.
  18. Motohashi, R., Yamazaki, T., Myouga, F., Ito, T., Ito, K., Satou, M., Kobayashi, M., Nagata, N., Yoshida, S., Nagashima, A., Tanaka, K., Takahashi, S. and Shinozaki, K. (2007). Chloroplast ribosome release factor 1 (AtcpRF1) is essential for chloroplast development. Plant Mol Biol 64(5): 481-497.
  19. Myouga, F., Motohashi, R., Kuromori, T., Nagata, N. and Shinozaki, K. (2006). An Arabidopsis chloroplast-targeted Hsp101 homologue, APG6, has an essential role in chloroplast development as well as heat-stress response. Plant J 48(2): 249-260.
2 Protocols published
Chlorophyll Fluorescence Measurements in Arabidopsis Plants Using a Pulse-amplitude-modulated (PAM) Fluorometer
Authors:  Reiko Motohashi and Fumiyoshi Myouga, date: 05/05/2015, view: 9056, Q&A: 0
In this protocol, to analyze PSII activity in photosynthesis, we measure the Fv/Fm (Fv=Fm ± Fo) value (Fo and Fm are the minimum and maximum values of chlorophyll fluorescence of dark-adapted leaves, respectively). Fv/Fm is a reliable marker of ...
Arabidopsis Metabolome Analysis Using Infusion ESI FT-ICR/MS
Authors:  Reiko Motohashi, Masakazu Satou, Fumiyoshi Myouga, Akira Oikawa and Daisaku Ohta, date: 05/05/2015, view: 4154, Q&A: 0
We made the method for Arabidopsis metabolome analysis based on direct-infusion Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS) (IonSpec). This method was sufficiently applied to metabolic phenotyping of Arabidopsis ...
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