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Alka Mehra Institute of Genomics and Integrative Biology
17 protocols

Daniel Kraus Würzburg University Hospital
6 protocols

Emily Cope Northern Arizona University
7 protocols

Emmanuelle Berret Ecole Polytechnique Fédérale de Lausanne
9 protocols

Editor
Antoine de Morree
  • Post-Doc, Stanford university
Research focus
  • Stem cell
  • 0 Author merit

Education

PhD, Leiden University Medical Center, 2011

11 Protocols reviewed
Generation of microRNA Sponge Library
Author:  Sebastian Herzog, date: 04/20/2018, view: 53, Q&A: 0
This protocol describes the generation and functional validation of microRNA (miRNA) sponge or decoy constructs. When expressed from a strong promoter, these transcripts can sequester specific miRNA:RISC complexes, thereby resulting in a ...
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Biochemical Isolation of Myonuclei from Mouse Skeletal Muscle Tissue
Authors:  Alicia A Cutler, Anita H Corbett and Grace K Pavlath, date: 12/20/2017, view: 1009, Q&A: 0
Skeletal muscle provides the contractile force necessary for movement, swallowing, and breathing and, consequently, is necessary for survival. Skeletal muscle cells are unique in that they are extremely large cells containing thousands of nuclei. ...
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18 Protocols edited
Isolation of Primary Human Skeletal Muscle Cells
Authors:  Janelle M. Spinazzola and Emanuela Gussoni, date: 11/05/2017, view: 1029, Q&A: 0
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic ...
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Live Imaging of Myogenesis in Indirect Flight Muscles in Drosophila
Author:  Dagan Segal, date: 07/05/2017, view: 1689, Q&A: 0
The indirect flight muscles (IFMs) are the largest muscles in the fly, making up the bulk of the adult thorax. IFMs in Drosophila are generated during pupariation by fusion of hundreds of muscle precursor cells (myoblasts) with larval ...
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