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Alka Mehra Institute of Genomics and Integrative Biology
18 protocols

Daniel Kraus Würzburg University Hospital
6 protocols

Emily Cope Northern Arizona University
7 protocols

Emmanuelle Berret Ecole Polytechnique Fédérale de Lausanne
9 protocols

Editor
Antoine de Morree
  • Post-Doc, Stanford university
Research focus
  • Stem cell
  • 0 Author merit

Education

PhD, Leiden University Medical Center, 2011

12 Protocols reviewed
Expansion of Airway Basal Cells and Generation of Polarized Epithelium
Authors:  Hannah Levardon, Lael M. Yonker, Bryan P. Hurley and Hongmei Mou, date: 06/05/2018, view: 399, Q&A: 0
Airway basal stem cells are the progenitor cells within the airway that exhibit the capacity to self-renew and give rise to multiple types of differentiated airway epithelial cells. This stem cell-derived epithelium displays organized architecture ...
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Generation of microRNA Sponge Library
Author:  Sebastian Herzog, date: 04/20/2018, view: 448, Q&A: 0
This protocol describes the generation and functional validation of microRNA (miRNA) sponge or decoy constructs. When expressed from a strong promoter, these transcripts can sequester specific miRNA:RISC complexes, thereby resulting in a ...
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19 Protocols edited
Isolation of Primary Human Skeletal Muscle Cells
Authors:  Janelle M. Spinazzola and Emanuela Gussoni, date: 11/05/2017, view: 1498, Q&A: 0
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic ...
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Transmission Electron Microscopy for Analysis of Mitochondria in Mouse Skeletal Muscle
Authors:  Joseph D. McMillan and Michael A. Eisenback, date: 05/20/2018, view: 730, Q&A: 0
Skeletal muscle is the most abundant tissue in the human body and regulates a variety of functions including locomotion and whole-body metabolism. Skeletal muscle has a plethora of mitochondria, the organelles that are essential for aerobic ...
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