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About Us
For Authors
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Submit Manuscript
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Editorial Criteria
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A peer-reviewed protocol journal. No publication fee; no access fee.
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Alka Mehra
Institute of Genomics and Integrative Biology
38 protocols
Anthony Flamier
Stem Cell and Developmental Biology Laboratory
3 protocols
Daniel Kraus
Mainz University Hospital
6 protocols
Dilip Kumar
Singapore Immunology network
1 protocol
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Editor
Antoine de Morree
Post-Doc, Stanford university
Research focus
Stem cell
Contributions
0
Author merit
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Education
PhD, Leiden University Medical Center, 2011
12 Protocols reviewed
Expansion of Airway Basal Cells and Generation of Polarized Epithelium
Authors:
Hannah Levardon
,
Lael M. Yonker
,
Bryan P. Hurley
and
Hongmei Mou
,
date:
06/05/2018,
view:
7394,
Q&A:
1
Airway basal stem cells are the progenitor cells within the airway that exhibit the capacity to self-renew and give rise to multiple types of differentiated airway epithelial cells. This stem cell-derived epithelium displays organized architecture ...
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Generation of microRNA Sponge Library
Author:
Sebastian Herzog
,
date:
04/20/2018,
view:
4129,
Q&A:
0
This protocol describes the generation and functional validation of microRNA (miRNA) sponge or decoy constructs. When expressed from a strong promoter, these transcripts can sequester specific miRNA:RISC complexes, thereby resulting in a ...
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24 Protocols edited
Fibrin Breakdown Assay
Authors:
Jiayue Ling
,
Connor M. Blair
and
George S. Baillie
,
date:
04/20/2020,
view:
1779,
Q&A:
0
Fibrinolysis is an integral part of the matrix remodeling process that contributes to tissue repair. Fibrin clots are broken down during fibrinolysis in a controlled process. Fibrin degradation products (FDPs) have also been shown to have a role in ...
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Measuring cAMP Specific Phosphodiesterase Activity: A Two-step Radioassay
Authors:
Connor M. Blair
,
Jiayue Ling
and
George S. Baillie
,
date:
04/05/2020,
view:
994,
Q&A:
0
Cyclic nucleotide degrading phosphodiesterase (PDE) enzymes are crucial to the fine tuning of cAMP signaling responses, playing a pivotal role in regulating the temporal and spatial characteristics of discrete cAMP nanodomains and hence the activity ...
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