Hi!

I have a question with regards to your use of lymphoprep to purify the dissociated tumor: I am interested in collecting Ly6G+ cells from the tumor (putative granulocytic MDSCs). The lymphoprep description from the manufacturer (in my case, StemCell) states that "differences in cell density are exploited to separate granulocytes and erythrocytes from mononuclear cells", seemingly precluding its use for my purposes. But I see a Ly6G+ population in your facs plots. Does lymphoprep behave differently if it used on whole blood compared to as you do, dissociated tumor tissue? In your experience, based on the percentages of Ly6G+ you see, do you think it would be possible to extract a workable number of cells from a tumor sample? Thank you for your input! 11/23/2016 5:00:46 AM Reply