Wanghua Gong —BIO-PROTOCOL

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Mingyong Liu Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, USA
2 protocols

Ji Wang Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, USA
2 protocols

Teizo Yoshimura Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, USA
2 protocols

Chunyan Wang Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center of Cancer Research, National Cancer Institute-Frederick, NIH, USA
2 protocols

Wanghua Gong

Basic Research Program, SAIC-Frederick, USA

Research focus

Cancer biology

2 Author merit
Reviewer merit
Editor merit

2 Protocols published

Mouse Macrophage Differentiation by Induction with Macrophage Colony-Stimulating Factor

Authors: Ying Liu, Keqiang Chen, Chunyan Wang, Wanghua Gong, Teizo Yoshimura, Ji Ming Wang and Mingyong Liu, date: 09/05/2013, view: 11430, Q&A: 0

Macrophages are differentiated from circulating blood monocytes and act as tissue-resident professional phagocytes. Macrophages function in both innate and adaptive immune systems of vertebrate animals. The cytokine macrophage colony-stimulating ...

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Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation

Authors: Ying Liu, Keqiang Chen, Chunyan Wang, Wanghua Gong, Teizo Yoshimura, Ji Ming Wang and Mingyong Liu, date: 09/05/2013, view: 38378, Q&A: 1

A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and ...

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qi Ma

医科院

My results showed that tumor cells in the middle layer and macrophages in the bottom layer.

2/22/2021 7:29:04 PM Reply

Eric Tao

上海交通大学

Hi, I'm wondering if you have a clear middle layer when using 40% and 80% Percoll, and what percentage of CD45+ cells do you usually get from subcutaneous tumor? I'm doing this experiment recently and couldn't get a clear middle layer with Ficoll. We can exchange experience if you are willing to :)

2021-04-25 19:32:00

Robert Z

Nanjing University

Have you solve the problem?

2021-03-23 04:00:13

qi Ma

医科院

I use B16 model too

2021-02-28 18:24:08

Shangcheng Yu

清华大学

not tested yet, I used B16F10 as a model.

2021-02-26 18:30:44

qi Ma

医科院

how about other cells?

2021-02-24 19:04:30

Shangcheng Yu

清华大学

tumor cell also appears in the midlayer in my experiment

2021-02-23 04:49:14