Patrick Seed Department of Molecular Genetics and Microbiology Center for Microbial Pathogenesis, Department of Pediatrics, Duke University School of Medicine, USA
2 protocols

Stacey L. Bateman
  • Department of Molecular Genetics and Microbiology Center for Microbial Pathogenesis, Department of Pediatrics, Duke University School of Medicine, USA
  • 2 Author merit
2 Protocols published
Intracellular Macrophage Infections with E. coli under Nitrosative Stress
Authors:  Stacey L. Bateman and Patrick Seed, date: 10/20/2012, view: 8913, Q&A: 0
Escherichia coli (E. coli) produces disseminated infections of the urinary tract, blood, and central nervous system where it encounters professional phagocytes such as macrophages, which utilize reactive nitrogen intermediates ...
Promoter Orientation of Prokaryotic Phase-variable Genes by PCR
Authors:  Stacey L. Bateman and Patrick Seed, date: 10/20/2012, view: 5703, Q&A: 0
One major mechanism of phase variable gene expression in prokaryotes is through inversion of the promoter element for a gene or operon. This protocol describes how to detect the promoter orientation of a phase-variable gene by PCR. This protocol, ...
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