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Takashi Okamoto
Department of Biological Sciences, Tokyo Metropolitan University
3 protocols
Yukinosuke Ohnishi
Tokyo Metropolitan University
2 protocols
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Erika Toda
Biological Sciences, Tokyo Metropolitan University, Japan
Research focus
Plant science
Contributions
2
Author merit
Reviewer merit
Editor merit
2 Protocols published
Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
Authors:
Erika Toda
and
Takashi Okamoto
,
date:
07/20/2020,
view:
641,
Q&A:
0
Polyethylene glycol calcium (PEG-Ca
2+
)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered ...
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Electro-fusion of Gametes and Subsequent Culture of Zygotes in Rice
Authors:
Erika Toda
,
Yukinosuke Ohnishi
and
Takashi Okamoto
,
date:
12/20/2016,
view:
7393,
Q&A:
0
Electro-fusion system with isolated gametes has been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, since the female gametophytes of plants are deeply embedded ...
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