Christelle Breton CERMAV-CNRS, University of Grenoble, France
1 protocol

Valerie Chazalet CERMAV-CNRS, University of Grenoble, France
1 protocol

Joana Rocha
  • CERMAV-CNRS, University of Grenoble, France
  • 1 Author merit

Education

Ph.D in Biochemistry-Structural Biology, ITQB-NOVA Macromolecular Crystallography Unit, New University of Lisbon, Portugal in 2010

Current Position

Researcher at the Molecular and Structural Glycobiology group at CERMAV-CNRS, Grenoble, France

Publications (since 2006)

  1. Rocha, J., Ciceron, F., de Sanctis, D., Lelimousin, M., Chazalet, V., Lerouxel, O. and Breton, C. (2016). Structure of Arabidopsis thaliana FUT1 Reveals a Variant of the GT-B Class Fold and Provides Insight into Xyloglucan Fucosylation. Plant Cell 28(10): 2352-2364.
  2. Rocha, J., Ciceron, F., Lerouxel, O., Breton, C. and de Sanctis, D. (2016). The galactoside 2-alpha-L-fucosyltransferase FUT1 from Arabidopsis thaliana: crystallization and experimental MAD phasing. Acta Crystallogr F Struct Biol Commun 72(Pt 7): 564-568.
  3. Ciceron, F., Rocha, J., Kousar, S., Hansen, S. F., Chazalet, V., Gillon, E., Breton, C. and Lerouxel, O. (2016). Expression, purification and biochemical characterization of AtFUT1, a xyloglucan-specific fucosyltransferase from Arabidopsis thaliana. Biochimie 128-129: 183-192.
  4. Rocha, J., Sarkis, J., Thomas, A., Pitou, L., Radzimanowski, J., Audry, M., Chazalet, V., de Sanctis, D., Palcic, M. M., Block, M. A., Girard-Egrot, A., Maréchal, E. and Breton, C. (2016). Structural insights and membrane binding properties of MGD1, the major galactolipid synthase in plants. The Plant Journal 85: 622–633.
  5. Sarkis, J., Rocha, J., Maniti, O., Jouhet, J., Vie, V., Block, M. A., Breton, C., Marechal, E. and Girard-Egrot, A. (2014). The influence of lipids on MGD1 membrane binding highlights novel mechanisms for galactolipid biosynthesis regulation in chloroplasts. FASEB J 28(7): 3114-3123.
  6. Rocha, J., Audry, M., Pesce, G., Chazalet, V., Block, M. A., Marechal, E. and Breton, C. (2013). Revisiting the expression and purification of MGD1, the major galactolipid synthase in Arabidopsis to establish a novel standard for biochemical and structural studies. Biochimie 95(4): 700-708.
  7. Rocha, J., Popescu, A. O., Borges, P., Mil-Homens, D., Moreira, L. M., Sa-Correia, I., Fialho, A. M. and Frazao, C. (2011). Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate. J Bacteriol 193(15): 3978-3987.
  8. Rocha, J., Popescu, A. O., Sa-Correia, I., Fialho, A. M. and Frazao, C. (2010). Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408. Acta Crystallogr Sect F Struct Biol Cryst Commun 66(Pt 3): 269-271.
  9. Rocha, J., Granja, A. T., Sa-Correia, I., Fialho, A. and Frazao, C. (2010). Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461. Acta Crystallogr Sect F Struct Biol Cryst Commun 66(Pt 1): 69-72.
  10. Catana, R., Eloy, M., Rocha, J. R., Ferreira, B. S., Cabral, J. M. S. and Fernandes, P. (2006). Stability evaluation of an immobilised enzyme system for inulin hydrolysis. Food Chemistry 101(1): 260-266.
  11. Rocha, J. R., Catana, R., Ferreira, B. S., Cabral, J. M. S. and Fernandes, P. (2006). Design and characterisation of an enzyme system for inulin hydrolysis. Food Chemistry 95(1): 77-82.
1 Protocol published
Expression, Purification and Crystallization of Recombinant Arabidopsis Monogalactosyldiacylglycerol Synthase (MGD1)
Authors:  Joana Rocha, Valerie Chazalet and Christelle Breton, date: 12/20/2016, view: 2938, Q&A: 0
In plant cells, galactolipids are predominant, representing up to 50% of the lipid content in photosynthetic tissues. Galactolipid synthesis is initiated by MGDG synthases (MGDs), which use UDP-galactose as a donor sugar and diacylglycerol (DAG) as ...
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