Hello, My name is Jane Lindborg and I'm a postdoc in Steve Strittmatter's lab at Yale. I've been reading through your Vad-Nielsen et al. 2016 Cell Mol Life Sci paper and I'm very interested in trying the multiplex CRISPR protocol you used. I appreciate the detail of this protocol.
I'd like to use similar plasmids so that I can express multiple gRNAs in conjunction with GFP, but I'd like to clone using a destination AAV plasmid instead of the pMsgRNA-EGFP plasmid.
I'm new to this technology, and I was wondering if it's possible to swap out the pMsgRNA-EGFP plasmid for an AAV backbone using the protocol steps outlined in your paper?
Thank you for your help!
Best,
Jane
10/28/2018 5:48:43 PM Reply