Pawan Kumar National Institute of Immunology, Aruna Asaf Ali Marg, India
1 protocol

Jay K Kolls
  • Richard King Mellon Foundation Institute for Pediatric Research, Children’s Hospital of Pittsburgh of UPMC, University of Pittsburgh, USA
  • 1 Author merit


M.D, University of Maryland

Current Position

Professor of Pediatrics and Director of the Richard King Mellon Foundation, University of Pittsburgh, Pittsburgh

Publications (since 2014)

  1. Chen, K., Campfield, B. T., Wenzel, S. E., McAleer, J. P., Kreindler, J. L., Kurland, G., Gopal, R., Wang, T., Chen, W., Eddens, T., Quinn, K. M., Myerburg, M. M., Horne, W. T., Lora, J. M., Albrecht, B. K., Pilewski, J. M. and Kolls, J. K. (2016). Antiinflammatory effects of bromodomain and extraterminal domain inhibition in cystic fibrosis lung inflammation. JCI Insight 1(11).
  2. Way, E. E., Trevejo-Nunez, G., Kane, L. P., Steiner, B. H., Puri, K. D., Kolls, J. K. and Chen, K. (2016). Dose-Dependent Suppression of Cytokine production from T cells by a Novel Phosphoinositide 3-Kinase Delta Inhibitor. Sci Rep 6: 30384.
  3. Trevejo-Nunez, G., Elsegeiny, W., Conboy, P., Chen, K. and Kolls, J. K. (2016). Critical Role of IL-22/IL22-RA1 Signaling in Pneumococcal Pneumonia. J Immunol 197(5): 1877-1883.
  4. Metz, H. E., Kargl, J., Busch, S. E., Kim, K. H., Kurland, B. F., Abberbock, S. R., Randolph-Habecker, J., Knoblaugh, S. E., Kolls, J. K., White, M. F. and Houghton, A. M. (2016). Insulin receptor substrate-1 deficiency drives a proinflammatory phenotype in KRAS mutant lung adenocarcinoma. Proc Natl Acad Sci U S A 113(31): 8795-8800.
  5. Pinilla-Vera, M., Xiong, Z., Zhao, Y., Zhao, J., Donahoe, M. P., Barge, S., Horne, W. T., Kolls, J. K., McVerry, B. J., Birukova, A., Tighe, R. M., Foster, W. M., Hollingsworth, J., Ray, A., Mallampalli, R., Ray, P. and Lee, J. S. (2016). Full Spectrum of LPS Activation in Alveolar Macrophages of Healthy Volunteers by Whole Transcriptomic Profiling. PLoS One 11(7): e0159329.
  6. McAleer, J. P., Nguyen, N. L., Chen, K., Kumar, P., Ricks, D. M., Binnie, M., Armentrout, R. A., Pociask, D. A., Hein, A., Yu, A., Vikram, A., Bibby, K., Umesaki, Y., Rivera, A., Sheppard, D., Ouyang, W., Hooper, L. V. and Kolls, J. K. (2016). Pulmonary Th17 Antifungal Immunity Is Regulated by the Gut Microbiome. J Immunol 197(1): 97-107.
  7. Eddens, T., Song, E., Ardura, M. I. and Kolls, J. K. (2016). A protracted course of Pneumocystis pneumonia in the setting of an immunosuppressed child with GMS-negative bronchoalveolar lavage. Med Mycol Case Rep 11: 48-52.
  8. Eddens, T., Campfield, B. T., Serody, K., Manni, M. L., Horne, W., Elsegeiny, W., McHugh, K. J., Pociask, D., Chen, K., Zheng, M., Alcorn, J. F., Wenzel, S. and Kolls, J. K. (2016). A Novel CD4+ T Cell-Dependent Murine Model of Pneumocystis-driven Asthma-like Pathology. Am J Respir Crit Care Med 194(7): 807-820.
  9. Kumar, P., Monin, L., Castillo, P., Elsegeiny, W., Horne, W., Eddens, T., Vikram, A., Good, M., Schoenborn, A. A., Bibby, K., Montelaro, R. C., Metzger, D. W., Gulati, A. S. and Kolls, J. K. (2016). Intestinal Interleukin-17 Receptor Signaling Mediates Reciprocal Control of the Gut Microbiota and Autoimmune Inflammation. Immunity 44(3): 659-671.
  10. Bloodworth, M. H., Newcomb, D. C., Dulek, D. E., Stier, M. T., Cephus, J. Y., Zhang, J., Goleniewska, K., Kolls, J. K. and Peebles, R. S., Jr. (2016). STAT6 Signaling Attenuates Interleukin-17-Producing gammadelta T Cells during Acute Klebsiella pneumoniae Infection. Infect Immun 84(5): 1548-1555.
  11. Zheng, M., Horne, W., McAleer, J. P., Pociask, D., Eddens, T., Good, M., Gao, B. and Kolls, J. K. (2016). Therapeutic Role of Interleukin 22 in Experimental Intra-abdominal Klebsiella pneumoniae Infection in Mice. Infect Immun 84(3): 782-789.
  12. Shankar, J., Nguyen, M. H., Crespo, M. M., Kwak, E. J., Lucas, S. K., McHugh, K. J., Mounaud, S., Alcorn, J. F., Pilewski, J. M., Shigemura, N., Kolls, J. K., Nierman, W. C. and Clancy, C. J. (2016). Looking Beyond Respiratory Cultures: Microbiome-Cytokine Signatures of Bacterial Pneumonia and Tracheobronchitis in Lung Transplant Recipients. Am J Transplant 16(6): 1766-1778.
  13. Monin, L., Griffiths, K. L., Lam, W. Y., Gopal, R., Kang, D. D., Ahmed, M., Rajamanickam, A., Cruz-Lagunas, A., Zuniga, J., Babu, S., Kolls, J. K., Mitreva, M., Rosa, B. A., Ramos-Payan, R., Morrison, T. E., Murray, P. J., Rangel-Moreno, J., Pearce, E. J. and Khader, S. A. (2015). Helminth-induced arginase-1 exacerbates lung inflammation and disease severity in tuberculosis. J Clin Invest 125(12): 4699-4713.
  14. Phinney, D. G., Di Giuseppe, M., Njah, J., Sala, E., Shiva, S., St Croix, C. M., Stolz, D. B., Watkins, S. C., Di, Y. P., Leikauf, G. D., Kolls, J., Riches, D. W., Deiuliis, G., Kaminski, N., Boregowda, S. V., McKenna, D. H. and Ortiz, L. A. (2015). Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nat Commun 6: 8472.
  15. Brehm, J. M., Man Tse, S., Croteau-Chonka, D. C., Forno, E., Litonjua, A. A., Raby, B. A., Chen, W., Yan, Q., Boutaoui, N., Acosta-Perez, E., Avila, L., Weiss, S. T., Soto-Quiros, M., Cloutier, M. M., Hu, D., Pino-Yanes, M., Wenzel, S. E., Spear, M. L., Kolls, J. K., Burchard, E. G., Canino, G. and Celedon, J. C. (2015). A Genome-Wide Association Study of Post-bronchodilator Lung Function in Children with Asthma. Am J Respir Crit Care Med 192(5): 634-637.
  16. Garg, A. V., Amatya, N., Chen, K., Cruz, J. A., Grover, P., Whibley, N., Conti, H. R., Hernandez Mir, G., Sirakova, T., Childs, E. C., Smithgall, T. E., Biswas, P. S., Kolls, J. K., McGeachy, M. J., Kolattukudy, P. E. and Gaffen, S. L. (2015). MCPIP1 Endoribonuclease Activity Negatively Regulates Interleukin-17-Mediated Signaling and Inflammation. Immunity 43(3): 475-487.
  17. Newcomb, D. C., Cephus, J. Y., Boswell, M. G., Fahrenholz, J. M., Langley, E. W., Feldman, A. S., Zhou, W., Dulek, D. E., Goleniewska, K., Woodward, K. B., Sevin, C. M., Hamilton, R. G., Kolls, J. K. and Peebles, R. S., Jr. (2015). Estrogen and progesterone decrease let-7f microRNA expression and increase IL-23/IL-23 receptor signaling and IL-17A production in patients with severe asthma. J Allergy Clin Immunol 136(4): 1025-1034 e1011.
  18. Hadebe, S., Kirstein, F., Fierens, K., Chen, K., Drummond, R. A., Vautier, S., Sajaniemi, S., Murray, G., Williams, D. L., Redelinghuys, P., Reinhart, T. A., Fallert Junecko, B. A., Kolls, J. K., Lambrecht, B. N., Brombacher, F. and Brown, G. D. (2015). Microbial Ligand Costimulation Drives Neutrophilic Steroid-Refractory Asthma. PLoS One 10(8): e0134219.
  19. Raundhal, M., Morse, C., Khare, A., Oriss, T. B., Milosevic, J., Trudeau, J., Huff, R., Pilewski, J., Holguin, F., Kolls, J., Wenzel, S., Ray, P. and Ray, A. (2015). High IFN-gamma and low SLPI mark severe asthma in mice and humans. J Clin Invest 125(8): 3037-3050.
  20. Dann, S. M., Manthey, C. F., Le, C., Miyamoto, Y., Gima, L., Abrahim, A., Cao, A. T., Hanson, E. M., Kolls, J. K., Raz, E., Cong, Y. and Eckmann, L. (2015). IL-17A promotes protective IgA responses and expression of other potential effectors against the lumen-dwelling enteric parasite Giardia. Exp Parasitol 156: 68-78.
  21. Jamal, A. J., Resende, M. R., Prochnow, T., McGilvray, I., Pilewski, J. M., Crespo, M. M., Singer, L. G., McCurry, K. R., Kolls, J. K., Keshavjee, S., Liles, W. C. and Husain, S. (2015). Simkania negevensis and acute cellular rejection in lung transplant recipients. Clin Transplant 29(8): 705-711.
  22. Ray, M., Horne, W., McAleer, J. P., Ricks, D. M., Kreindler, J. L., Fitzsimons, M. S., Chan, P. P., Trevejo-Nunez, G., Chen, K., Fajt, M., Chen, W., Ray, A., Wenzel, S. and Kolls, J. K. (2015). RNA-seq in Pulmonary Medicine: How Much Is Enough? Am J Respir Crit Care Med 192(3): 389-391.
  23. Brehm, J. M., Ramratnam, S. K., Tse, S. M., Croteau-Chonka, D. C., Pino-Yanes, M., Rosas-Salazar, C., Litonjua, A. A., Raby, B. A., Boutaoui, N., Han, Y. Y., Chen, W., Forno, E., Marsland, A. L., Nugent, N. R., Eng, C., Colon-Semidey, A., Alvarez, M., Acosta-Perez, E., Spear, M. L., Martinez, F. D., Avila, L., Weiss, S. T., Soto-Quiros, M., Ober, C., Nicolae, D. L., Barnes, K. C., Lemanske, R. F., Jr., Strunk, R. C., Liu, A., London, S. J., Gilliland, F., Sleiman, P., March, M., Hakonarson, H., Duan, Q. L., Kolls, J. K., Fritz, G. K., Hu, D., Fani, N., Stevens, J. S., Almli, L. M., Burchard, E. G., Shin, J., McQuaid, E. L., Ressler, K., Canino, G. and Celedon, J. C. (2015). Stress and Bronchodilator Response in Children with Asthma. Am J Respir Crit Care Med 192(1): 47-56.
  24. Nguyen, N. L., Pilewski, J. M., Celedon, J. C., Mandalapu, S., Blanchard, M. L., DeRicco, A., Hartigan, E., Alcorn, J. F. and Kolls, J. K. (2015). Vitamin D supplementation decreases Aspergillus fumigatus specific Th2 responses in CF patients with aspergillus sensitization: a phase one open-label study. Asthma Res Pract 1.
  25. Elsegeiny, W., Eddens, T., Chen, K. and Kolls, J. K. (2015). Anti-CD20 antibody therapy and susceptibility to Pneumocystis pneumonia. Infect Immun 83(5): 2043-2052.
  26. Trevejo-Nunez, G., Chen, K., Dufour, J. P., Bagby, G. J., Horne, W. T., Nelson, S. and Kolls, J. K. (2015). Ethanol impairs mucosal immunity against Streptococcus pneumoniae infection by disrupting interleukin 17 gene expression. Infect Immun 83(5): 2082-2088.
  27. Robinson, K. M., Lee, B., Scheller, E. V., Mandalapu, S., Enelow, R. I., Kolls, J. K. and Alcorn, J. F. (2015). The role of IL-27 in susceptibility to post-influenza Staphylococcus aureus pneumonia. Respir Res 16: 10.
  28. Vautier, S., Drummond, R. A., Chen, K., Murray, G. I., Kadosh, D., Brown, A. J., Gow, N. A., MacCallum, D. M., Kolls, J. K. and Brown, G. D. (2015). Candida albicans colonization and dissemination from the murine gastrointestinal tract: the influence of morphology and Th17 immunity. Cell Microbiol 17(4): 445-450.
  29. Fang, P., Zhou, L., Zhou, Y., Kolls, J. K., Zheng, T. and Zhu, Z. (2014). Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation. PLoS One 9(9): e107454.
  30. Xu, M. J., Feng, D., Wu, H., Wang, H., Chan, Y., Kolls, J., Borregaard, N., Porse, B., Berger, T., Mak, T. W., Cowland, J. B., Kong, X. and Gao, B. (2015). Liver is the major source of elevated serum lipocalin-2 levels after bacterial infection or partial hepatectomy: a critical role for IL-6/STAT3. Hepatology 61(2): 692-702.
  31. Shih, V. F., Cox, J., Kljavin, N. M., Dengler, H. S., Reichelt, M., Kumar, P., Rangell, L., Kolls, J. K., Diehl, L., Ouyang, W. and Ghilardi, N. (2014). Homeostatic IL-23 receptor signaling limits Th17 response through IL-22-mediated containment of commensal microbiota. Proc Natl Acad Sci U S A 111(38): 13942-13947.
  32. Adams, K. M., Abraham, V., Spielman, D., Kolls, J. K., Rubenstein, R. C., Conner, G. E., Cohen, N. A. and Kreindler, J. L. (2014). IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells. PLoS One 9(8): e103263.
  33. Dulek, D. E., Newcomb, D. C., Goleniewska, K., Cephus, J., Zhou, W., Reiss, S., Toki, S., Ye, F., Zaynagetdinov, R., Sherrill, T. P., Blackwell, T. S., Moore, M. L., Boyd, K. L., Kolls, J. K. and Peebles, R. S., Jr. (2014). Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner. Infect Immun 82(9): 3723-3739.
  34. Xu, B., Guenther, J. F., Pociask, D. A., Wang, Y., Kolls, J. K., You, Z., Chandrasekar, B., Shan, B., Sullivan, D. E. and Morris, G. F. (2014). Promotion of lung tumor growth by interleukin-17. Am J Physiol Lung Cell Mol Physiol 307(6): L497-508.
  35. Adams, K. M., Abraham, V., Spielman, D., Kolls, J. K., Rubenstein, R. C., Conner, G. E., Cohen, N. A. and Kreindler, J. L. (2014). IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells. PLoS One 9(8): e103263.
  36. Gopal, R., Monin, L., Slight, S., Uche, U., Blanchard, E., Fallert Junecko, B. A., Ramos-Payan, R., Stallings, C. L., Reinhart, T. A., Kolls, J. K., Kaushal, D., Nagarajan, U., Rangel-Moreno, J. and Khader, S. A. (2014). Unexpected role for IL-17 in protective immunity against hypervirulent Mycobacterium tuberculosis HN878 infection. PLoS Pathog 10(5): e1004099.
  37. McAllister, F., Bailey, J. M., Alsina, J., Nirschl, C. J., Sharma, R., Fan, H., Rattigan, Y., Roeser, J. C., Lankapalli, R. H., Zhang, H., Jaffee, E. M., Drake, C. G., Housseau, F., Maitra, A., Kolls, J. K., Sears, C. L., Pardoll, D. M. and Leach, S. D. (2014). Oncogenic Kras activates a hematopoietic-to-epithelial IL-17 signaling axis in preinvasive pancreatic neoplasia. Cancer Cell 25(5): 621-637.
  38. Deshmukh, H. S., Liu, Y., Menkiti, O. R., Mei, J., Dai, N., O'Leary, C. E., Oliver, P. M., Kolls, J. K., Weiser, J. N. and Worthen, G. S. (2014). The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice. Nat Med 20(5): 524-530.
  39. Zheng, M., Cai, Y., Eddens, T., Ricks, D. M. and Kolls, J. K. (2014). Novel pneumocystis antigen discovery using fungal surface proteomics. Infect Immun 82(6): 2417-2423.
  40. Robinson, K. M., McHugh, K. J., Mandalapu, S., Clay, M. E., Lee, B., Scheller, E. V., Enelow, R. I., Chan, Y. R., Kolls, J. K. and Alcorn, J. F. (2014). Influenza A virus exacerbates Staphylococcus aureus pneumonia in mice by attenuating antimicrobial peptide production. J Infect Dis 209(6): 865-875.
  41. Manni, M. L., Trudeau, J. B., Scheller, E. V., Mandalapu, S., Elloso, M. M., Kolls, J. K., Wenzel, S. E. and Alcorn, J. F. (2014). The complex relationship between inflammation and lung function in severe asthma. Mucosal Immunol 7(5): 1186-1198.
  42. Gopal, R., Rangel-Moreno, J., Fallert Junecko, B. A., Mallon, D. J., Chen, K., Pociask, D. A., Connell, T. D., Reinhart, T. A., Alcorn, J. F., Ross, T. M., Kolls, J. K. and Khader, S. A. (2014). Mucosal pre-exposure to Th17-inducing adjuvants exacerbates pathology after influenza infection. Am J Pathol 184(1): 55-63.
  43. Choi, S. M., McAleer, J. P., Trevejo-Nunez, G., Pociask, D. A., Armentrout, R. A., Chen, K. and Kolls, J. K. (2014). Acute alcohol intoxication impairs methicillin-resistant Staphylococcus aureus clearance in the lung by impeding epithelial production of Reg3gamma. Infect Immun 82(4): 1402-1407.
1 Protocol published
Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining
Authors:  Pawan Kumar and Jay K Kolls, date: 12/05/2016, view: 6033, Q&A: 0
In vitro Th17 (α, β T helper cell which produce IL-17A, IL-17F and IL-22) differentiation has been routinely used for functional T cells studies. Here we describe a method for Th17 cell differentiation.
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