Fergal O’Gara Biomerit Research Centre, School of Microbiology, University College Cork, Ireland
1 protocol

F Jerry Reen Biomerit Research Centre, School of Microbiology, University College Cork, Ireland
1 protocol
David Paul MRC Laboratory of Molecular Biology
41 protocols

Juan Facundo Ayala Instituto de Nanociencia y Nanotecnología
25 protocols

John P Phelan
  • Research scientist, University College Cork
Research focus
  • Molecular biology
  • (1) Microbial-Host Interactions, (2) Functional Genomics and Signalling in Gram-negative bacteria and (3) Environmental & Marine Biotechnology.
  • 1 Author merit


PhD, University College London, 2005

Lab information


1 Protocol published
Determining the Influence of Small Molecules on Hypoxic Prostate Cancer Cell (DU-145) Viability Using Automated Cell Counting and a Cell Harvesting Protocol
Authors:  John P Phelan, F Jerry Reen and Fergal O’Gara, date: 11/20/2016, view: 5898, Q&A: 0
Cell viability assays are an essential aspect of most cancer studies, however they usually require a considerable labor and time input. Here, instead of using the conventional microscopy and hemocytometer cell counting approach, we developed a cell ...
2 Protocols reviewed
Analysis of TORC1-body Formation in Budding Yeast
Authors:  Ryan L. Wallace, Eric Lu, Arron Sullivan, James E. Hughes Hallett and Andrew P. Capaldi, date: 04/05/2021, view: 1797, Q&A: 0

The Target of Rapamycin kinase Complex I (TORC1) is the master regulator of cell growth and metabolism in eukaryotes. In the presence of pro-growth hormones and abundant nutrients, TORC1 is active and drives protein, lipid, and nucleotide synthesis

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Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein
Authors:  Sayan Dutta, Ben G. Watson, Seema Mattoo and Jean-Christophe Rochet, date: 07/20/2020, view: 2586, Q&A: 0
Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on ...
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