Hi there, thank you for the very detailed protocol. I am having moderate success with perfusion and clearing the RBC from the lungs, sometimes it works perfectly but I have had instances where there is mottling and some areas of the lungs remain very dark. Any advice on this? Should I increase volume of PBS until completely cleared? How does one know how deep they are in the right ventricle?
Appreciate any tips you could share.
Kind regards,
Caroline
9/10/2020 1:40:58 AM Reply