Clifford Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Andrea Puhar The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
44 protocols

Antoine de Morree Stanford university
33 protocols

Benoit Chassaing Georgia State University
14 protocols

Editor
Meenal Sinha
  • Research scientist, University of California San Francisco
Research focus
  • Immunology
  • 1 Author merit

Education

Ph.D., University of Texas at Houston, 2008

1 Protocol published
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:  Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 12843, Q&A: 0
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
18 Protocols reviewed
Induced Germinal Center B Cell Culture System
Authors:  Kei Haniuda and Daisuke Kitamura, date: 02/20/2019, view: 2289, Q&A: 0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
Authors:  Michael S. Schappe and Bimal N. Desai, date: 07/20/2018, view: 3138, Q&A: 0
After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan et ...
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4 Protocols edited
Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
Authors:  Agnieszka P. Looney and Mallar Bhattacharya, date: 08/20/2019, view: 350, Q&A: 0
Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

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Precision Technique for Splenectomy Limits Mouse Stress Responses for Accurate and Realistic Measurements for Investigating Inflammation and Immunity
Authors:  Shengwen Calvin Li, Anthony D. Rangel and Mustafa H. Kabeer, date: 08/05/2019, view: 361, Q&A: 0
Splenectomy in an animal model requires a standardized technique utilizing best practice to avoid variability which can result in adverse impact to the animal resulting in flawed physiologic responses simply due to technique rather than to the ...
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