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A peer-reviewed protocol journal. No publication fee; no access fee.
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Clifford Lowell
Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
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Alesssandro Arduini
BROAD Institute of Harvard and MIT
2 protocols
Andrea Puhar
The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
47 protocols
Anita Umesh
Illumina
4 protocols
Antoine de Morree
Stanford university
36 protocols
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Editor
Meenal Sinha
Research scientist, University of California San Francisco
Research focus
Immunology
Contributions
1
Author merit
Reviewer merit
Editor merit
Education
Ph.D., University of Texas at Houston, 2008
1 Protocol published
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:
Meenal Sinha
and
Clifford A. Lowell
,
date:
11/20/2016,
view:
15775,
Q&A:
0
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
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18 Protocols reviewed
Induced Germinal Center B Cell Culture System
Authors:
Kei Haniuda
and
Daisuke Kitamura
,
date:
02/20/2019,
view:
4821,
Q&A:
0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
Authors:
Michael S. Schappe
and
Bimal N. Desai
,
date:
07/20/2018,
view:
4785,
Q&A:
0
After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan
et
...
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11 Protocols edited
Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from
Mycobacterium tuberculosis
-infected Mice
Authors:
Alissa C. Rothchild
,
Dat Mai
,
Alan Aderem
and
Alan H. Diercks
,
date:
05/20/2020,
view:
600,
Q&A:
0
Mycobacterium tuberculosis
(Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing ...
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Evaluation of B Cell Proliferation
in vivo
by EdU Incorporation Assay
Authors:
Adi Biram
and
Ziv Shulman
,
date:
05/05/2020,
view:
579,
Q&A:
0
Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens ...
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