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A peer-reviewed protocol journal. No publication fee; no access fee.
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Clifford Lowell
Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol
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Alesssandro Arduini
BROAD Institute of Harvard and MIT
2 protocols
Andrea Puhar
The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
44 protocols
Antoine de Morree
Stanford university
33 protocols
Benoit Chassaing
Georgia State University
14 protocols
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Editor
Meenal Sinha
Research scientist, University of California San Francisco
Research focus
Immunology
Contributions
1
Author merit
Reviewer merit
Editor merit
Education
Ph.D., University of Texas at Houston, 2008
1 Protocol published
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:
Meenal Sinha
and
Clifford A. Lowell
,
date:
11/20/2016,
view:
12843,
Q&A:
0
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
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18 Protocols reviewed
Induced Germinal Center B Cell Culture System
Authors:
Kei Haniuda
and
Daisuke Kitamura
,
date:
02/20/2019,
view:
2289,
Q&A:
0
The germinal center (GC) is the site where B cells undergo clonal expansion, affinity-based selection, and differentiation into memory B cells or plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell ...
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Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
Authors:
Michael S. Schappe
and
Bimal N. Desai
,
date:
07/20/2018,
view:
3138,
Q&A:
0
After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan
et
...
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4 Protocols edited
Fibroblast Gap-closure Assay-Microscopy-based
in vitro
Assay Measuring the Migration of Murine Fibroblasts
Authors:
Agnieszka P. Looney
and
Mallar Bhattacharya
,
date:
08/20/2019,
view:
350,
Q&A:
0
Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.
...
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Precision Technique for Splenectomy Limits Mouse Stress Responses for Accurate and Realistic Measurements for Investigating Inflammation and Immunity
Authors:
Shengwen Calvin Li
,
Anthony D. Rangel
and
Mustafa H. Kabeer
,
date:
08/05/2019,
view:
361,
Q&A:
0
Splenectomy in an animal model requires a standardized technique utilizing best practice to avoid variability which can result in adverse impact to the animal resulting in flawed physiologic responses simply due to technique rather than to the ...
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