Meenal Sinha University of California San Francisco

Home

Archive

About Us

Clifford Lowell Department of Laboratory Medicine and the Program in Immunology, University of California, USA
1 protocol

Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Alka Mehra Institute of Genomics and Integrative Biology
44 protocols

Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
57 protocols

Anita Umesh Illumina
4 protocols

Editor

Meenal Sinha

Research scientist, University of California San Francisco

Research focus

Immunology

1 Author merit
Reviewer merit
Editor merit

Education

Ph.D., University of Texas at Houston, 2008

1 Protocol published

Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting

Authors: Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 22535, Q&A: 1

In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...

More >

20 Protocols reviewed

Production, Titration, Neutralisation, Storage and Lyophilisation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Lentiviral Pseudotypes

Authors: Cecilia Di Genova, Alex Sampson, Simon Scott, Diego Cantoni, Martin Mayora-Neto, Emma Bentley, Giada Mattiuzzo, Edward Wright, Mariliza Derveni, Bethany Auld, Bill T. Ferrara, Dale Harrison, Mohamed Said, Arwa Selim, Elinor Thompson, Craig Thompson, George Carnell and Nigel Temperton, date: 11/05/2021, view: 1954, Q&A: 0

This protocol details a rapid and reliable method for the production and titration of high-titre viral pseudotype particles with the SARS-CoV-2 spike protein (and D614G or other variants of concern, VOC) on a lentiviral vector core, and use for

...

More >

Isolation of Microglia and Analysis of Protein Expression by Flow Cytometry: Avoiding the Pitfall of Microglia Background Autofluorescence

Authors: Jeremy C. Burns, Richard M. Ransohoff and Michaël Mingueneau, date: 07/20/2021, view: 2857, Q&A: 0

Microglia are a unique type of tissue-resident innate immune cell found within the brain, spinal cord, and retina. In the healthy nervous system, their main functions are to defend the tissue against infectious microbes, support neuronal networks

...

More >

More protocols >

11 Protocols edited

Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice

Authors: Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks, date: 05/20/2020, view: 3971, Q&A: 0

Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing ...

More >

Evaluation of B Cell Proliferation in vivo by EdU Incorporation Assay

Authors: Adi Biram and Ziv Shulman, date: 05/05/2020, view: 3865, Q&A: 0

Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens ...

More >

More protocols >

Caroline Beltran

Stellenbosh University