Lingling Zhang School of Optometry, Indiana University, USA
1 protocol

Okada Yuka School of Optometry, Indiana University, USA
1 protocol

Yen-Chiao Wang School of Optometry, Indiana University, USA
1 protocol

Yujin Zhang School of Optometry, Indiana University, USA
1 protocol

Chia-Yang Liu
  • School of Optometry, Indiana University, USA
Research focus
  • Cell biology
  • 1 Author merit


Ph.D., University of Cincinnati Medical Center (Interdisciplinary Ph.D. Program/Molecular Ophthalmology), 1993

Current position

Associate Professor, School of Optometry, Indiana University at Bloomington

Publications (since 2010)

  1. Liu, C. Y. (2015). Wakayama symposium: role of canonical Notch signaling in conjucntival goblet cell differentiation and dry eye syndrome. BMC Ophthalmol 15 Suppl 1: 152.

  2. Dong, F., Liu, C. Y., Yuan, Y., Zhang, Y., Li, W., Call, M., Zhang, L., Chen, Y., Liu, Z. and Kao, W. W. (2015). Perturbed meibomian gland and tarsal plate morphogenesis by excess TGFalpha in eyelid stroma. Dev Biol 406(2): 147-157.

  3. Zhang, Y., Yeh, L. K., Zhang, S., Call, M., Yuan, Y., Yasunaga, M., Kao, W. W. and Liu, C. Y. (2015). Wnt/beta-catenin signaling modulates corneal epithelium stratification via inhibition of Bmp4 during mouse development. Development 142(19): 3383-3393.Coulson-Thomas, V. J., Chang, S. H., Yeh, L. K., Coulson-Thomas, Y. M., Yamaguchi, Y., Esko, J., Liu, C. Y. and Kao, W. (2015). Loss of corneal epithelial heparan sulfate leads to corneal degeneration and impaired wound healing. Invest Ophthalmol Vis Sci 56(5): 3004-3014.

  4. Mizoguchi, S., Suzuki, K., Zhang, J., Yamanaka, O., Liu, C. Y., Okada, Y., Miyajima, M., Kokado, M., Kao, W. W., Yamada, G. and Saika, S. (2015). Disruption of eyelid and cornea morphogenesis by epithelial beta-catenin gain-of-function. Mol Vis 21: 793-803.

  5. Liu, C. Y. and Kao, W. W. (2015). Corneal Epithelial Wound Healing. Prog Mol Biol Transl Sci 134: 61-71.

  6. Byun, Y. S., Tibrewal, S., Kim, E., Yco, L., Sarkar, J., Ivanir, Y., Liu, C. Y., Sano, C. M. and Jain, S. (2014). Keratocytes derived from spheroid culture of corneal stromal cells resemble tissue resident keratocytes. PLoS One 9(11): e112781.

  7. Yang, D., Sun, Y. Y., Bhaumik, S. K., Li, Y., Baumann, J. M., Lin, X., Zhang, Y., Lin, S. H., Dunn, R. S., Liu, C. Y., Shie, F. S., Lee, Y. H., Wills-Karp, M., Chougnet, C. A., Kallapur, S. G., Lewkowich, I. P., Lindquist, D. M., Murali-Krishna, K. and Kuan, C. Y. (2014). Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns. J Neurosci 34(49): 16467-16481.

  8. Meng, Q., Mongan, M., Carreira, V., Kurita, H., Liu, C. Y., Kao, W. W. and Xia, Y. (2014). Eyelid closure in embryogenesis is required for ocular adnexa development. Invest Ophthalmol Vis Sci 55(11): 7652-7661.

  9. McCauley, H. A., Liu, C. Y., Attia, A. C., Wikenheiser-Brokamp, K. A., Zhang, Y., Whitsett, J. A. and Guasch, G. (2014). TGFbeta signaling inhibits goblet cell differentiation via SPDEF in conjunctival epithelium. Development 141(23): 4628-4639.

  10. Feng, L., Li, S. K., Liu, H., Liu, C. Y., LaSance, K., Haque, F., Shu, D. and Guo, P. (2014). Ocular delivery of pRNA nanoparticles: distribution and clearance after subconjunctival injection. Pharm Res 31(4): 1046-1058.

  11. Wu, Y. C., Chen, R. F., Liu, C. Y., Hu, F. R., Huang, C. J. and Wang, I. J. (2014). Knockdown of zebrafish blood vessel epicardial substance results in incomplete retinal lamination. ScientificWorldJournal 2014: 803718.

  12. Yamanaka, O., Yuan, Y., Coulson-Thomas, V. J., Gesteira, T. F., Call, M. K., Zhang, Y., Zhang, J., Chang, S. H., Xie, C., Liu, C. Y., Saika, S., Jester, J. V. and Kao, W. W. (2013). Lumican binds ALK5 to promote epithelium wound healing. PLoS One 8(12): e82730.

  13. Ng, G. Y., Yeh, L. K., Zhang, Y., Liu, H., Feng, G. S., Kao, W. W. and Liu, C. Y. (2013). Role of SH2-containing tyrosine phosphatase Shp2 in mouse corneal epithelial stratification. Invest Ophthalmol Vis Sci 54(13): 7933-7942.

  14. Yuan, Y., Call, M. K., Yuan, Y., Zhang, Y., Fischesser, K., Liu, C. Y. and Kao, W. W. (2013). Dexamethasone induces cross-linked actin networks in trabecular meshwork cells through noncanonical wnt signaling. Invest Ophthalmol Vis Sci 54(10): 6502-6509.

  15. Yuan, Y., Yeh, L. K., Liu, H., Yamanaka, O., Hardie, W. D., Kao, W. W. and Liu, C. Y. (2013). Targeted overexpression of TGF-alpha in the corneal epithelium of adult transgenic mice induces changes in anterior segment morphology and activates noncanonical Wnt signaling. Invest Ophthalmol Vis Sci 54(3): 1829-1837.

  16. Zhang, Y., Lam, O., Nguyen, M. T., Ng, G., Pear, W. S., Ai, W., Wang, I. J., Kao, W. W. and Liu, C. Y. (2013). Mastermind-like transcriptional co-activator-mediated Notch signaling is indispensable for maintaining conjunctival epithelial identity. Development 140(3): 594-605.

  17. Liu, C. Y. (2012). Wakayama Symposium: Notch-FoxL2-alpha-SMA axis in eyelid levator muscle development and congenital blepharophimosis. Ocul Surf 10(4): 221-223.

  18. Wu, Y. C., Liu, C. Y., Chen, Y. H., Chen, R. F., Huang, C. J. and Wang, I. J. (2012). Blood vessel epicardial substance (Bves) regulates epidermal tight junction integrity through atypical protein kinase C. J Biol Chem 287(47): 39887-39897.

  19. Liu, C. Y. and Kao, W. W. (2012). Lumican promotes corneal epithelial wound healing. Methods Mol Biol 836: 285-290.

  20. Liu, H., Zhang, J., Liu, C. Y., Hayashi, Y. and Kao, W. W. (2012). Bone marrow mesenchymal stem cells can differentiate and assume corneal keratocyte phenotype. J Cell Mol Med 16(5): 1114-1124.

  21. Terai, K., Call, M. K., Liu, H., Saika, S., Liu, C. Y., Hayashi, Y., Chikama, T., Zhang, J., Terai, N., Kao, C. W. and Kao, W. W. (2011). Crosstalk between TGF-beta and MAPK signaling during corneal wound healing. Invest Ophthalmol Vis Sci 52(11): 8208-8215.

  22. Zhang, Y., Kao, W. W., Pelosi, E., Schlessinger, D. and Liu, C. Y. (2011). Notch gain of function in mouse periocular mesenchyme downregulates FoxL2 and impairs eyelid levator muscle formation, leading to congenital blepharophimosis. J Cell Sci 124(Pt 15): 2561-2572.

  23. Yeh, J. T., Yeh, L. K., Jung, S. M., Chang, T. J., Wu, H. H., Shiu, T. F., Liu, C. Y., Kao, W. W. and Chu, P. H. (2010). Impaired skin wound healing in lumican-null mice. Br J Dermatol 163(6): 1174-1180.

  24. Nien, C. J., Massei, S., Lin, G., Liu, H., Paugh, J. R., Liu, C. Y., Kao, W. W., Brown, D. J. and Jester, J. V. (2010). The development of meibomian glands in mice. Mol Vis 16: 1132-1140.

  25. Hayashi, Y., Call, M. K., Liu, C. Y., Hayashi, M., Babcock, G., Ohashi, Y. and Kao, W. W. (2010). Monoallelic expression of Krt12 gene during corneal-type epithelium differentiation of limbal stem cells. Invest Ophthalmol Vis Sci 51(9): 4562-4568.

  26. Hayashi, Y., Call, M. K., Chikama, T., Liu, H., Carlson, E. C., Sun, Y., Pearlman, E., Funderburgh, J. L., Babcock, G., Liu, C. Y., Ohashi, Y. and Kao, W. W. (2010). Lumican is required for neutrophil extravasation following corneal injury and wound healing. J Cell Sci 123(Pt 17): 2987-2995.

  27. Wang, I. J., Jhuang, M. C., Chen, Y. H., Yeh, L. K., Liu, C. Y. and Young, T. H. (2010). Chitosan modification of adenovirus to modify transfection efficiency in bovine corneal epithelial cells. PLoS One 5(8): e12085.

  28. Carlson, E. C., Sun, Y., Auletta, J., Kao, W. W., Liu, C. Y., Perez, V. L. and Pearlman, E. (2010). Regulation of corneal inflammation by neutrophil-dependent cleavage of keratan sulfate proteoglycans as a model for breakdown of the chemokine gradient. J Leukoc Biol 88(3): 517-522.

  29. Liu, H., Zhang, J., Liu, C. Y., Wang, I. J., Sieber, M., Chang, J., Jester, J. V. and Kao, W. W. (2010). Cell therapy of congenital corneal diseases with umbilical mesenchymal stem cells: lumican null mice. PLoS One 5(5): e10707.

  30. Yeh, L. K., Liu, C. Y., Kao, W. W., Huang, C. J., Hu, F. R., Chien, C. L. and Wang, I. J. (2010). Knockdown of zebrafish lumican gene (zlum) causes scleral thinning and increased size of scleral coats. J Biol Chem 285(36): 28141-28155.

  31. Tong, Y. C., Chang, S. F., Kao, W. W., Liu, C. Y. and Liaw, J. (2010). Polymeric micelle gene delivery of bcl-xL via eye drop reduced corneal apoptosis following epithelial debridement. J Control Release 147(1): 76-83.

  32. Zhang, Y., Call, M. K., Yeh, L. K., Liu, H., Kochel, T., Wang, I. J., Chu, P. H., Taketo, M. M., Jester, J. V., Kao, W. W. and Liu, C. Y. (2010). Aberrant expression of a beta-catenin gain-of-function mutant induces hyperplastic transformation in the mouse cornea. J Cell Sci 123(Pt 8): 1285-1294.

1 Protocol published
Mouse Corneal Stroma Fibroblast Primary Cell Culture
Authors:  Yujin Zhang, Yen-Chiao Wang, Okada Yuka, Lingling Zhang and Chia-Yang Liu, date: 10/05/2016, view: 5705, Q&A: 0
This protocol is developed for primary cell culture of cornea stromal keratocytes isolated from neonatal mouse eyeballs. It provides an optimal condition to isolate stromal keratocytes which maintain high viability for cell culture.
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