Christian Stehlik Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, USA
2 protocols

Lucia de Almeida Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, USA
2 protocols

Andrea Dorfleutner
  • Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, USA
  • 2 Author merit

Education

Ph.D. in Molecular Biology, University of Vienna, Austria, 2003

Current position

Research Associate Professor of Medicine, Department of Medicine/Division of Rheumatology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA

Publications

  1. Dorfleutner, A. and Stehlik, C. (2016). A dRAStic RHOAdblock of Pyrin inflammasome activation. Nat Immunol 17(8): 900-902.

  2. Ratsimandresy, R., Indramohan, M., Dorfleutner, A. and Stehlik, C. (2016) The AIM2 inflammasome is a central regulator of intestinal homeostasis through the IL-18/IL-22/STAT3 pathway. Cellular & Molecular Immunology.
  3. Khare, S., Radian, A. D., Dorfleutner, A. and Stehlik, C. (2016). Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking. Methods Mol Biol 1417: 131-143.
  4. Cuda, C. M., Misharin, A. V., Khare, S., Saber, R., Tsai, F., Archer, A. M., Homan, P. J., Haines, G. K., 3rd, Hutcheson, J., Dorfleutner, A., Budinger, G. R., Stehlik, C. and Perlman, H. (2015). Conditional deletion of caspase-8 in macrophages alters macrophage activation in a RIPK-dependent manner. Arthritis Res Ther 17: 291.
  5. Radian, A. D., Khare, S., Chu, L. H., Dorfleutner, A. and Stehlik, C. (2015). ATP binding by NLRP7 is required for inflammasome activation in response to bacterial lipopeptides. Mol Immunol 67(2 Pt B): 294-302.
  6. de Almeida, L., Khare, S., Misharin, A. V., Patel, R., Ratsimandresy, R. A., Wallin, M. C., Perlman, H., Greaves, D. R., Hoffman, H. M., Dorfleutner, A. and Stehlik, C. (2015). The PYRIN Domain-only Protein POP1 Inhibits Inflammasome Assembly and Ameliorates Inflammatory Disease. Immunity 43(2): 264-276.
  7. Dorfleutner, A., Chu, L. and Stehlik, C. (2015). Inhibiting the inflammasome: one domain at a time. Immunol Rev 265(1): 205-216.
  8. Chu, L. H., Gangopadhyay, A., Dorfleutner, A. and Stehlik, C. (2015). An updated view on the structure and function of PYRIN domains. Apoptosis 20(2): 157-173.
  9. Khare, S., Ratsimandresy, R. A., de Almeida, L., Cuda, C. M., Rellick, S. L., Misharin, A. V., Wallin, M. C., Gangopadhyay, A., Forte, E., Gottwein, E., Perlman, H., Reed, J. C., Greaves, D. R., Dorfleutner, A. and Stehlik, C. (2014). The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses. Nat Immunol 15(4): 343-353.
  10. Ratsimandresy, R. A., Dorfleutner, A. and Stehlik, C. (2013). An Update on PYRIN Domain-Containing Pattern Recognition Receptors: From Immunity to Pathology. Front Immunol 4: 440.
  11. Radian, A. D., de Almeida, L., Dorfleutner, A. and Stehlik, C. (2013). NLRP7 and related inflammasome activating pattern recognition receptors and their function in host defense and disease. Microbes Infect 15(8-9): 630-639.
  12. Khare, S., Dorfleutner, A., Bryan, N. B., Yun, C., Radian, A. D., de Almeida, L., Rojanasakul, Y. and Stehlik, C. (2012). An NLRP7-containing inflammasome mediates recognition of microbial lipopeptides in human macrophages. Immunity 36(3): 464-476.
  13. Huang, Q. Q., Koessler, R. E., Birkett, R., Dorfleutner, A., Perlman, H., Haines, G. K., 3rd, Stehlik, C., Nicchitta, C. V. and Pope, R. M. (2012). Glycoprotein 96 perpetuates the persistent inflammation of rheumatoid arthritis. Arthritis Rheum 64(11): 3638-3648.
  14. Shi, B., Huang, Q., Tak, P. P., Vervoordeldonk, M. J., Huang, C. C., Dorfleutner, A., Stehlik, C. and Pope, R. M. (2012). SNAPIN: an endogenous Toll-like receptor ligand in rheumatoid arthritis. Ann Rheum Dis 71(8): 1411-1417.
  15. Khare, S., Luc, N., Dorfleutner, A. and Stehlik, C. (2010). Inflammasomes and their activation. Crit Rev Immunol 30(5): 463-487.
  16. Bryan, N. B., Dorfleutner, A., Kramer, S. J., Yun, C., Rojanasakul, Y. and Stehlik, C. (2010). Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond) 7: 23.
  17. Shahrara, S., Pickens, S. R., Dorfleutner, A. and Pope, R. M. (2009). IL-17 induces monocyte migration in rheumatoid arthritis. J Immunol 182(6): 3884-3891.
  18. Bryan, N. B., Dorfleutner, A., Rojanasakul, Y. and Stehlik, C. (2009). Activation of inflammasomes requires intracellular redistribution of the apoptotic speck-like protein containing a caspase recruitment domain. J Immunol 182(5): 3173-3182.
  19. Dorfleutner, A., Cho, Y., Vincent, D., Cunnick, J., Lin, H., Weed, S. A., Stehlik, C. and Flynn, D. C. (2008). Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells. J Cell Sci 121(Pt 14): 2394-2405.
  20. Stehlik, C. and Dorfleutner, A. (2007). COPs and POPs: modulators of inflammasome activity. J Immunol 179(12): 7993-7998.
  21. Zhang, J., Park, S. I., Artime, M. C., Summy, J. M., Shah, A. N., Bomser, J. A., Dorfleutner, A., Flynn, D. C. and Gallick, G. E. (2007). AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts. J Clin Invest 117(10): 2962-2973.
  22. Dorfleutner, A., Talbott, S. J., Bryan, N. B., Funya, K. N., Rellick, S. L., Reed, J. C., Shi, X., Rojanasakul, Y., Flynn, D. C. and Stehlik, C. (2007). A Shope Fibroma virus PYRIN-only protein modulates the host immune response. Virus Genes 35(3): 685-694.
  23. Dorfleutner, A., Stehlik, C., Zhang, J., Gallick, G. E. and Flynn, D. C. (2007). AFAP-110 is required for actin stress fiber formation and cell adhesion in MDA-MB-231 breast cancer cells. J Cell Physiol 213(3): 740-749.
  24. Dorfleutner, A., Bryan, N. B., Talbott, S. J., Funya, K. N., Rellick, S. L., Reed, J. C., Shi, X., Rojanasakul, Y., Flynn, D. C. and Stehlik, C. (2007). Cellular pyrin domain-only protein 2 is a candidate regulator of inflammasome activation. Infect Immun 75(3): 1484-1492.
  25. Helfer, B., Boswell, B. C., Finlay, D., Cipres, A., Vuori, K., Bong Kang, T., Wallach, D., Dorfleutner, A., Lahti, J. M., Flynn, D. C. and Frisch, S. M. (2006). Caspase-8 promotes cell motility and calpain activity under nonapoptotic conditions. Cancer Res 66(8): 4273-4278.
  26. Kroismayr, R., Baranyi, U., Stehlik, C., Dorfleutner, A., Binder, B. R. and Lipp, J. (2004). HERC5, a HECT E3 ubiquitin ligase tightly regulated in LPS activated endothelial cells. J Cell Sci 117(Pt 20): 4749-4756.
  27. Dorfleutner, A., Hintermann, E., Tarui, T., Takada, Y. and Ruf, W. (2004). Cross-talk of integrin alpha3beta1 and tissue factor in cell migration. Mol Biol Cell 15(10): 4416-4425.
  28. Stehlik, C., Kroismayr, R., Dorfleutner, A., Binder, B. R. and Lipp, J. (2004). VIGR--a novel inducible adhesion family G-protein coupled receptor in endothelial cells. FEBS Lett 569(1-3): 149-155.
  29. Belting, M., Dorrell, M. I., Sandgren, S., Aguilar, E., Ahamed, J., Dorfleutner, A., Carmeliet, P., Mueller, B. M., Friedlander, M. and Ruf, W. (2004). Regulation of angiogenesis by tissue factor cytoplasmic domain signaling. Nat Med 10(5): 502-509.
  30. Ruf, W., Dorfleutner, A. and Riewald, M. (2003). Specificity of coagulation factor signaling. J Thromb Haemost 1(7): 1495-1503.
  31. Stehlik, C., Lee, S. H., Dorfleutner, A., Stassinopoulos, A., Sagara, J. and Reed, J. C. (2003). Apoptosis-associated speck-like protein containing a caspase recruitment domain is a regulator of procaspase-1 activation. J Immunol 171(11): 6154-6163.
  32. Dorfleutner, A. and Ruf, W. (2003). Regulation of tissue factor cytoplasmic domain phosphorylation by palmitoylation. Blood 102(12): 3998-4005.
  33. Stehlik, C., Fiorentino, L., Dorfleutner, A., Bruey, J. M., Ariza, E. M., Sagara, J. and Reed, J. C. (2002). The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways. J Exp Med 196(12): 1605-1615.
2 Protocols published
In vivo Analysis of Neutrophil Infiltration during LPS-induced Peritonitis
Authors:  Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik, date: 10/05/2016, view: 4384, Q&A: 0
Bacterial lipopolysaccharide (LPS) is present in the outer membrane of Gram-negative bacteria and functions as pathogen-associated molecular pattern (PAMP) (Whitfield and Trent, 2014). LPS therefore is a potent activator of inflammatory responses ...
ASC-particle-induced Peritonitis
Authors:  Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik, date: 10/05/2016, view: 2885, Q&A: 0
In response to pathogen infection and tissue damage, inflammasome sensors such as NLRP3 and AIM2 are activated, which triggers PYRIN domain (PYD)-mediated ASC nucleation, followed by self-perpetuating ASC polymerization, which ultimately culminates ...
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