Kenji Sugiyama —BIO-PROTOCOL

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Kyosuke Nagata Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Japan
1 protocol

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Arsalan Daudi University of California
157 protocols

Nathan James MRC Laboratory of Molecular Biology
2 protocols

Sonali Chaturvedi Gladstone Institute of Virology and Immunology
3 protocols

Reviewer

Kenji Sugiyama

University of Tsukuba

Research focus

Biochemistry
Influenza virus
biochemistry

1 Author merit
Reviewer merit
Editor merit

Lab information

Nagata Lab

1 Protocol published

Purification and Identification of Novel Host-derived Factors for Influenza Virus Replication from Human Nuclear Extracts

Authors: Kenji Sugiyama and Kyosuke Nagata, date: 09/20/2016, view: 8161, Q&A: 0

Recently, we identified two host cell-derived proteins as novel stimulatory factors of influenza virus RNA replication process, termed “Influenza virus REplication Factor-2 (IREF-2)”, from human nuclear extracts (NEs) by employing biochemical ...

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3 Protocols reviewed

Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples

Authors: Konrad Herbst, Matthias Meurer, Daniel Kirrmaier, Simon Anders, Michael Knop and Viet Loan Dao Thi, date: 03/20/2021, view: 2628, Q&A: 0

During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse

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Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot

Authors: Liang Yin and Caroline S. Harwood, date: 12/05/2020, view: 911, Q&A: 0

Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we ...

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