Kenji Sugiyama —BIO-PROTOCOL

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Kyosuke Nagata Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Japan
1 protocol

Arsalan Daudi University of California
157 protocols

Stéphane Romero Ecole Polytechnique
1 protocol

Reviewer

Kenji Sugiyama

University of Tsukuba

Research focus

Biochemistry
Influenza virus
biochemistry

1 Author merit
Reviewer merit
Editor merit

Lab information

Nagata Lab

1 Protocol published

Purification and Identification of Novel Host-derived Factors for Influenza Virus Replication from Human Nuclear Extracts

Authors: Kenji Sugiyama and Kyosuke Nagata, date: 09/20/2016, view: 4443, Q&A: 0

Recently, we identified two host cell-derived proteins as novel stimulatory factors of influenza virus RNA replication process, termed “Influenza virus REplication Factor-2 (IREF-2)”, from human nuclear extracts (NEs) by employing biochemical ...

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1 Protocol reviewed

Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor

Authors: Rajan Lamichhane, Jeffrey J. Liu, Raymond F. Pauszek III and David P. Millar, date: 06/20/2017, view: 3483, Q&A: 0

Activation of G protein-coupled receptors (GPCRs) by agonist ligands is mediated by a transition from an inactive to active receptor conformation. We describe a novel single-molecule assay that monitors activation-linked conformational transitions ...

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