Aran Incharoensakdi Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand
1 protocol
Alba Blesa Universidad Francisco de Vitoria
23 protocols

Arsalan Daudi University of California
157 protocols

Chenchen Liu Kyoto University
2 protocols

Darrell Cockburn Pennsylvania State University
8 protocols

Reviewer
Simab Kanwal
  • Research scientist, Mahidol University
Research focus
  • Biochemistry
  • Molecular Biology, Metabolic engineering, Phytochemistry, Gene expression, Bacterial Toxins, Cytotoxicity
  • 1 Author merit

Education

Ph.D, Chulalongkorn, 2014

Publications

1. Kanwal, S., and Incharoensakdi, A. (2020) GABA synthesis mediated by γ-aminobutanal dehydrogenase in Synechocystis sp. PCC6803 with disrupted glutamate and α-ketoglutarate decarboxylase genes. Plant Sci. DOI: https://doi.org/10.1016/j.plantsci.2019.110287.
2. Kanwal, S., and Incharoensakdi, A. (2019) The role of GAD pathway for regulation of GABA accumulation and C/N balance in Synechocystis sp. PCC6803. J. Appl. Phycol. DOI: https://doi.org/10.1007/s10811-019-01834-5.
3. Jantaro, S., and Kanwal, S. (2017) Chapter 8 – Low-Molecular-Weight Nitrogenous Compounds (GABA and Polyamines) in Blue–Green Algae. Algal Green Chemistry-Recent Progress in Biotechnology. 1st ed. 149–169.
4. Kanwal, S., and Incharoensakdi, A. (2016) Extraction and quantification of GABA and glutamate from cyanobacterium Synechocystis sp. PCC 6803. Bio-protocol. DOI: https://dx.doi.org/10.21769/BioProtoc.1928.
5. Kanwal, S., and Incharoensakdi, A. (2016) Characterization of glutamate decarboxylase from Synechocystis sp. PCC 6803 and its role in nitrogen metabolism. Plant Physiol. Biochem. 99:59–65.
6. Kanwal, S., Khetkorn, W., and Incharoensakdi, A. (2015) GABA accumulation in response to different nitrogenous compounds in unicellular cyanobacterium Synechocystis sp. PCC 6803. Curr. Microbiol. 70:96–102
7. Kanwal, S., Rastogi, R,P., and Incharoensakdi, A. (2014) Glutamate decarboxylase activity and gamma-aminobutyric acid content in Synechocystis sp. PCC 6803 under osmotic stress and different carbon sources. J. Appl. Phycol. 26:2327–2333
8. Haq, I.U., Ullah, N., Bibi, G., Kanwal, S., Ahmad, M.S., and Mirza, B. (2012) Antioxidant and cytotoxic activities and phytochemical analysis of Euphorbia wallichii root extract and its fractions. I.J.P.R. 11: 241-249.
9. Kanwal, S., Ullah, N., Haq, I.U., Afzal, I., and Mirza, B. (2011) Antioxidant, antitumor activities and phytochemical investigation of Hedera nepalensis k.koch, an important medicinal plant from Pakistan. Pak. J. Bot. 43: 85-89.
1 Protocol published
Extraction and Quantification of GABA and Glutamate from Cyanobacterium Synechocystis sp. PCC 6803
Authors:  Simab Kanwal and Aran Incharoensakdi, date: 09/20/2016, view: 9260, Q&A: 0
GABA (γ-amino butyric acid) is a biologically active four carbon non-protein amino acid found in prokaryotes and eukaryotes. Glutamate is a five carbon α-amino acid which can be converted to GABA catalyzed by the enzyme glutamate decarboxylase. In ...
4 Protocols reviewed
Rapid Isolation and Purification of Secreted Bacteriocins from Streptococcus mutans and Other Lactic Acid Bacteria
Authors:  Marcus Cheng, Siew-Ging Gong and Céline M. Lévesque, date: 11/20/2020, view: 1055, Q&A: 0
Bacteriocins are small ribosomally synthesized antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for low pH. Bacteriocins kill ...
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Construction and Validation of A Low-cost, Small-scale, Multiplex Continuous Culturing System for Microorganisms
Authors:  Lilit Tonoyan, Freddy Guihéneuf, Ruari Friel and Vincent O’Flaherty, date: 11/05/2020, view: 687, Q&A: 0

This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (i.e., bacteria, yeast, microalgae). The continuous culture device can

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