Chia-Yu Wang Food Science and Human Nutrition Department, University of Florida, USA
1 protocol

Supak Jenkitkasemwong Food Science and Human Nutrition Department, University of Florida, USA
1 protocol

Mitchell D. Knutson
  • Food Science and Human Nutrition Department, University of Florida, USA
  • 1 Author merit


Ph.D in Nutrition, Department of Nutrition, University of California at Berkeley, 1998

Current position

Professor of Nutritional Biochemistry, Food Science and Human Nutrition Department, University of Florida, Gainesville, FL, USA

Publications (since 2005)

  1. Jenkitkasemwong, S., Wang, C. Y., Coffey, R., Zhang, W., Chan, A., Biel, T., Kim, J. S., Hojyo, S., Fukada, T. and Knutson, M. D. (2015). SLC39A14 Is Required for the Development of Hepatocellular Iron Overload in Murine Models of Hereditary Hemochromatosis. Cell Metab 22(1): 138-150.

  2. Tripathi, A. K., Haldar, S., Qian, J., Beserra, A., Suda, S., Singh, A., Hopfer, U., Chen, S. G., Garrick, M. D., Turner, J. R., Knutson, M. D. and Singh, N. (2015). Prion protein functions as a ferrireductase partner for ZIP14 and DMT1. Free Radic Biol Med 84: 322-330.

  3. Zhao, N., Zhang, A. S., Worthen, C., Knutson, M. D. and Enns, C. A. (2014). An iron-regulated and glycosylation-dependent proteasomal degradation pathway for the plasma membrane metal transporter ZIP14. Proc Natl Acad Sci U S A 111(25): 9175-9180.

  4. Coffey, R., Nam, H. and Knutson, M. D. (2014). Microarray analysis of rat pancreas reveals altered expression of Alox15 and regenerating islet-derived genes in response to iron deficiency and overload. PLoS One 9(1): e86019.

  5. Wang, C. Y. and Knutson, M. D. (2013). Hepatocyte divalent metal-ion transporter-1 is dispensable for hepatic iron accumulation and non-transferrin-bound iron uptake in mice. Hepatology 58(2): 788-798.

  6. Nam, H., Wang, C. Y., Zhang, L., Zhang, W., Hojyo, S., Fukada, T. and Knutson, M. D. (2013). ZIP14 and DMT1 in the liver, pancreas, and heart are differentially regulated by iron deficiency and overload: implications for tissue iron uptake in iron-related disorders. Haematologica 98(7): 1049-1057.

  7. Guo, X., Zhang, Z., Zhang, F., Tao, Y., An, P., Wu, Q., Wang, C. Y., Knutson, M. D. and Wang, F. (2013). Fine-mapping and genetic analysis of the loci affecting hepatic iron overload in mice. PLoS One 8(5): e63280.

  8. Wang, C. Y., Jenkitkasemwong, S., Duarte, S., Sparkman, B. K., Shawki, A., Mackenzie, B. and Knutson, M. D. (2012). ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. J Biol Chem 287(41): 34032-34043.

  9. Jenkitkasemwong, S., Wang, C. Y., Mackenzie, B. and Knutson, M. D. (2012). Physiologic implications of metal-ion transport by ZIP14 and ZIP8. Biometals 25(4): 643-655.

  10. Nam, H. and Knutson, M. D. (2012). Effect of dietary iron deficiency and overload on the expression of ZIP metal-ion transporters in rat liver. Biometals 25(1): 115-124.

  11. Xu, J., Jia, Z., Knutson, M. D. and Leeuwenburgh, C. (2012). Impaired iron status in aging research. Int J Mol Sci 13(2): 2368-2386.

  12. Xu, J., Hwang, J. C., Lees, H. A., Wohlgemuth, S. E., Knutson, M. D., Judge, A. R., Dupont-Versteegden, E. E., Marzetti, E. and Leeuwenburgh, C. (2012). Long-term perturbation of muscle iron homeostasis following hindlimb suspension in old rats is associated with high levels of oxidative stress and impaired recovery from atrophy. Exp Gerontol 47(1): 100-108.

  13. Pinilla-Tenas, J. J., Sparkman, B. K., Shawki, A., Illing, A. C., Mitchell, C. J., Zhao, N., Liuzzi, J. P., Cousins, R. J., Knutson, M. D. and Mackenzie, B. (2011). Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. Am J Physiol Cell Physiol 301(4): C862-871.

  14. Zhang, Z., Zhang, F., An, P., Guo, X., Shen, Y., Tao, Y., Wu, Q., Zhang, Y., Yu, Y., Ning, B., Nie, G., Knutson, M. D., Anderson, G. J. and Wang, F. (2011). Ferroportin1 deficiency in mouse macrophages impairs iron homeostasis and inflammatory responses. Blood 118(7): 1912-1922.

  15. Zhao, N., Gao, J., Enns, C. A. and Knutson, M. D. (2010). ZRT/IRT-like protein 14 (ZIP14) promotes the cellular assimilation of iron from transferrin. J Biol Chem 285(42): 32141-32150.

  16. Knutson, M. D. (2010). Iron-sensing proteins that regulate hepcidin and enteric iron absorption. Annu Rev Nutr 30: 149-171.

  17. Jenkitkasemwong, S., Broderius, M., Nam, H., Prohaska, J. R. and Knutson, M. D. (2010). Anemic copper-deficient rats, but not mice, display low hepcidin expression and high ferroportin levels. J Nutr 140(4): 723-730.

  18. Collins, J. F., Prohaska, J. R. and Knutson, M. D. (2010). Metabolic crossroads of iron and copper. Nutr Rev 68(3): 133-147.

  19. Hansen, S. L., Ashwell, M. S., Moeser, A. J., Fry, R. S., Knutson, M. D. and Spears, J. W. (2010). High dietary iron reduces transporters involved in iron and manganese metabolism and increases intestinal permeability in calves. J Dairy Sci 93(2): 656-665.

  20. Koeppen, A. H., Morral, J. A., Davis, A. N., Qian, J., Petrocine, S. V., Knutson, M. D., Gibson, W. M., Cusack, M. J. and Li, D. (2009). The dorsal root ganglion in Friedreich's ataxia. Acta Neuropathol 118(6): 763-776.

  21. Hepburn, J. J., Arthington, J. D., Hansen, S. L., Spears, J. W. and Knutson, M. D. (2009). Technical note: copper chaperone for copper, zinc superoxide dismutase: a potential biomarker for copper status in cattle. J Anim Sci 87(12): 4161-4166.

  22. Genter, M. B., Kendig, E. L. and Knutson, M. D. (2009). Uptake of materials from the nasal cavity into the blood and brain: are we finally beginning to understand these processes at the molecular level? Ann N Y Acad Sci 1170: 623-628.

  23. Knutson, M. D. (2009). Into the matrix: regulation of the iron regulatory hormone hepcidin by matriptase-2. Nutr Rev 67(5): 284-288.

  24. Andriopoulos, B., Jr., Corradini, E., Xia, Y., Faasse, S. A., Chen, S., Grgurevic, L., Knutson, M. D., Pietrangelo, A., Vukicevic, S., Lin, H. Y. and Babitt, J. L. (2009). BMP6 is a key endogenous regulator of hepcidin expression and iron metabolism. Nat Genet 41(4): 482-487.

  25. Aydemir, F., Jenkitkasemwong, S., Gulec, S. and Knutson, M. D. (2009). Iron loading increases ferroportin heterogeneous nuclear RNA and mRNA levels in murine J774 macrophages. J Nutr 139(3): 434-438.

  26. Collins, J. F., Wessling-Resnick, M. and Knutson, M. D. (2008). Hepcidin regulation of iron transport. J Nutr 138(11): 2284-2288.

  27. Seo, A. Y., Xu, J., Servais, S., Hofer, T., Marzetti, E., Wohlgemuth, S. E., Knutson, M. D., Chung, H. Y. and Leeuwenburgh, C. (2008). Mitochondrial iron accumulation with age and functional consequences. Aging Cell 7(5): 706-716.

  28. Knutson, M. D. and Leeuwenburgh, C. (2008). Resveratrol and novel potent activators of SIRT1: effects on aging and age-related diseases. Nutr Rev 66(10): 591-596.

  29. Xu, J., Knutson, M. D., Carter, C. S. and Leeuwenburgh, C. (2008). Iron accumulation with age, oxidative stress and functional decline. PLoS One 3(8): e2865.

  30. Gao, J., Zhao, N., Knutson, M. D. and Enns, C. A. (2008). The hereditary hemochromatosis protein, HFE, inhibits iron uptake via down-regulation of Zip14 in HepG2 cells. J Biol Chem 283(31): 21462-21468.

  31. King, S. M., Donangelo, C. M., Knutson, M. D., Walter, P. B., Ames, B. N., Viteri, F. E. and King, J. C. (2008). Daily supplementation with iron increases lipid peroxidation in young women with low iron stores. Exp Biol Med (Maywood) 233(6): 701-707.

  32. Hofer, T., Marzetti, E., Xu, J., Seo, A. Y., Gulec, S., Knutson, M. D., Leeuwenburgh, C. and Dupont-Versteegden, E. E. (2008). Increased iron content and RNA oxidative damage in skeletal muscle with aging and disuse atrophy. Exp Gerontol 43(6): 563-570.

  33. Koeppen, A. H., Michael, S. C., Knutson, M. D., Haile, D. J., Qian, J., Levi, S., Santambrogio, P., Garrick, M. D. and Lamarche, J. B. (2007). The dentate nucleus in Friedreich's ataxia: the role of iron-responsive proteins. Acta Neuropathol 114(2): 163-173.

  34. Knutson, M. D. (2007). Steap proteins: implications for iron and copper metabolism. Nutr Rev 65(7): 335-340.

  35. Imrich, A., Ning, Y., Lawrence, J., Coull, B., Gitin, E., Knutson, M. and Kobzik, L. (2007). Alveolar macrophage cytokine response to air pollution particles: oxidant mechanisms. Toxicol Appl Pharmacol 218(3): 256-264.

  36. Liuzzi, J. P., Aydemir, F., Nam, H., Knutson, M. D. and Cousins, R. J. (2006). Zip14 (Slc39a14) mediates non-transferrin-bound iron uptake into cells. Proc Natl Acad Sci U S A 103(37): 13612-13617.

  37. Chlosta, S., Fishman, D. S., Harrington, L., Johnson, E. E., Knutson, M. D., Wessling-Resnick, M. and Cherayil, B. J. (2006). The iron efflux protein ferroportin regulates the intracellular growth of Salmonella enterica. Infect Immun 74(5): 3065-3067.

  38. Brain, J. D., Heilig, E., Donaghey, T. C., Knutson, M. D., Wessling-Resnick, M. and Molina, R. M. (2006). Effects of iron status on transpulmonary transport and tissue distribution of Mn and Fe. Am J Respir Cell Mol Biol 34(3): 330-337.

  39. Michael, S., Petrocine, S. V., Qian, J., Lamarche, J. B., Knutson, M. D., Garrick, M. D. and Koeppen, A. H. (2006). Iron and iron-responsive proteins in the cardiomyopathy of Friedreich's ataxia. Cerebellum 5(4): 257-267.

  40. Liuzzi, J. P., Lichten, L. A., Rivera, S., Blanchard, R. K., Aydemir, T. B., Knutson, M. D., Ganz, T. and Cousins, R. J. (2005). Interleukin-6 regulates the zinc transporter Zip14 in liver and contributes to the hypozincemia of the acute-phase response. Proc Natl Acad Sci U S A 102(19): 6843-6848.

  41. Knutson, M. D., Oukka, M., Koss, L. M., Aydemir, F. and Wessling-Resnick, M. (2005). Iron release from macrophages after erythrophagocytosis is up-regulated by ferroportin 1 overexpression and down-regulated by hepcidin. Proc Natl Acad Sci U S A 102(5): 1324-1328.

1 Protocol published
Measurement of Transferrin- and Non-transferrin-bound Iron Uptake by Mouse Tissues
Authors:  Supak Jenkitkasemwong, Chia-Yu Wang and Mitchell D. Knutson, date: 09/05/2016, view: 2381, Q&A: 0
Iron in blood plasma is bound to its transport protein transferrin, which delivers iron to most tissues. In iron overload and certain pathological conditions, the carrying capacity of transferrin can become exceeded, giving rise to ...