Janna Bigalke Department of Molecular Biology and Microbiology, Tufts University School of Medicine, USA
1 protocol

Ekaterina E. Heldwein
  • Department of Molecular Biology and Microbiology, Tufts University School of Medicine, USA
  • 1 Author merit


Ph.D., Biochemistry, Oregon Health and Sciences University, 1999

Current position

Associate Professor with tenure, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA

Publications (since 2010)

  1. Metrick, C. M. and Heldwein, E. E. (2016). Novel Structure and Unexpected RNA-Binding Ability of the C-Terminal Domain of Herpes Simplex Virus 1 Tegument Protein UL21. J Virol 90(12): 5759-5769.
  2. Meyer, P. A., Socias, S., Key, J., Ransey, E., Tjon, E. C., Buschiazzo, A., Lei, M., Botka, C., Withrow, J., Neau, D., Rajashankar, K., Anderson, K. S., Baxter, R. H., Blacklow, S. C., Boggon, T. J., Bonvin, A. M., Borek, D., Brett, T. J., Caflisch, A., Chang, C. I., Chazin, W. J., Corbett, K. D., Cosgrove, M. S., Crosson, S., Dhe-Paganon, S., Di Cera, E., Drennan, C. L., Eck, M. J., Eichman, B. F., Fan, Q. R., Ferre-D'Amare, A. R., Christopher Fromme, J., Garcia, K. C., Gaudet, R., Gong, P., Harrison, S. C., Heldwein, E. E., Jia, Z., Keenan, R. J., Kruse, A. C., Kvansakul, M., McLellan, J. S., Modis, Y., Nam, Y., Otwinowski, Z., Pai, E. F., Pereira, P. J., Petosa, C., Raman, C. S., Rapoport, T. A., Roll-Mecak, A., Rosen, M. K., Rudenko, G., Schlessinger, J., Schwartz, T. U., Shamoo, Y., Sondermann, H., Tao, Y. J., Tolia, N. H., Tsodikov, O. V., Westover, K. D., Wu, H., Foster, I., Fraser, J. S., Maia, F. R., Gonen, T., Kirchhausen, T., Diederichs, K., Crosas, M. and Sliz, P. (2016). Data publication with the structural biology data grid supports live analysis. Nat Commun 7: 10882.
  3. Bigalke, J. M. and Heldwein, E. E. (2015). Structural basis of membrane budding by the nuclear egress complex of herpesviruses. EMBO J 34(23): 2921-2936.
  4. Burke, H. G. and Heldwein, E. E. (2015). Crystal Structure of the Human Cytomegalovirus Glycoprotein B. PLoS Pathog 11(10): e1005227.
  5. Rogalin, H. B. and Heldwein, E. E. (2015). Interplay between the Herpes Simplex Virus 1 gB Cytodomain and the gH Cytotail during Cell-Cell Fusion. J Virol 89(24): 12262-12272.
  6. Metrick, C. M., Chadha, P. and Heldwein, E. E. (2015). The unusual fold of herpes simplex virus 1 UL21, a multifunctional tegument protein. J Virol 89(5): 2979-2984.
  7. Bigalke, J. M., Heuser, T., Nicastro, D. and Heldwein, E. E. (2014). Membrane deformation and scission by the HSV-1 nuclear egress complex. Nat Commun 5: 4131.
  8. Pitts, J. D., Klabis, J., Richards, A. L., Smith, G. A. and Heldwein, E. E. (2014). Crystal structure of the herpesvirus inner tegument protein UL37 supports its essential role in control of viral trafficking. J Virol 88(10): 5462-5473.
  9. Silverman, J. L. and Heldwein, E. E. (2013). Mutations in the cytoplasmic tail of herpes simplex virus 1 gH reduce the fusogenicity of gB in transfected cells. J Virol 87(18): 10139-10147.
  10. Vitu, E., Sharma, S., Stampfer, S. D. and Heldwein, E. E. (2013). Extensive mutagenesis of the HSV-1 gB ectodomain reveals remarkable stability of its postfusion form. J Mol Biol 425(11): 2056-2071.
  11. Sharma, S., Wisner, T. W., Johnson, D. C. and Heldwein, E. E. (2013). HCMV gB shares structural and functional properties with gB proteins from other herpesviruses. Virology 435(2): 239-249.
  12. Silverman, J. L., Greene, N. G., King, D. S. and Heldwein, E. E. (2012). Membrane requirement for folding of the herpes simplex virus 1 gB cytodomain suggests a unique mechanism of fusion regulation. J Virol 86(15): 8171-8184.
  13. Cairns, T. M., Whitbeck, J. C., Lou, H., Heldwein, E. E., Chowdary, T. K., Eisenberg, R. J. and Cohen, G. H. (2011). Capturing the herpes simplex virus core fusion complex (gB-gH/gL) in an acidic environment. J Virol 85(13): 6175-6184.
  14. Stampfer, S. D., Lou, H., Cohen, G. H., Eisenberg, R. J. and Heldwein, E. E. (2010). Structural basis of local, pH-dependent conformational changes in glycoprotein B from herpes simplex virus type 1. J Virol 84(24): 12924-12933.
  15. Chowdary, T. K., Cairns, T. M., Atanasiu, D., Cohen, G. H., Eisenberg, R. J. and Heldwein, E. E. (2010). Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL. Nat Struct Mol Biol 17(7): 882-888.
  16. Chowdary, T. K. and Heldwein, E. E. (2010). Syncytial phenotype of C-terminally truncated herpes simplex virus type 1 gB is associated with diminished membrane interactions. J Virol 84(10): 4923-4935.
  17. Silverman, J. L., Sharma, S., Cairns, T. M. and Heldwein, E. E. (2010). Fusion-deficient insertion mutants of herpes simplex virus type 1 glycoprotein B adopt the trimeric postfusion conformation. J Virol 84(4): 2001-2012.
  18. Hannah, B. P., Heldwein, E. E., Bender, F. C., Cohen, G. H. and Eisenberg, R. J. (2007). Mutational evidence of internal fusion loops in herpes simplex virus glycoprotein B. J Virol 81(9): 4858-4865.
  19. Bender, F. C., Samanta, M., Heldwein, E. E., de Leon, M. P., Bilman, E., Lou, H., Whitbeck, J. C., Eisenberg, R. J. and Cohen, G. H. (2007). Antigenic and mutational analyses of herpes simplex virus glycoprotein B reveal four functional regions. J Virol 81(8): 3827-3841.
  20. Heldwein, E. E., Lou, H., Bender, F. C., Cohen, G. H., Eisenberg, R. J. and Harrison, S. C. (2006). Crystal structure of glycoprotein B from herpes simplex virus 1. Science 313(5784): 217-220.
  21. Heldwein, E. E., Macia, E., Wang, J., Yin, H. L., Kirchhausen, T. and Harrison, S. C. (2004). Crystal structure of the clathrin adaptor protein 1 core. Proc Natl Acad Sci U S A 101(39): 14108-14113.
  22. Godsey, M. H., Zheleznova Heldwein, E. E. and Brennan, R. G. (2002). Structural biology of bacterial multidrug resistance gene regulators. J Biol Chem 277(43): 40169-40172.
  23. Heldwein, E. E. and Brennan, R. G. (2001). Crystal structure of the transcription activator BmrR bound to DNA and a drug. Nature 409(6818): 378-382.
  24. Zheleznova, E. E., Crosa, J. H. and Brennan, R. G. (2000). Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion. J Bacteriol 182(21): 6264-6267.
  25. Vazquez-Laslop, N., Zheleznova, E. E., Markham, P. N., Brennan, R. G. and Neyfakh, A. A. (2000). Recognition of multiple drugs by a single protein: a trivial solution of an old paradox. Biochem Soc Trans 28(4): 517-520.
  26. Jardim, A., Liu, W., Zheleznova, E. and Ullman, B. (2000). Peroxisomal targeting signal-1 receptor protein PEX5 from Leishmania donovani. Molecular, biochemical, and immunocytochemical characterization. J Biol Chem 275(18): 13637-13644.
  27. Zheleznova, E. E., Markham, P., Edgar, R., Bibi, E., Neyfakh, A. A. and Brennan, R. G. (2000). A structure-based mechanism for drug binding by multidrug transporters. Trends Biochem Sci 25(2): 39-43.
1 Protocol published
Expression, Purification and Crystallization of the Herpesvirus Nuclear Egress Complex (NEC)
Authors:  Janna M. Bigalke and Ekaterina E. Heldwein, date: 07/20/2016, view: 4409, Q&A: 0
The protocol describes the production and crystallization of the soluble form of the nuclear egress complex (NEC) from Herpes simplex virus 1 and Pseudorabies virus. The NEC is a heterodimer that consists of conserved proteins UL31 and UL34. NEC ...
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