Gunther Doehlemann Botanical Institute and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Germany, Germany,
3 protocols

Elaine Jaeger Botanical Institute and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Germany, Germany,
1 protocol
Adam Idoine Carnegie Institution
12 protocols

Anna Ochocka-Fox University of Edinburgh
1 protocol

Cynthia Monaco University of Rochester Medical Center
1 protocol

Dheeraj Rathore Teagasc
1 protocol

Editor
Amey Redkar
  • Post-Doc, Department of Genetics, University of Cordoba
Research focus
  • Plant science
  • Plant-Microbe Interactions, Effectors, Microbiology
  • 3 Author merit

Education

PhD

Lab information

Di Pietro 's lab

Publications

1. Redkar A*, Matei A, Doehlemann G. (2017) Insights into host cell modulation and induction of new cells in the corn smut Ustilago maydis. Frontiers in Plant Science (in press)
2. Redkar A and Doehlemann G. (2016). Ustilago maydis virulence assays in maize. Bio-protocol 6(6): e1760. http://www.bio-protocol.org/e1760
3. Redkar A* and Doehlemann, G. (2016) EdU based DNA synthesis and cell proliferation assay in maize infected by the smut fungus Ustilago maydis. Bio-protocol 6(6): e1761. http://www.bio-protocol.org/e1761
4. Redkar A, Villajuana-Bonequi M, Doehlemann G. (2015). Conservation of the Ustilago maydis effector See1 in related smuts. Plant Signalling and Behaviour DOI:10.1080/15592324.2015.1086855
5. Redkar A, Hoser R, Schilling L, Zechmann B, Krzymowska M, Walbot V, Doehlemann G. (2015). A secreted effector protein of Ustilago maydis guides maize leaf cells to form tumors. The Plant Cell 27 (4), pp: 1332-51.
6. Schilling L#, Matei A#, Redkar A, Walbot V, Doehlemann G. (2014). Virulence of the maize smut Ustilago maydis is shaped by organ-specific effectors. Molecular Plant Pathology 15(8), pp: 780-89.
7. van der Linde K, Mueller A N, Redkar A, Schilling L, Doehlemann G. (2013). Brandpilz mit pep: Ustilago maydis und das pflanzliche Immunsystem . Biospektrum 13(4), pp: 140-42.
8. Kuvalekar A, Redkar A, Gandhe K, Harsulkar A. (2011). Peroxidase and polyphenol oxidase activities in compatible host-pathogen interaction in Jasminum officinale and Uromyces hobsonii: Insights into the susceptibility of host. New Zealand Journal of Botany 49(3), pp: 351-59.
3 Protocols published
Visualization of Growth and Morphology of Fungal Hyphae in planta Using WGA-AF488 and Propidium Iodide Co-staining
Authors:  Amey Redkar, Elaine Jaeger and Gunther Doehlemann, date: 07/20/2018, view: 938, Q&A: 0
Fungal pathogens colonizing plants show a varying degree of symptoms. Microscopy techniques have been used to study the infection and proliferation of fungal hyphae inside the host. One of the best optimized and commonly used method is the ...
EdU Based DNA Synthesis and Cell Proliferation Assay in Maize Infected by the Smut Fungus Ustilago maydis
Authors:  Amey Redkar and Gunther Doehlemann, date: 03/20/2016, view: 3952, Q&A: 0
The basidiomycetous smut fungus Ustilago maydis (U. maydis) infects all aerial parts of its host plant maize (Zea mays L.). Infection is seen in the form of prominent tumorous symptoms after the establishment of a ...
4 Protocols reviewed
A Method for Extracting the Nuclear Scaffold from the Chromatin Network
Authors:  Junjie Chen, Boon Heng Dennis Teo and Jinhua Lu, date: 04/20/2018, view: 1418, Q&A: 0
Each cell contains many large DNA polymers packed in a nucleus of approx. 10 μm in diameter. With histones, these DNA polymers are known to form chromatins. How chromatins further compact in the nucleus is unclear but it inevitably depends on an ...
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Chromatin Affinity Purification (ChAP) from Arabidopsis thaliana Rosette Leaves Using in vivo Biotinylation System
Authors:  Weronika Sura and Piotr A. Ziolkowski, date: 01/05/2018, view: 1750, Q&A: 0
Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which in vivo ...
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8 Protocols edited
Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
Authors:  Yan Bao and Diane C. Bassham, date: 12/05/2018, view: 379, Q&A: 0
Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein ...
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Electron Microscopic Detection of Proteins and Protein Complexes in Mammalian Cells Using APEX-tagged, Conditionally Stable Nanobodies
Authors:  Thomas E. Hall, James Rae, Nicholas Ariotti and Robert G. Parton, date: 11/20/2018, view: 438, Q&A: 0
The recent development of 3D electron microscopic techniques for cells and tissues has necessitated the development of new methods for the detection of proteins and protein-complexes in situ. The development of new genetic tags, such as the ...
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