Steve Jean Université de Sherbrooke
2 protocols

Amy A. Kiger
  • Division of Biological Sciences, University of California, USA
Research focus
  • Biochemistry
  • 2 Author merit


Ph.D. in Developmental Biology, Stanford University School of Medicine, Stanford, CA, 2000

Current position

Associate Professor, Section of Cell & Developmental Biology, University of California, San Diego, La Jolla, CA


  1. Jean, S., Cox, S., Nassari, S. and Kiger, A. A. (2015). Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion. EMBO Rep 16(3): 297-311.
  2. Jean, S. and Kiger, A. A. (2014). Classes of phosphoinositide 3-kinases at a glance. J Cell Sci 127(Pt 5): 923-928.
  3. Jean, S. and Kiger, A. A. (2012). Coordination between RAB GTPase and phosphoinositide regulation and functions. Nat Rev Mol Cell Biol 13(7): 463-470.
  4. Jean, S., S. Cox, E.J. Schmidt, F.L. Robinson and Kiger,A.A. 2012. Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling. Mol Biol Cell 23(14):2723-40.
  5. Rohn, J. L., Sims, D., Liu, T., Fedorova, M., Schock, F., Dopie, J., Vartiainen, M. K., Kiger, A. A., Perrimon, N. and Baum, B. (2011). Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype. J Cell Biol 194(5): 789-805.
  6. Jean, S. and Kiger, A. A. (2012). Coordination between RAB GTPase and phosphoinositide regulation and functions. Nat Rev Mol Cell Biol 13(7): 463-470.
  7. Kadandale, P. and Kiger, A. A. (2010). Role of selective autophagy in cellular remodeling: "self-eating" into shape. Autophagy 6(8): 1194-1195.
  8. Velichkova, M., Juan, J., Kadandale, P., Jean, S., Ribeiro, I., Raman, V., Stefan, C. and Kiger, A. A. (2010). Drosophila Mtm and class II PI3K coregulate a PI(3)P pool with cortical and endolysosomal functions. J Cell Biol 190(3): 407-425.
  9. Kadandale, P., Stender, J. D., Glass, C. K. and Kiger, A. A. (2010). Conserved role for autophagy in Rho1-mediated cortical remodeling and blood cell recruitment. Proc Natl Acad Sci U S A 107(23): 10502-10507.
  10. Asano Y, Jiménez-Dalmaroni A, Liverpool TB, Marchetti MC, Giomi L, Kiger A, Duke T, Baum B. 2009. Pak3 inhibits local actin filament formation to regulate global cell polarity. HFSP J. 3(3): 194-203.
  11. Flockhart I, Booker M, Kiger A, Boutros M, Armknecht S, Ramadan N, Richardson K, Xu A, Perrimon N, Mathey-Prevot B. 2006. FlyRNAi: the Drosophila RNAi screening center database. Nucleic Acids Res. 34: D489-94.
  12. Armknecht, S., M. Boutros, A. Kiger, K. Nybakken, B. Mathey-Prevot and N. Perrimon. 2005. High-throughput RNAi screens in Drosophila tissue culture cells. Methods Enzymol. 392: 55-73.
  13. Eggert, U. S., Kiger, A. A., Richter, C., Perlman, Z. E., Perrimon, N., Mitchison, T. J. and Field, C. M. (2004). Parallel chemical genetic and genome-wide RNAi screens identify cytokinesis inhibitors and targets. PLoS Biol 2(12): e379.
  14. Schlesinger, A., A. Kiger, N. Perrimon and B.-Z. Shilo. 2004. Small wing PLC-gamma is required for ER retention of cleaved Spitz during eye development in Drosophila. Dev. Cell, 7(4): 535-545.
  15. Schulz, C., Kiger, A. A., Tazuke, S. I., Yamashita, Y. M., Pantalena-Filho, L. C., Jones, D. L., Wood, C. G. and Fuller, M. T. (2004). A misexpression screen reveals effects of bag-of-marbles and TGF beta class signaling on the Drosophila male germ-line stem cell lineage. Genetics 167(2): 707-723.
  16. Boutros, M., Kiger, A. A., Armknecht, S., Kerr, K., Hild, M., Koch, B., Haas, S. A., Paro, R., Perrimon, N. and Heidelberg Fly Array, C. (2004). Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 303(5659): 832-835.
  17. Kiger, A. A., Baum, B., Jones, S., Jones, M. R., Coulson, A., Echeverri, C. and Perrimon, N. (2003). A functional genomic analysis of cell morphology using RNA interference. J Biol 2(4): 27.
  18. Bashirullah, A., Pasquinelli, A. E., Kiger, A. A., Perrimon, N., Ruvkun, G. and Thummel, C. S. (2003). Coordinate regulation of small temporal RNAs at the onset of Drosophila metamorphosis. Dev Biol 259(1): 1-8.
  19. Kiger, A. A., Jones, D. L., Schulz, C., Rogers, M. B. and Fuller, M. T. (2001). Stem cell self-renewal specified by JAK-STAT activation in response to a support cell cue. Science 294(5551): 2542-2545.
  20. Kiger, A. A., White-Cooper, H. and Fuller, M. T. (2000). Somatic support cells restrict germline stem cell self-renewal and promote differentiation. Nature 407(6805): 750-754.
  21. Kiger, A. A., Gigliotti, S. and Fuller, M. T. (1999). Developmental genetics of the essential Drosophila nucleoporin nup154: allelic differences due to an outward-directed promoter in the P-element 3' end. Genetics 153(2): 799-812.
2 Protocols published
VAMP8-3xHA Uptake Assay in HeLa Cells
Authors:  Steve Jean and Amy A. Kiger, date: 02/20/2016, view: 6720, Q&A: 0
Transmembrane proteins are rarely exclusively localized to a specific vesicle or an organelle. Most transmembrane proteins undergo complicated trafficking routes. Thus, transmembrane proteins are under constant flux, and at steady state, found on a ...
RAB21 Activity Assay Using GST-fused APPL1
Authors:  Steve Jean and Amy A. Kiger, date: 02/20/2016, view: 6688, Q&A: 0
The Rab family of small GTPases are essential regulators of membrane trafficking events. As with other small GTPase families, Rab GTPases cycle between an inactive GDP- bound state and an active GTP-bound state. Guanine nucleotide exchange factors ...
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