Petros Papadopoulos Hematology, Hospital Clinico San Carlos , Madrid
1 protocol

Vishal Salunkhe Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre (AMC), University of Amsterdam (UvA), The Netherlands
1 protocol

Laura Gutiérrez
  • Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre (AMC), Amsterdam
Research focus
  • Cell biology
  • 1 Author merit


Ph.D. in Cell Biology, Department of Cell Biology, ErasmusMC, University of Rotterdam, The Netherlands, 2005

Current position

Ramón y Cajal Research Fellow, Principal Investigator, Dept. of Hematology, Hospital Clínico San Carlos, Instituto de Investigación Sanitaria San Carlos (IdISSC), Madrid, Spain

Publications (since 2005)

  1. Scheenstra, M. R., Salunkhe, V., De Cuyper, I. M., Hoogenboezem, M., Li, E., Kuijpers, T. W., van den Berg, T. K. and Gutiérrez, L. (2015). Characterization of hematopoietic GATA transcription factor expression in mouse and human dendritic cells. Blood Cells Mol Dis 55(4): 293-303.
  2. Papadopoulos, P., Gutiérrez, L., Demmers, J., Scheer, E., Pourfarzad, F., Papageorgiou, D. N., Karkoulia, E., Strouboulis, J., van de Werken, H. J., van der Linden, R., Vandenberghe, P., Dekkers, D. H., Philipsen, S., Grosveld, F. and Tora, L. (2015). TAF10 Interacts with the GATA1 Transcription Factor and Controls Mouse Erythropoiesis. Mol Cell Biol 35(12): 2103-2118.
  3. Meinders, M., Kulu, D. I., van de Werken, H. J., Hoogenboezem, M., Janssen, H., Brouwer, R. W., van Ijcken, W. F., Rijkers, E. J., Demmers, J. A., Kruger, I., van den Berg, T. K., Suske, G., Gutiérrez, L.* and Philipsen, S.* (2015). Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation and platelet formation and function. Blood 125(12): 1957-1967.
  4. Salunkhe, V., van der Meer, P. F., de Korte, D., Seghatchian, J.* and Gutiérrez, L.* (2015). Development of blood transfusion product pathogen reduction treatments: a review of methods, current applications and demands. Transfus Apher Sci 52(1): 19-34.
  5. Zeddies, S., De Cuyper, I. M., van der Meer, P. F., Daal, B. B., de Korte, D., Gutiérrez, L.* and Thijssen-Timmer, D. C.* (2014). Pathogen reduction treatment using riboflavin and ultraviolet light impairs platelet reactivity toward specific agonists in vitro. Transfusion 54(9): 2292-2300.
  6. Bergink, S., Theil, A. F., Toussaint, W., De Cuyper, I. M., Kulu, D. I., Clapes, T., van der Linden, R., Demmers, J. A., Mul, E. P., van Alphen, F. P., Marteijn, J. A., van Gent, T., Maas, A., Robin, C., Philipsen, S., Vermeulen, W., Mitchell, J. R.* and Gutiérrez, L.* (2013). Erythropoietic defect associated with reduced cell proliferation in mice lacking the 26S proteasome shuttling factor Rad23b. Mol Cell Biol 33(19): 3879-3892.
  7. Fujita, R., Takayama-Tsujimoto, M., Satoh, H., Gutiérrez, L., Aburatani, H., Fujii, S., Sarai, A., Bresnick, E. H., Yamamoto, M. and Motohashi, H. (2013). NF-E2 p45 is important for establishing normal function of platelets. Mol Cell Biol 33(14): 2659-2670.
  8. De Cuyper, I. M., Meinders, M., van de Vijver, E., de Korte, D., Porcelijn, L., de Haas, M., Eble, J. A., Seeger, K., Rutella, S., Pagliara, D., Kuijpers, T. W., Verhoeven, A. J., van den Berg, T. K. and Gutiérrez, L. (2013). A novel flow cytometry-based platelet aggregation assay. Blood 121(10): e70-80.
  9. Papadopoulos, P., Gutiérrez, L., van der Linden, R., Kong, A. S. J., Maas, A., Drabek, D., Patrinos, G. P., Philipsen, S. and Grosveld, F. (2012). A dual reporter mouse model of the human beta-globin locus: applications and limitations. PLoS One 7(12): e51272.
  10. Drabek, K.*, Gutiérrez, L.*, Vermeij, M., Clapes, T., Patel, S. R., Boisset, J. C., van Haren, J., Pereira, A. L., Liu, Z., Akinci, U., Nikolic, T., van Ijcken, W., van den Hout, M., Meinders, M., Melo, C., Sambade, C., Drabek, D., Hendriks, R. W., Philipsen, S., Mommaas, M., Grosveld, F., Maiato, H., Italiano, J. E., Jr., Robin, C. and Galjart, N. (2012). The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem cell maintenance. Cell Rep 2(4): 781-788.
  11. van de Vijver, E., De Cuyper, I. M., Gerrits, A. J., Verhoeven, A. J., Seeger, K., Gutiérrez, L., van den Berg, T. K. and Kuijpers, T. W. (2012). Defects in Glanzmann thrombasthenia and LAD-III (LAD-1/v) syndrome: the role of integrin beta1 and beta3 in platelet adhesion to collagen. Blood 119(2): 583-586.
  12. Gutiérrez, L.*, Libregts, S. F.*, de Bruin, A. M., Wensveen, F.M., Papadopoulos, P., van Ijcken, W., Özgür, Z., Philipsen, S. and Nolte, M. A. (2011). Chronic IFNgamma production in mice induces anemia by reducing erythrocyte lifespan and inhibiting erythropoiesis through an IRF1/PU1-axis. Blood 118(9): 2578-2588.
  13. Drogat, B., Kalucka, J., Gutiérrez, L., Hammad, H., Goossens, S., Farhang Ghahremani, M., Bartunkova, S., Haigh, K., Deswarte, K., Nyabi, O., Naessens, M., Ferrara, N., Klingmüller, U., Lambrecht, B. N., Nagy, A., Philipsen, S. and Haigh, J. J. (2010). Vegf regulates embryonic erythroid development through Gata1 modulation. Blood 116(12): 2141-2151.
  14. Borg, J., Papadopoulos, P., Georgitsi, M., Gutierrez, L., Grech, G., Fanis, P., Phylactides, M., Verkerk, A. J., van der Spek, P. J., Scerri, C. A., Cassar, W., Galdies, R., van Ijcken, W., Özgür, Z., Gillemans, N., Hou, J., Bugeja, M., Grosveld, F. G., von Lindern, M., Felice, A. E., Patrinos, G. P. and Philipsen, S. (2010). Haploinsufficiency for the erythroid transcription factor KLF1 causes hereditary persistence of fetal hemoglobin. Nat Genet 42(9): 801-805.
  15. Kozma, G. T., Martelli, F., Verrucci, M., Gutiérrez, L., Migliaccio, G., Sánchez, M., Alfani, E., Philipsen, S. and Migliaccio, A. R. (2010). Dynamic regulation of Gata1 expression during the maturation of conventional dendritic cells. Exp Hematol 38(6): 489-503 e481.
  16. McKimmie, C. S., Fraser, A. R., Hansell, C., Gutiérrez, L., Philipsen, S., Connell, L., Rot, A., Kurowska-Stolarska, M., Carreno, P., Pruenster, M., Chu, C. C., Lombardi, G., Halsey, C., McInnes, I. B., Liew, F. Y., Nibbs, R. J. and Graham, G. J. (2008). Haemopoietic cell expression of the chemokine decoy receptor D6 is dynamic and regulated by GATA1. J Immunol 181: 3353-3363.
  17. Gutiérrez, L.*, Tsukamoto, S.*, Suzuki, M.*, Yamamoto-Mukai, H., Yamamoto, M., Philipsen, S. and Ohneda, K. (2008). Ablation of Gata1 in adult mice results in aplastic crisis, revealing its essential role in steady-state and stress erythropoiesis. Blood 111(8): 4375-4385.
  18. Gutiérrez, L.*, Nikolic, T.*, van Dijk, T. B., Hammad, H., Vos, N., Willart, M., Grosveld, F., Philipsen, S. and Lambrecht, B. N. (2007). Gata1 regulates dendritic-cell development and survival. Blood 110(6): 1933-1941.
  19. Gutiérrez, L., Lindeboom, F., Ferreira, R., Drissen, R., Grosveld, F., Whyatt, D. and Philipsen, S. (2005). A hanging drop culture method to study terminal erythroid differentiation. Exp Hematol 33(10): 1083-1091.
    *Equal contribution
1 Protocol published
Authors:  Vishal Salunkhe, Petros Papadopoulos and Laura Gutiérrez, date: 11/05/2015, view: 22025, Q&A: 4
Megakaryocytes are the precursor cells of platelets and are bona fide resident cells in the bone marrow but extremely low in numbers (~1% of total nucleated cells). Upon terminal differentiation, megakaryocytes increase their size, become ...
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