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Coauthors
Günter Theißen Department of Genetics, Friedrich Schiller University Jena, Germany
3 protocols

Florian Rümpler Department of Genetics, Friedrich Schiller University Jena, Germany
1 protocol
Coreviewers
Alberto Carbonell Instituto de Biología Molecular y Celular de Plantas
2 protocols

Annis Richardson University of California, Berkeley
5 protocols

Antoine Danon Sorbonne Universités
4 protocols

Arsalan Daudi University of California
157 protocols

Editor
Rainer Melzer
  • Assistant Professor, University College Dublin
Research focus
  • Plant science
  • Plant genetics, plant development, crops, evo-devo, transcription factors, biophysics, species diversity.
  • 1 Author merit

Education

Dr. rer. nat., Friedrich Schiller University Jena, 2009

Lab information

Lab homepage: www.ucdflowerpower.org
http://www.ucdflowerpower.org

Publications

1 Protocol published
Character-State Reconstruction to Infer Ancestral Protein-Protein Interaction Patterns
Authors:  Florian Rümpler, Günter Theißen and Rainer Melzer, date: 08/20/2015, view: 5522, Q&A: 0
Protein-protein interactions are at the core of a plethora of developmental, physiological and biochemical processes. Consequently, insights into the origin and evolutionary dynamics of protein-protein interactions may provide information on the ...
2 Protocols reviewed
Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
Authors:  Prarthana Mohanraju, John van der Oost, Martin Jinek and Daan C. Swarts, date: 05/05/2018, view: 1409, Q&A: 0
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification ...
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Chromosome Dosage Analysis in Plants Using Whole Genome Sequencing
Authors:  Ek Han Tan, Luca Comai and Isabelle M. Henry, date: 07/05/2016, view: 4534, Q&A: 0
Relative chromosome dosage, i.e., increases or decreases in the number of copies of specific chromosome regions in one sample versus another, can be determined using aligned read-counts from Illumina sequencing (Henry et al. ...
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9 Protocols edited
Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly
Authors:  Axel Himmelbach, Ines Walde, Martin Mascher and Nils Stein, date: 08/05/2018, view: 346, Q&A: 0
Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution ...
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High Resolution Melting Temperature Analysis to Identify CRISPR/Cas9 Mutants from Arabidopsis
Authors:  Cynthia Denbow, Sonia Carole Ehivet and Sakiko Okumoto, date: 07/20/2018, view: 404, Q&A: 0
CRISPR/Cas9 made targeted mutagenesis and genome editing possible for many plant species. One of the ways that the endonuclease is used for plant genetics is the creation of loss-of-function mutants, which typically result from erroneous DNA repair ...
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