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Coauthors
Fiona Doohan Earth Institute and School of Biology and Environmental Sciences, University College Dublin, Ireland, Ireland,
4 protocols

Lokanadha Gunupuru Molecular Plant-Microbe Interactions Laboratory, School of Biology and Environmental Science, UCD Earth Institute, University College Dublin, Ireland
2 protocols

Steven Scofield USDA-ARS, Crop Production and Pest Control Research Unit and Purdue University, Department of Agronomy, USA
1 protocol
Coreviewers
Aksiniya Asenova University of California, Davis
11 protocols

Isabelle Colas James Hutton Institute
2 protocols

Samik Bhattacharya Osnabrueck University
54 protocols

Susheel Kumar E.I.DuPont India Private Limited
2 protocols

Reviewer
Shahin S. Ali
  • Research scientist, USDA/ARS Sustainable Perennial Crops Lab
Research focus
  • Plant science
  • Molecular Plant-Microbe Interactions
  • 2 Author merit

Education

PhD, University College Dublin, Ireland, 2012

Publications

2 Protocols published
Visual Assessment of the Severity of Fusarium Seedling Blight (FSB) and Fusarium Head Blight (FHB) Disease in Barley
Authors:  Shahin S. Ali, Lokanadha R. Gunupuru and Fiona M. Doohan, date: 06/20/2015, view: 4422, Q&A: 0
Fusarium pathogens are among the most damaging pathogens of cereals. These pathogens have the ability to attack the roots, seedlings, and flowering heads of barley and wheat plants (Simpson et al., 2004). Resulting in yield loss ...
Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves
Authors:  Lokanadha R. Gunupuru, Shahin S. Ali, Fiona M. Doohan and Steven R. Scofield, date: 06/20/2015, view: 5030, Q&A: 0
Virus induced gene silencing (VIGS) is one of the most potent reverse genetics technologies for gene functional characterisation. This method exploits a dsRNA-mediated antiviral defence mechanism in plants. Using this method allows researchers to ...
7 Protocols reviewed
Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9
The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR ...
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A Reliable Assay to Evaluate the Virulence of Aspergillus nidulans Using the Alternative Animal Model Galleria mellonella (Lepidoptera)
The greater wax moth Galleria mellonella has emerged as an effective heterologous host to study fungal pathogenesis and the efficacy of promising antifungal drugs (Mylonakis et al., 2005; Li et al., 2013). Here, a ...
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