Fiona Doohan Earth Institute and School of Biology and Environmental Sciences, University College Dublin, Ireland, Ireland,
4 protocols

Lokanadha Gunupuru Molecular Plant-Microbe Interactions Laboratory, School of Biology and Environmental Science, UCD Earth Institute, University College Dublin, Ireland
2 protocols

Steven Scofield USDA-ARS, Crop Production and Pest Control Research Unit and Purdue University, Department of Agronomy, USA
1 protocol
Aksiniya Asenova University of California, Davis
15 protocols

Isabelle Colas James Hutton Institute
2 protocols

Nayidu Naghabushana University of Saskatchewan
1 protocol

Samik Bhattacharya Osnabrueck University
61 protocols

Reviewer
Shahin S. Ali
  • Research scientist, USDA/ARS Sustainable Perennial Crops Lab
Research focus
  • Plant science
  • Molecular Plant-Microbe Interactions
  • 2 Author merit

Education

PhD, University College Dublin, Ireland, 2012

Publications

2 Protocols published
Visual Assessment of the Severity of Fusarium Seedling Blight (FSB) and Fusarium Head Blight (FHB) Disease in Barley
Authors:  Shahin S. Ali, Lokanadha R. Gunupuru and Fiona M. Doohan, date: 06/20/2015, view: 7974, Q&A: 0
Fusarium pathogens are among the most damaging pathogens of cereals. These pathogens have the ability to attack the roots, seedlings, and flowering heads of barley and wheat plants (Simpson et al., 2004). Resulting in yield loss ...
Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves
Authors:  Lokanadha R. Gunupuru, Shahin S. Ali, Fiona M. Doohan and Steven R. Scofield, date: 06/20/2015, view: 10078, Q&A: 1
Virus induced gene silencing (VIGS) is one of the most potent reverse genetics technologies for gene functional characterisation. This method exploits a dsRNA-mediated antiviral defence mechanism in plants. Using this method allows researchers to ...
8 Protocols reviewed
Design and Construction of Repacked Soil Columns for Measuring Solute Transport, Plant Growth and Soil Biota
Authors:  Israel Ikoyi and Achim Schmalenberger, date: 01/20/2021, view: 962, Q&A: 0

Researchers face a number of challenges in the construction of soil columns which can affect the outcome of their experiments. The use of intact soil cores closely mimics actual field conditions. However, the excavation of large intact soil cores is

...
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Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9
The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR ...
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