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Xiuren Zhang Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, College Station, USA
1 protocol

Hongliang Zhu China Agricultural University
1 protocol

Liwei Wen College of Food Science and Nutritional Engineering, China Agricultural University, China
1 protocol

Rui Li College of Food Science and Nutritional Engineering, China Agricultural University, China
1 protocol

Claudia Castillo-González
  • Department of Biochemistry and Biophysics, Institute of Plant Genomics and Biotechnology, Texas A&M University, College Station, USA
  • 1 Author merit

Education

Ph.D. candidate in Biochemistry, Texas A&M University, USA

Current position

Ph.D. candidate in Biochemistry, Texas A&M University, USA

Publications

  1. Zhu, H., Zhou, Y., Castillo-González, C., Lu, A., Ge, C., Zhao, Y. T., Duan, L., Li, Z., Axtell, M. J. and Wang, X. J. (2013). Bidirectional processing of pri-miRNAs with branched terminal loops by Arabidopsis Dicer-like1. Nat Struct Mole Biol 20(9): 1106-1115.
1 Protocol published
In vitro Reconstitution Assay of miRNA Biogenesis by Arabidopsis DCL1
Authors:  Tian Wang, Claudia Castillo-González, Lan You, Rui Li, Liwei Wen, Hongliang Zhu and Xiuren Zhang, date: 04/20/2015, view: 4356, Q&A: 0
microRNAs (miRNAs) are small non-coding RNAs, regulating most if not all, biological processes in eukaryotic organisms. miRNAs are initially processed from primary transcripts (pri-miRNAs) to produce miRNA precursors (pre-miRNAs), that are further ...
2 Protocols feedback
Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
Author:  Xiyan Li, date: 07/05/2011, view: 20724, Q&A: 3
Promoter-driven GUS (beta-glucuronidase) activity is the most commonly used technique for tissue-specific expression patterns in Arabidopsis. In this procedure, GUS enzyme converts 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc) to a blue product. The staining is very sensitive. Processed samples can be examined under dissecting microscope or ...
Pollen Fertility/viability Assay Using FDA Staining
Author:  Xiyan Li, date: 05/20/2011, view: 21597, Q&A: 2
Pollen grains can be fertile or sterile by nature. This method stains pollen grains for an enzyme as the vital indicator of membrane integrity. Only fertile grains fluoresce under microscopic examination.