Erika Toda Biological Sciences, Tokyo Metropolitan University, Japan
2 protocols

Yukinosuke Ohnishi Tokyo Metropolitan University
2 protocols

Takashi Okamoto
  • Professor, Department of Biological Sciences, Tokyo Metropolitan University
Research focus
  • Plant science
  • 3 Author merit


Ph.D. in Science, Department of Biological Sciences, Tokyo Metropolitan University, 1998


Since 2015
• Ohnishi, Y., Hoshino, R. and Okamoto, T. (2014). Dynamics of male and female chromatin during karyogamy in rice zygotes. Plant Physiol 165(4): 1533-1543.
• Abiko, M., Furuta, K., Yamauchi, Y., Fujita, C., Taoka, M., Isobe, T. and Okamoto, T. (2013). Identification of proteins enriched in rice egg or sperm cells by single-cell proteomics. PLoS One 8(7): e69578.
• Abiko, M., Maeda, H., Tamura, K., Hara-Nishimura, I. and Okamoto, T. (2013). Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up- or down-regulated genes in zygotes after fertilization. J Exp Bot 64(7): 1927-1940.
• Okamoto, T. (2011). In vitro fertilization with isolated rice gametes: production of zygotes and zygote and embryo culture. Methods Mol Biol 710: 17-27.
• Ohnishi, T., Takanashi, H., Mogi, M., Takahashi, H., Kikuchi, S., Yano, K., Okamoto, T., Fujita, M., Kurata, N. and Tsutsumi, N. (2011). Distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays. Plant Physiol 155(2): 881-891.
• Takeuchi, K., Gyohda, A., Tominaga, M., Kawakatsu, M., Hatakeyama, A., Ishii, N., Shimaya, K., Nishimura, T., Riemann, M., Nick, P., Hashimoto, M., Komano, T., Endo, A., Okamoto, T., Jikumaru, Y., Kamiya, Y., Terakawa, T. and Koshiba, T. (2011). RSOsPR10 expression in response to environmental stresses is regulated antagonistically by jasmonate/ethylene and salicylic acid signaling pathways in rice roots. Plant Cell Physiol 52: 1686–1696.
• Okamoto, T. (2010). Gamete fusion site on the egg cell and autonomous establishment of cell polarity in the zygote. Plant Signal Behav 5(11): 1464-1467.
• Nakajima, K., Uchiumi, T. and Okamoto, T. (2010). Positional relationship between the gamete fusion site and the first division plane in the rice zygote. J. Exp. Bot. 61: 3101-3105.
• Sato, A., Toyooka, K. and Okamoto, T. (2010). Asymmetric cell division of rice zygotes located in embryo sac and produced by in vitro fertilization. Sex Plant Reprod 23(3): 211-217.
• Uchiumi, T. and Okamoto, T. (2010). Rice fruit development is associated with an increased IAA content in pollinated ovaries. Planta 232: 579-592.
• Takanashi, H., Ohnishi, T., Mogi, M., Okamoto, T., Arimura, S. and Tsutsumi, N. (2010). Studies of mitochondrial morphology and DNA amount in the rice egg cell. Curr Genet 56(1): 33-41.
• Wang, S. and Okamoto, T. (2009). Involvement of polypyrimidine tract-binding protein (PTB)-related proteins in pollen germination in Arabidopsis. Plant Cell Physiol 50(2): 179-190.
• Kranz, E., Hoshino, Y. and Okamoto, T. (2008). In vitro fertilization with isolated higher plant gametes. Methods Mol Biol 427: 51-69.
• Uchiumi, T., Uemura, I. and Okamoto, T. (2007). Establishment of an in vitro fertilization system in rice (Oryza sativa L.). Planta 226(3): 581-589.
• Uchiumi, T., Shinkawa, T., Isobe, T. and Okamoto, T. (2007). Identification of the major protein components of rice egg cells. J Plant Res 120(4): 575-579.
• Uchiumi, T., Komatsu, S., Koshiba, T. and Okamoto, T. (2006). Isolation of gametes and central cells from Oryza sativa L. Sex Plant Reprod. 19: 37-45.
• Okamoto, T., Scholten, S., Lörz, H. and Kranz, E. (2005). Identification of genes that are up- or down-regulated in the apical or basal cell of maize two-celled embryos and monitoring their expressions during zygote development by a cell manipulation- and PCR-based approach.Plant Cell Physiol 46: 332-338.
3 Protocols published
Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
Authors:  Erika Toda and Takashi Okamoto, date: 07/20/2020, view: 1015, Q&A: 0
Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered ...
Electro-fusion of Gametes and Subsequent Culture of Zygotes in Rice
Authors:  Erika Toda, Yukinosuke Ohnishi and Takashi Okamoto, date: 12/20/2016, view: 7859, Q&A: 0
Electro-fusion system with isolated gametes has been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, since the female gametophytes of plants are deeply embedded ...
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