Hello,I have two questions. Do I need to mixing the lysate adequately before transfer it to -80? How could I determine the concentration of CHX when I use the cells of rat embryonic fibroblast. Should I treated the cells with CHX with different concentrations for 8 hours and observe the apoptosis markers and some protein with well-known half-life? Thank you very much.
9/2/2015 1:08:52 AM Reply