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Coauthors
Cheng-Hsun Ho
Agricultural Biotechnology Research Center of Academia Sinica
1 protocol
Roberto De Michele CNR-IGV
Institute of Biosciences and Bioresources
1 protocol
Guido Grossmann
Universität Heidelberg
1 protocol
Cindy Cooke
Carnegie Institution for Science
1 protocol
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Wolf B. Frommer
Department of Plant Biology, Carnegie Science, USA
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2
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2 Protocols published
Design and Functional Analysis of Fluorescent Nitrate and Peptide Transporter Activity Sensors in Yeast Cultures
Authors:
Cheng-Hsun Ho
and
Wolf B. Frommer
,
date:
02/05/2016,
view:
3431,
Q&A:
0
This protocol describes the methods used to engineer and deploy genetically encoded fluorescence activity reporters for nitrate and peptide transporter activity in yeast cells. Fusion of the dual-affinity nitrate transceptor CHL1/AtNRT1.1/AtNPF6.3 ...
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Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors
Authors:
Cindy Ast
,
Wolf B. Frommer
,
Guido Grossmann
and
Roberto De Michele
,
date:
01/05/2015,
view:
4508,
Q&A:
0
AmTracs are the first example of “activity sensors”, since they report the activity of ammonium transporters by means of fluorescence readout
in vivo
(De Michele
et al.
, 2013). AmTracs are based on a single fluorescent protein, a ...
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