Holden Maecker Immunity, Transplantation & Infection, School of Medicine, Stanford University, USA
9 protocols

Michael D. Leipold
  • Human Immune Monitoring Center, Stanford University, USA
Research focus
  • Immunology
  • 1 Author merit
Lab Protocol Hub


Ph.D. in Chemistry, University of Utah Salt Lake City, UT, 2003

Current position

Research and Development Engineer, Stanford University, Stanford, USA (11/2010-present)


  1. O'Gorman, W. E., Huang, H., Wei, Y. L., Davis, K. L., Leipold, M. D., Bendall, S. C., Kidd, B. A., Dekker, C. L., Maecker, H. T., Chien, Y. H. and Davis, M. M. (2014). The Split Virus Influenza Vaccine rapidly activates immune cells through Fcgamma receptors. Vaccine 32(45): 5989-5997.
  2. Horowitz, A., Strauss-Albee, D. M., Leipold, M., Kubo, J., Nemat-Gorgani, N., Dogan, O. C., Dekker, C. L., Mackey, S., Maecker, H. and Swan, G. E. (2013). Genetic and environmental determinants of human NK cell diversity revealed by mass cytometry. Sci Transl Med 5(208): 208ra145-208ra145.
  3. Leipold, M. D. and Maecker, H. T. (2012). Mass cytometry: protocol for daily tuning and running cell samples on a CyTOF mass cytometer. J Vis Exp(69): e4398.
  4. Leipold, M. D., Ornatsky, O., Baranov, V., Whitfield, C. and Nitz, M. (2011). Development of mass cytometry methods for bacterial discrimination. Anal Biochem 419(1): 1-8.
  5. Leipold, M. D., Herrera, I., Ornatsky, O., Baranov, V. and Nitz, M. (2009). ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers. J Proteome Res 8(2): 443-449.
  6. Leipold, M. D., Vinogradov, E. and Whitfield, C. (2007). Glycosyltransferases involved in biosynthesis of the outer core region of Escherichia coli lipopolysaccharides exhibit broader substrate specificities than is predicted from lipopolysaccharide structures. J Biol Chem 282(37): 26786-26792.
  7. Leipold, M. D., Kaniuk, N. A. and Whitfield, C. (2007). The C-terminal Domain of the Escherichia coli WaaJ glycosyltransferase is important for catalytic activity and membrane association. J Biol Chem 282(2): 1257-1264.
  8. Leipold, M. D., Workman, H., Muller, J. G., Burrows, C. J. and David, S. S. (2003). Recognition and removal of oxidized guanines in duplex DNA by the base excision repair enzymes hOGG1, yOGG1, and yOGG2. Biochemistry 42(38): 11373-11381.
  9. Burrows, C. J., Muller, J. G., Kornyushyna, O., Luo, W., Duarte, V., Leipold, M. D. and David, S. S. (2002). Structure and potential mutagenicity of new hydantoin products from guanosine and 8-oxo-7,8-dihydroguanine oxidation by transition metals. Environ Health Perspect 110 Suppl 5: 713-717.
  10. Leipold, M. D., Muller, J. G., Burrows, C. J. and David, S. S. (2000). Removal of hydantoin products of 8-oxoguanine oxidation by the Escherichia coli DNA repair enzyme, FPG. Biochemistry 39(48): 14984-14992.
1 Protocol published
Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry
Authors:  Michael D. Leipold and Holden Maecker, date: 01/20/2015, view: 13434, Q&A: 3
Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques ...
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