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Holden Maecker Microbiology & Immunology, Stanford University, Stanford
7 protocols

Dongxia Lin Department of Microbiology and Immunology, Stanford University, USA
1 protocol

Sheena Gupta
  • Human Immune Monitoring Center, Stanford University, USA
  • 4 Author merit
Lab Protocol Hub
2 Protocols published
Intracellular Cytokine Staining (ICS) on Human Lymphocytes or Peripheral Blood Mononuclear Cells (PBMCs)
Authors:  Sheena Gupta and Holden Maecker, date: 04/05/2015, view: 10407, Q&A: 1
Production of cytokines plays an important role in the immune response. Cytokines are involved in many different pathways including the induction of many anti-viral proteins by IFN gamma, the induction of T cell proliferation by IL-2 and the ...
Intracellular Cytokine Staining on PBMCs Using CyTOFTM Mass Cytometry
Authors:  Dongxia Lin, Sheena Gupta and Holden Maecker, date: 01/05/2015, view: 11050, Q&A: 2
In this protocol, we use a CyTOFTM mass cytometry to collect single-cell data on a large number of cytokines/chemokines as well as cell-surface proteins that characterize T cells and other immune cells. The current selected mass window in ...