Melike Çağlayan University of Florida
3 protocols

Julie Horton Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
1 protocol

Samuel H. Wilson
  • Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
  • 3 Author merit
3 Protocols published
In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
Authors:  Melike Çağlayan and Samuel H. Wilson, date: 06/20/2017, view: 3062, Q&A: 0
We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide ...
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells
Authors:  Melike Çağlayan, Julie K. Horton and Samuel H. Wilson, date: 06/05/2015, view: 3780, Q&A: 0
We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, ...
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