B. Poovaiah Graduate Program in Molecular Plant Sciences, Washington State University, USA
1 protocol

Liqun Du Department of Horticulture, Washington State University, USA
1 protocol

Reviewer
Lei Zhang
  • Washington State University
Research focus
  • Plant science
  • 1 Author merit
1 Protocol published
CytoTrap Two-Hybrid Screening Assay
Authors:  Lei Zhang, Liqun Du and B. W. Poovaiah, date: 12/05/2014, view: 7915, Q&A: 0
CytoTrap two-hybrid system provides an alternate strategy to detect protein-protein interactions in yeast. In this system, bait protein is fused with human son of sevenless (hSos) protein (Li et al., 1993), and a cDNA library or prey ...
1 Protocol feedback
In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.
The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...
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