Dušan Veličković Pacific Northwest National Laboratory
1 protocol

Hélène Rogniaux
  • INRA, Biopolymers Interactions Assemblies, France
Research focus
  • Plant science
  • 1 Author merit


Ph.D. in Physical Chemistry at Louis Pasteur University, Strasbourg, France, 2000

Current position

Team leader at the National Institute for Agronomic Research, Nantes, France; Head of the Mass Spectrometry Laboratory, devoted to the structural analysis of plant proteins and polysaccharides (2002-present)

Publications (since 2010)

  1. Ropartz, D., Lemoine, J., Giuliani, A., Bittebiere, Y., Enjalbert, Q., Antoine, R., Dugourd, P., Ralet, M. C. and Rogniaux, H. (2014). Deciphering the structure of isomeric oligosaccharides in a complex mixture by tandem mass spectrometry: photon activation with vacuum ultra-violet brings unique information and enables definitive structure assignment. Anal Chim Acta 807: 84-95.
  2. Velickovic, D., Herdier, H., Philippe, G., Marion, D., Rogniaux, H. and Bakan, B. (2014). Matrix-assisted laser desorption/ionization mass spectrometry imaging: a powerful tool for probing the molecular topology of plant cutin polymer. Plant J 80(5): 926-935.
  3. Velickovic, D., Ropartz, D., Guillon, F., Saulnier, L. and Rogniaux, H. (2014). New insights into the structural and spatial variability of cell-wall polysaccharides during wheat grain development, as revealed through MALDI mass spectrometry imaging. J Exp Bot 65(8): 2079-2091.
  4. Garnier, M., Carrier, G., Rogniaux, H., Nicolau, E., Bougaran, G., Saint-Jean, B. and Cadoret, J. P. (2014). Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea. J Proteomics 105: 107-120.
  5. Lupi, R., Masci, S., Rogniaux, H., Tranquet, O., Brossard, C., Lafiandra, D., Moneret-Vautrin, D., Denery-Papini, S. and Larré, C. (2014). Assessment of the allergenicity of soluble fractions from GM and commercial genotypes of wheats. J Cereal Sci 60 (1): 179-186.
  6. Suliman, M., Chateigner-Boutin, A. L., Francin-Allami, M., Partier, A., Bouchet, B., Salse, J., Pont, C., Marion, J., Rogniaux, H., Tessier, D., Guillon, F. and Larre, C. (2013). Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm. J Proteomics 78: 508-521.
  7. Lupi, R., Denery-Papini, S., Rogniaux, H., Lafiandra, D., Rizzi, C., De Carli, M., Moneret-Vautrin, D. A., Masci, S. and Larre, C. (2013). How much does transgenesis affect wheat allergenicity?: Assessment in two GM lines over-expressing endogenous genes. J Proteomics 80: 281-291.
  8. Konozy, E. H., Rogniaux, H., Causse, M. and Faurobert, M. (2013). Proteomic analysis of tomato (Solanum lycopersicum) secretome. J Plant Res 126(2): 251-266.
  9. Repetto, O., Rogniaux, H., Larre, C., Thompson, R. and Gallardo, K. (2012). The seed nuclear proteome. Front Plant Sci 3: 289.
  10. Ropartz, D., Bodet, P. E., Przybylski, C., Gonnet, F., Daniel, R., Fer, M., Helbert, W., Bertrand, D. and Rogniaux, H. (2011). Performance evaluation on a wide set of matrix-assisted laser desorption ionization matrices for the detection of oligosaccharides in a high-throughput mass spectrometric screening of carbohydrate depolymerizing enzymes. Rapid Commun Mass Spectrom 25(14): 2059-2070.
  11. Larre, C., Lupi, R., Gombaud, G., Brossard, C., Branlard, G., Moneret-Vautrin, D. A., Rogniaux, H. and Denery-Papini, S. (2011). Assessment of allergenicity of diploid and hexaploid wheat genotypes: identification of allergens in the albumin/globulin fraction. J Proteomics 74(8): 1279-1289.
  12. Bancel, E., Rogniaux, H., Debiton, C., Chambon, C. and Branlard, G. (2010). Extraction and proteome analysis of starch granule-associated proteins in mature wheat kernel (Triticum aestivum L.). J Proteome Res 9(6): 3299-3310.
  13. Larre, C., Penninck, S., Bouchet, B., Lollier, V., Tranquet, O., Denery-Papini, S., Guillon, F. and Rogniaux, H. (2010). Brachypodium distachyon grain: identification and subcellular localization of storage proteins. J Exp Bot 61(6): 1771-1783.
  14. Tessier, D., Yclon, P., Jacquemin, I., Larre, C. and Rogniaux, H. (2010). OVNIp: an open source application facilitating the interpretation, the validation and the edition of proteomics data generated by MS analyses and de novo sequencing. Proteomics 10(9): 1794-1801.
1 Protocol published
In situ Digestion of Wheat Cell-wall Polysaccharides
Authors:  Dušan Veličković and Hélène Rogniaux, date: 12/05/2014, view: 9481, Q&A: 2
Cell walls of the wheat endosperm are mostly composed of arabinoxylans (AX) and mixed (1→3), (1→4)-β-glucans (BG) (Saulnier et al., 2012). Here, we present an optimized protocol to degrade enzymatically these cell-wall polysaccharides into ...
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