Naotaka Nishimura Department of Molecular Genetics, Kumamoto University, Japan
1 protocol
Juan Facundo Ayala Instituto de Nanociencia y Nanotecnología
25 protocols

Toshitsugu Fujita Hirosaki University Graduate School of Medicine
6 protocols

Reviewer
Masato Yano
  • Kumamoto Health Science University
Research focus
  • Cell biology
  • 1 Author merit
1 Protocol published
Separation of the Inner and Outer Mitochondrial Membrane in HeLa Cells
Authors:  Naotaka Nishimura and Masato Yano, date: 11/20/2014, view: 23168, Q&A: 1
Mitochondria are organelles that have important functions in oxidative phosphorylation, fatty acid oxidation and apoptosis signaling. They have two distinct membranes, outer membrane (OM) and inner membrane (IM). IM contains respiratory chain ...
2 Protocols reviewed
Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
Authors:  Ryan R. Cupo and James Shorter, date: 12/05/2020, view: 1614, Q&A: 0

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the

...
More >
Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae
Authors:  Louise Thines and Pierre Morsomme, date: 03/05/2020, view: 1822, Q&A: 0
Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2). ...
More >
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.