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Coauthors
Frances Separovic The University of Melbourne
2 protocols

Neil O’Brien-Simpson Melbourne Dental School, The University of Melbourne, Australia
2 protocols

Eric Reynolds Melbourne Dental School, The University of Melbourne, Australia
1 protocol

Katrina Walsh Melbourne Dental School, The University of Melbourne, Australia
1 protocol
Coreviewers
Alka Mehra Institute of Genomics and Integrative Biology
18 protocols

Anca Savulescu University of Cape Town
11 protocols

Arsalan Daudi University of California
156 protocols

Ashish Sethi University of Melbourne
1 protocol

Editor
Marc-Antoine Sani
  • Research Fellow, University of Melbourne
Research focus
  • Biophysics
  • Structural biology, NMR spectroscopy, lipid-protein interactions, biophysics
  • 2 Author merit

Education

PhD, University of Bordeaux / Umea University, 2008

Lab information

Group leader: Frances Separovic

Publications

https://scholar.google.com.au/citations?hl=en&user=Er-RAsQAAAAJ&view_op=list_works&sortby=pubdate

2 Protocols published
Bacterial Fluorescent-dextran Diffusion Assay
Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance ...
Dye Release Experiments with Dextran Loaded Vesicles
Authors:  Marc-Antoine Sani, Neil M. O’Brien-Simpson and Frances Separovic, date: 07/20/2014, view: 4217, Q&A: 0
Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously ...
4 Protocols reviewed
Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels
Authors:  Seung-Kwon Ha, Govind Nair, Martina Absinta, Nicholas J. Luciano and Daniel S. Reich, date: 04/20/2018, view: 513, Q&A: 0
In this protocol, we describe a method to visualize and map dural lymphatic vessels in-vivo using magnetic resonance imaging (MRI) and ex-vivo using histopathological techniques. While MRI protocols for routine imaging of meningeal ...
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Bioelectrospray Methodology for Dissection of the Host-pathogen Interaction in Human Tuberculosis
Authors:  Liku B Tezera, Magdalena K Bielecka and Paul T Elkington, date: 07/20/2017, view: 2192, Q&A: 0
Standard cell culture models have been used to investigate disease pathology and to test new therapies for over fifty years. However, these model systems have often failed to mimic the changes occurring within three-dimensional (3-D) space where ...
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6 Protocols edited
Xenopus laevis Oocytes Preparation for in-Cell EPR Spectroscopy
Authors:  Laura John and Malte Drescher, date: 04/05/2018, view: 449, Q&A: 0
One of the most exciting perspectives for studying bio-macromolecules comes from the emerging field of in-cell spectroscopy, which enables to determine the structure and dynamics of bio-macromolecules in the cell. In-cell electron paramagnetic ...
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Preparation of Everted Membrane Vesicles from Escherichia coli Cells
Author:  Marina Verkhovskaya, date: 05/05/2017, view: 1913, Q&A: 0
The protocol for obtaining electrically sealed membrane vesicles from E. coli cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by ...
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