Frances Separovic The University of Melbourne
2 protocols

Neil O’Brien-Simpson Melbourne Dental School, The University of Melbourne, Australia
2 protocols

Eric Reynolds Melbourne Dental School, The University of Melbourne, Australia
1 protocol

Katrina Walsh Melbourne Dental School, The University of Melbourne, Australia
1 protocol
Agnieszka Zienkiewicz Nicolaus Copernicus University
22 protocols

Alka Mehra Institute of Genomics and Integrative Biology
39 protocols

Anca Savulescu University of Cape Town
22 protocols

Arsalan Daudi University of California
157 protocols

Editor
Marc-Antoine Sani
  • Research Fellow, University of Melbourne
Research focus
  • Biophysics
  • Structural biology, NMR spectroscopy, lipid-protein interactions, biophysics
  • 2 Author merit

Education

PhD, University of Bordeaux / Umea University, 2008

Lab information

Group leader: Frances Separovic

Publications

2 Protocols published
Bacterial Fluorescent-dextran Diffusion Assay
Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance ...
Dye Release Experiments with Dextran Loaded Vesicles
Authors:  Marc-Antoine Sani, Neil M. O’Brien-Simpson and Frances Separovic, date: 07/20/2014, view: 8129, Q&A: 0
Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously ...
6 Protocols reviewed
Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
Authors:  Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu, date: 02/20/2021, view: 1155, Q&A: 0

Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules

...
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Measurement of Protein-Protein Interactions through Microscale Thermophoresis (MST)
Authors:  Magnez Romain, Bryan Thiroux, Morgane Tardy, Bruno Quesnel and Xavier Thuru, date: 04/05/2020, view: 2567, Q&A: 0
The binding interactions of PD-1 and PD-L1 have been studied by surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) over the past few years, but these investigations resulted in controversy regarding the values of the ...
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7 Protocols edited
In vivo and in vitro 31P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in Hebeloma cylindrosporum in Response to Plant Roots Signals
We used in vivo and in vitro phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus Hebeloma ...
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Xenopus laevis Oocytes Preparation for in-Cell EPR Spectroscopy
Authors:  Laura John and Malte Drescher, date: 04/05/2018, view: 5295, Q&A: 0
One of the most exciting perspectives for studying bio-macromolecules comes from the emerging field of in-cell spectroscopy, which enables to determine the structure and dynamics of bio-macromolecules in the cell. In-cell electron paramagnetic ...
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