Marja Jäättelä Cell Death and Metabolism Department, Danish Cancer Society Research Center, Denmark
1 protocol

Nikolaj Petersen Orphazyme ApS, Orphazyme ApS, Denmark
1 protocol

Thomas Kirkegaard
  • Orphazyme ApS, Orphazyme ApS, Denmark
  • 1 Author merit
1 Protocol published
Lysosomal Stability Assay
Authors:  Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä, date: 06/20/2014, view: 9157, Q&A: 0
This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the ...