Thomas Kirkegaard —BIO-PROTOCOL

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Marja Jäättelä Cell Death and Metabolism Department, Danish Cancer Society Research Center, Denmark
1 protocol

Nikolaj Petersen Orphazyme ApS, Orphazyme ApS, Denmark
1 protocol

Thomas Kirkegaard

Orphazyme ApS, Orphazyme ApS, Denmark

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1 Protocol published

Lysosomal Stability Assay

Authors: Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä, date: 06/20/2014, view: 9662, Q&A: 0

This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the ...

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