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Marja Jäättelä
Cell Death and Metabolism Department, Danish Cancer Society Research Center, Denmark
1 protocol
Nikolaj Petersen
Orphazyme ApS, Orphazyme ApS, Denmark
1 protocol
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Thomas Kirkegaard
Orphazyme ApS, Orphazyme ApS, Denmark
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1 Protocol published
Lysosomal Stability Assay
Authors:
Nikolaj H. T. Petersen
,
Thomas Kirkegaard
and
Marja Jäättelä
,
date:
06/20/2014,
view:
10168,
Q&A:
0
This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.
AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the ...
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