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James Murphy
Cell Signalling and Cell Death Division, The Walter and Eliza Hall Institute of Medical Research and Department of Medical Biology, University of Melbourne, Australia
1 protocol
Jeffrey Babon
Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research and Department of Medical Biology, University of Melbourne, Australia
1 protocol
John Silke
Cell Signalling and Cell Death Division, The Walter and Eliza Hall Institute of Medical Research and Department of Medical Biology, University of Melbourne, Australia
1 protocol
Nicos Nicola
Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research and Department of Medical Biology, University of Melbourne, Australia
1 protocol
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Isabelle S Lucet
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, and The Walter and Eliza Hall Institute of Medical Research and Department of Medical Biology, University of Melbourne, Australia
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1 Protocol published
Determination of Pseudokinase-ligand Interaction by a Fluorescence-based Thermal Shift Assay
Authors:
Isabelle S Lucet
,
Joanne M. Hildebrand
,
Peter E. Czabotar
,
Jian-Guo Zhang
,
Nicos A. Nicola
,
John Silke
,
Jeffrey J. Babon
and
James M. Murphy
,
date:
06/05/2014,
view:
8903,
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0
This protocol describes a robust technique for the measurement of pseudokinase-ligand interaction by a fluorescence-based Thermal Shift Assay (TSA). Pseudokinases are kinase-like proteins that have recently emerged as crucial regulators of signal ...
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