David Paul —BIO-PROTOCOL

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Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol

Ali Kermani University of Michigan, Ann Arbor
8 protocols

Amriti Lulla UT MD Anderson Cancer Center
6 protocols

Arvind Chandel School of Medicine The University of Tokyo
2 protocols

Chang Ho Lee Dankook University
4 protocols

Editor

David Paul

Post-Doc, MRC Laboratory of Molecular Biology

Research focus

Molecular biology
Molecular Biology
Virology
Membranes

1 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Heidelberg, 2013

Lab information

McMahon Lab
http://www.endocytosis.org

Publications

https://scholar.google.co.uk/citations?user=TXxRtLsAAAAJ&hl=en

1 Protocol published

Purification of HCV-remodeled and Control ER Membranes

Authors: David Paul and Ralf Bartenschlager, date: 05/20/2014, view: 6649, Q&A: 0

As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...

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8 Protocols reviewed

In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication

Authors: Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 7043, Q&A: 0

The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...

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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes

Authors: George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 11632, Q&A: 1

The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...

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33 Protocols edited

A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells

Authors: Amanda L. Koch, Tatsuya Morisaki and Timothy J. Stasevich, date: 07/20/2021, view: 821, Q&A: 0

Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This

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Simplified Epigenome Profiling Using Antibody-tethered Tagmentation

Authors: Steven Henikoff, Jorja G. Henikoff and Kami Ahmad, date: 06/05/2021, view: 1288, Q&A: 0

We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples

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