David Paul —BIO-PROTOCOL

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Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol

Ali Kermani University of Michigan, Ann Arbor
5 protocols

Amriti Lulla UT MD Anderson Cancer Center
6 protocols

Arvind Chandel School of Medicine The University of Tokyo
0 protocol

Chang Ho Lee Dankook University
4 protocols

Editor

David Paul

Post-Doc, MRC Laboratory of Molecular Biology

Research focus

Molecular biology
Molecular Biology
Virology
Membranes

1 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Heidelberg, 2013

Lab information

McMahon Lab
http://www.endocytosis.org

Publications

https://scholar.google.co.uk/citations?user=TXxRtLsAAAAJ&hl=en

1 Protocol published

Purification of HCV-remodeled and Control ER Membranes

Authors: David Paul and Ralf Bartenschlager, date: 05/20/2014, view: 6149, Q&A: 0

As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...

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8 Protocols reviewed

In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication

Authors: Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 6163, Q&A: 0

The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...

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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes

Authors: George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 9528, Q&A: 1

The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...

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27 Protocols edited

In vitro AMPylation/Adenylylation of Alpha-synuclein by HYPE/FICD

Authors: Ali Camara, Anwesha Sanyal, Sayan Dutta, Jean-Christophe Rochet and Seema Mattoo, date: 09/20/2020, view: 369, Q&A: 0

One of the major histopathological hallmarks of Parkinson’s disease are Lewy bodies (LBs) –cytoplasmic inclusions, enriched with fibrillar forms of the presynaptic protein alpha-synuclein (α-syn). Progressive deposition of α-syn into LBs is enabled ...

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Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein

Authors: Sayan Dutta, Ben G. Watson, Seema Mattoo and Jean-Christophe Rochet, date: 07/20/2020, view: 791, Q&A: 0

Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on ...

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