David Paul —BIO-PROTOCOL

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Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol

Ali Kermani University of Michigan, Ann Arbor
3 protocols

Amriti Lulla UT MD Anderson Cancer Center
6 protocols

Chang Ho Lee Dankook University
4 protocols

Clara Lubeseder-Martellato Technical University of Munich
6 protocols

Editor

David Paul

Post-Doc, MRC Laboratory of Molecular Biology

Research focus

Molecular biology
Molecular Biology
Virology
Membranes

1 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Heidelberg, 2013

Lab information

McMahon Lab
http://www.endocytosis.org

Publications

https://scholar.google.co.uk/citations?user=TXxRtLsAAAAJ&hl=en

1 Protocol published

Purification of HCV-remodeled and Control ER Membranes

Authors: David Paul and Ralf Bartenschlager, date: 05/20/2014, view: 5388, Q&A: 0

As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...

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8 Protocols reviewed

In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication

Authors: Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 5155, Q&A: 0

The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...

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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes

Authors: George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 6048, Q&A: 0

The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...

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14 Protocols edited

F-actin Bundle Sedimentation Assay

Authors: Shan-Shan Lin, Mei-Chun Chuang and Ya-Wen Liu, date: 11/05/2019, view: 286, Q&A: 0

Understanding the molecular mechanism governing the higher-order regulation of actin dynamics requires chemically-defined and quantitative assays. Recently, the membrane remodeling large GTPase, dynamin, has been identified as a new actin ...

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Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale

Authors: Junsik Sung, Chunhua Yang, Emilie Viennois, Mingzhen Zhang and Didier Merlin, date: 10/05/2019, view: 623, Q&A: 1

Factors implicated in the pathophysiology of intestinal inflammation include defects in intestinal epithelial barrier function, abnormal immune responses, and activities of the gut microbiota. Current agents used to treat human Inflammatory Bowels ...

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