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Coauthors
Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol
Coreviewers
Chang Ho Lee Dankook University
4 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
41 protocols

Jose Reyes-Darias Vircell S.L.
4 protocols

Longping Tse University of North Carolina at Chapel Hill
16 protocols

Editor
David Paul
  • Post-Doc, Medical Research Council
Research focus
  • Molecular biology
  • Molecular Biology
    Virology
    Membranes
  • 1 Author merit

Education

PhD, University of Heidelberg, 2013

Lab information

McMahon Lab
www.endocytosis.org

Publications

https://scholar.google.co.uk/citations?user=TXxRtLsAAAAJ&hl=en

1 Protocol published
Purification of HCV-remodeled and Control ER Membranes
Authors:  David Paul and Ralf Bartenschlager, date: 05/20/2014, view: 3688, Q&A: 0
As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...
8 Protocols reviewed
In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Authors:  Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 2383, Q&A: 0
The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...
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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
Authors:  George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 3032, Q&A: 0
The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...
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2 Protocols edited
HCV Reporter System (Viral Infection-Activated Split-Intein-Mediated Reporter System) for Testing Virus Cell-to-cell Transmission ex-vivo
Authors:  Fanfan Zhao, Ting Zhao, Libin Deng, Dawei Lv, Xiaolong Zhang, Xiaoyu Pan, Jun Xu and Gang Long, date: 08/05/2018, view: 157, Q&A: 0
Hepatitis C virus (HCV) spread involves two distinct entry pathways: cell-free transmission and cell-to-cell transmission. Cell-to-cell transmission is not only an efficient way for viruses to spread but also an effective method for escaping ...
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Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast
Authors:  Isabel Fernández de Castro and Cristina Risco, date: 04/20/2018, view: 760, Q&A: 0
Positive-stranded (+) RNA viruses are intracellular pathogens in humans, animals and plants. To build viral replicase complexes (VRCs) viruses manipulate lipid flows and reorganize subcellular membranes. Redesigned membranes concentrate viral and ...
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