David Paul —BIO-PROTOCOL

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Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol

Ali Kermani University of Michigan, Ann Arbor
4 protocols

Amriti Lulla UT MD Anderson Cancer Center
6 protocols

Chang Ho Lee Dankook University
4 protocols

Clara Lubeseder-Martellato Technical University of Munich
6 protocols

Editor

David Paul

Post-Doc, MRC Laboratory of Molecular Biology

Research focus

Molecular biology
Molecular Biology
Virology
Membranes

1 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Heidelberg, 2013

Lab information

McMahon Lab
http://www.endocytosis.org

Publications

https://scholar.google.co.uk/citations?user=TXxRtLsAAAAJ&hl=en

1 Protocol published

Purification of HCV-remodeled and Control ER Membranes

Authors: David Paul and Ralf Bartenschlager, date: 05/20/2014, view: 5491, Q&A: 0

As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...

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8 Protocols reviewed

In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication

Authors: Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 5319, Q&A: 0

The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...

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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes

Authors: George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 6239, Q&A: 0

The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...

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16 Protocols edited

Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels

Authors: Tzemach Aouizerat, Aren M. Maeir, Yitzhak Paz, Yuval Gadot, Amir Szitenberg, Sivan Alkalay-Oren, Shunit Coppenhagen-Glazer, Michael Klutstein and Ronen Hazan, date: 01/05/2020, view: 415, Q&A: 0

Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ...

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Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA

Authors: Martin Wegner, Koraljka Husnjak and Manuel Kaulich, date: 01/05/2020, view: 380, Q&A: 0

Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored ...

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