Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol
Ali Kermani University of Michigan, Ann Arbor
1 protocol

Chang Ho Lee Dankook University
4 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
42 protocols

Jan-Ulrik Dahl Illinois State University
2 protocols

Editor
David Paul
  • Post-Doc, Medical Research Council
Research focus
  • Molecular biology
  • Molecular Biology
    Virology
    Membranes
  • 1 Author merit

Education

PhD, University of Heidelberg, 2013

Lab information

McMahon Lab
http://www.endocytosis.org

Publications

1 Protocol published
Purification of HCV-remodeled and Control ER Membranes
Authors:  David Paul and Ralf Bartenschlager, date: 05/20/2014, view: 4106, Q&A: 0
As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...
8 Protocols reviewed
In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Authors:  Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 3118, Q&A: 0
The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...
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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
Authors:  George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 3890, Q&A: 0
The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...
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5 Protocols edited
In vitro Membrane Interaction and Liposome Fusion Assays Using Recombinant Hepatitis C Virus Envelope Protein E2
In order to study the mechanism underlying the Hepatitis C Virus (HCV) fusion process we have performed assays using phospholipid liposomes and a truncated form of E2 protein, E2661 (amino acids 384-661 of the HCV polyprotein) lacking the ...
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Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2 Ectodomain Produced in a Baculovirus Expression System
In this protocol, we describe the production and purification of the ectodomain of the E2661 envelope protein (amino acids 384-661) of the Hepatitis C virus, which plays a fundamental role in the entry of the virus into the host cell. ...
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