David Paul —BIO-PROTOCOL

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Ralf Bartenschlager Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Germany
1 protocol

Ali Kermani University of Michigan, Ann Arbor
1 protocol

Chang Ho Lee Dankook University
4 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
42 protocols

Jan-Ulrik Dahl Illinois State University
2 protocols

Editor

David Paul

Post-Doc, Medical Research Council

Research focus

Molecular biology
Molecular Biology
Virology
Membranes

1 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Heidelberg, 2013

Lab information

McMahon Lab
http://www.endocytosis.org

Publications

https://scholar.google.co.uk/citations?user=TXxRtLsAAAAJ&hl=en

1 Protocol published

Purification of HCV-remodeled and Control ER Membranes

Authors: David Paul and Ralf Bartenschlager, date: 05/20/2014, view: 4106, Q&A: 0

As for all positive strand RNA viruses, hepatitis C virus (HCV) RNA replication is tightly associated with rearranged host cell membranes, termed viral replication factories. However, up to now little is known about both viral and cellular ...

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8 Protocols reviewed

In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication

Authors: Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 3118, Q&A: 0

The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...

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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes

Authors: George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 3890, Q&A: 0

The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...

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5 Protocols edited

In vitro Membrane Interaction and Liposome Fusion Assays Using Recombinant Hepatitis C Virus Envelope Protein E2

Authors: Belén Yélamos, Mar Rodríguez-Rodríguez, Francisco Gavilanes and Julián Gómez-Gutiérrez, date: 12/05/2018, view: 61, Q&A: 0

In order to study the mechanism underlying the Hepatitis C Virus (HCV) fusion process we have performed assays using phospholipid liposomes and a truncated form of E2 protein, E2₆₆₁ (amino acids 384-661 of the HCV polyprotein) lacking the ...

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Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2 Ectodomain Produced in a Baculovirus Expression System

Authors: Julián Gómez-Gutiérrez, Mar Rodríguez-Rodríguez, Francisco Gavilanes and Belén Yélamos, date: 10/05/2018, view: 308, Q&A: 0

In this protocol, we describe the production and purification of the ectodomain of the E2₆₆₁ envelope protein (amino acids 384-661) of the Hepatitis C virus, which plays a fundamental role in the entry of the virus into the host cell. ...

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