Leslie Knipling National Institutes of Health Bethesda, MD
1 protocol

Alice Boulanger National Institutes of Health Bethesda
1 protocol

Meng-Lun Hsieh National Institutes of Health Bethesda, MD
1 protocol

Scott Stibitz Center For Biologics Evaluation and Research, Food and Drug Administration, USA
1 protocol

Deborah M. Hinton
  • Gene Expression and Regulation Section, Laboratory of Cell and Molecular Biology, NIDDK, National Institutes of Health, USA
Research focus
  • Microbiology
  • 2 Author merit
2 Protocols published
Combining Gel Retardation and Footprinting to Determine Protein-DNA Interactions of Specific and/or Less Stable Complexes
Authors:  Meng-Lun Hsieh, Alice Boulanger, Leslie G. Knipling and Deborah M. Hinton, date: 12/05/2020, view: 554, Q&A: 0

DNA footprinting is a classic technique to investigate protein-DNA interactions. However, traditional footprinting protocols can be unsuccessful or difficult to interpret if the binding of the protein to the DNA is weak, the protein has a fast

...
Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Authors:  Qing Chen, Alice Boulanger, Deborah M. Hinton and Scott Stibitz, date: 11/20/2013, view: 9072, Q&A: 0
Protein phosphorylation plays a central role in signal transduction in bacteria. However, separation and detection of the phosphorylated protein from its nonphosphorylated form remain challenging. Here we describe a method to detect phosphorylation ...
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